Objective To evaluate the clinical effect of a combination application of meglumine adenosine cyclophosphate and metoprolol in chronic heart failure.Methods Put 50 patients divided into two group randomly:group A(treated group) which has 26 cases and group B(control group) which has 24 cases.Patients of group B will receive a basal treatment in a routine way while patients of group A will receive a basal treatment as well as a additional treatment of meglumine adenosine cyclophosphate and Metoprolol.The clinical effect and changes of heart function will be evaluated in the two group with different treatment after three weeks,left ventricular ejection fraction and left nentricular volume at end-diastole,left nentricular volume at end-systolic were measured by M-mode.Echocardiography before and after curative in two group.Results Patients in the group with a combination application of meglumine adenosine cyclophosphate and metoprolol has a better clinical effect and a improved heart function than the control group.Conclusion A combination application of meglumine adenosine cyclophosphate and metoprolol has good curative effect in treating heart failure.

BACKGROUND:Bone marrow mesenchymal stem cells(BMSCs)are widely utilized as seed cells or carriers in bone tissue engineering and gene therapy.Thus,how to obtain BMSCs with high purity arose more attentions of researchers.OBJECTIVE:To observe the effects of different separation methods and culture conditions on biological characteristics of rabbit BMSCs.METHODS:BMSCs were obtained by whole bone marrow culture,density grand centrifugal and red blood cell lysis.At 48 hