Objective To improve both the technique and the clinical application of CT-guided percutaneous aspiration biopsy for sacral lesions. Methods CT-guided percutaneous aspiration biopsy was performed in 21 patients with sacral lesions. By using Ackerman drilling needle, trigger type biopsy needle or Chiba needle, both bony and non-bony specimens were collected. Results The procedure was successfully done in all patients and the pathologic diagnosis was obtained in 95.2%. Failure occurred in one case as only necrotic tissue and blood clot were collected in biopsy. Conclusion As a simple, safe and reliable technique, CT-guided percutaneous aspiration biopsy is very helpful in making correct preoperative diagnosis, providing important preoperative reference for the surgeons.

Objective To discuss the application of nursing care in CT-guided percutaneous biopsy of the pancreas.Methods The perioperative nursing measures were carried out in 21 patients receiving percutaneous biopsy of the pancreas under CT-guidance.Active,effective and comprehensive nursing procedures were adopted to closely cooperate with the whole process of percutaneous biopsy as far as possible.Results All the patients could actively cooperate with the physician during the whole process of percutaneous biopsy and the surgery was successfully completed in all patients.The technical success rate with only single puncture was 100%.No obvious complications occurred after the procedure.Conclusion In order to ensure that the patient will be able to cooperate with the CT-guided percutaneous biopsy of the pancreas,that the operation time can be shortened and that the postoperative complications can be avoided,perioperative nursing care is indispensable.

The methylation of CpG island in promoter region of E-cad gene which usually leads to inactivation of E-cad gene is closely related to the occurrence, development, invasion, metastasis and prognosis of a variety of gastrointestinal cancer, such as esophageal carcinoma, gastric carcinoma, hepatocellular carcinoma,cholangiocellular carcinoma, carcinoma of gallbladder, pancreatic carcinoma and colorectal cancer. Further study would probably guide the early diagnosis, gene therapy, prophylaxis and predicting recurrence of tumor metastasis of gastrointestinal cancer.

Objective To investigate effect of Splenic Aminopeptide Oral Lyophilized Powder on urinary retinol-binding protein and CRP in children of mycoplasma pneumonia infection with asthma .Methods 60 cases were randomly divided into 2 groups, 30 cases of control group were given conventional anti-inflammatory treatment, azithromycin.30 patients of Experimental group were treated with Spleen Aminopeptide Oral Lyophilized Powder treatment, two groups were treated for 7 days.30 cases of healthy children were selected, given no any treatment.After treatment, urinary retinol binding protein, blood urea nitrogen, creatinine, cough, wheezing symptom recovery, C-reactive protein, blood and other changes were compared between two groups.ResuIts Two groups of children with urinary retinol binding protein, urea nitrogen, creatinine levels were improved in different degrees, and in experimental group, urinary retinol binding protein, urea nitrogen and creatinine decreased significantly compared with control group (P<0.05).Two groups of children with asthma and wheeze improved in different degrees, and cough wheeze, cough and wheeze recovery time of experimental group had more improvement than those of control group ( P<0.05 ) .Two group of C reactive protein and white blood cell level both improved in different degrees, and the experimental group of C reactive protein and white blood cell levels were significantly lower than those of control group (P<0.05).ConcIusion Spleen Aminopeptide Oral Lyophilized Powder can significantly improve the infection status of children with mycoplasma pneumonia, and relieve cough and wheeze symptoms, lower urinary retinol binding protein and C reactive protein level, improve kidney function damage induced by hypoxia.

Breast cancer is one of reproduction-endocrine related tumors, so the risk factors including environmental and genetic factors which can disrupt anabolism and function of estradiol may increase the risk of breast cancer. Persistent environmental endocrine disruptor chemical (EDCs) can disrupt anabolism and function of endogenous hormone and the degree of disruption may depend on genetic polymorphism. EDCs and metabolic enzyme genetic polymorphism were discussed in this paper. To study the risk factors of breast cancer, both of the environmental factors and the genetic factors should be included.

It is difficult to treat the internal carotid arterial (ICA) wall disease between C2 to C7 segment ( the definition of ICA by E. Bouthillier,1996) with present interventional radiological measurements , includling pseudoaneurysm, giant or wide neck aneurysm and complicated carotid cavernous fistula. Some literatures have reported a few cases treated with coronary covered stent (e.g. Jomed coronary stent, Symbiot coronary stent), with favorable results . However, these kinds of coronary covered stents are too rigid, with uneasinees to pass through in tortuous ICA, and often resulting in injury of vascular wall. The utility of covered stent in intracranial artery also caused controversies of exclusion of origins of arterial branches. We analyzed the functional anatomy of ICA and reviewed our experimental results and primarily clinical application on professional intracranial covered stent with special designed structure and delivery system for ICA, in order to explore the possibility of utility of covered stent in intracranial arteries.

Phenotypic conversion process of cells in blood vessels occurred after percutaneous transluminal angioplasty (PTA), which is similar to “recall” of angiogenesis. This article is an attempt in reviewing the major themes and more recent findings dealing with the phenotypic conversion and cellular source in three layers of arterial wall after PTA in the adult.

Objective To develop a method to determine the content of ketoconazole in the disinfectant by HPLC. Methods The separation was carried out on an ODS C18 column. Methanol-water (80+20) was used as the mobile phases. The flow rate was 1.00 ml/min, the detection wavelength was 239 nm. Results The linearity was obtained in the range of 1.00-100 ?g/ml for ketoconazole, the average recovery rates were 98.1%-99.8%. The difference in a day and in another day was less than 2%. The detection limit was 1.08 ?g/ml. Conclusion This method is simple, accurate, rapid and can be used as a quantitative analysis method for ketoconazole.

Objective To set up an isolated limp perfusion (LIP) model of rabbit approximately asin the physiological state. Methods Measuring and calculating the average speed of blood circulation in rabbit's femoral artery with ultrasound, building up an isolated limp of perfusion system with a mini membrane oxygenator and a mini pump. Observing the values of blood analyses (pH, PO 2 and PCO 2) at prior , mid and post operation with differential exchanging speeds of oxygen, and probabilities of circulation failure with differential blood flow speeds, and the glucose change at several points of time and the difference between the groups with and without compensating glucose, and the dimensions of hind leg prior and post operation. Results Results of ultrasound showed that there was no difference in parameter between bilateral femoral arteries giving no effect on the results of differential groups. Total average flow speed was (12.0?1.8) ml/min. Blood analysis: the perfusion without oxygen exchange showed pH was lower than preperfusion and physiological low threshold. PO 2 was below the normal standard, PCO 2 was higher than prepefursion and physiological upper threshold. 100ml/min of oxygen exchange showed pH, PO 2 and PCO 2 were rearly normal post perfusion and 200ml/min group showed pH and PO 2 obviously higher than the normal physiological value. The gulcose was lower than the normal at 30min after perfusion, but could be recoveried under the compensation of glucose. There was significant difference between the groups processing with and without glucose. There was no circulation failure when the flow speed was in 10ml≤V

The coronary covered stents have been more and more used to treat intracranial arterial diseases for recent years,and the hot point in study has gradually changed from clinical application of coronary covered stents to the fundamental and clinical trial of specially-designed intracranial covered stentsThe covered stents are mainly employed for the treatment of intracranial arterial disorders,including giant,wide-necked or minute cerebral aneurysms,intracranial pseudoaneurysms caused by a variety of reasons,fusiform or dissecting aneurysms of vertebral-basic artery,and internal carotid cavernous fistula,etc.It is very difficult for current surgical or endovascular treatment,both materially and technically,to deal with the above mentioned intracranial disorders.The covered stent can restore the anatomic shape of artery by direct sealing the aneurysmal neck and the fistula.The complications of covered stent treatment include defect of cranial nerves,occlusion of parent artery or branches.This article aims to review the related medical literature published both at home and abroad,to make a retrospective analysis of the therapeutic results,and to put forward some issues of common interest.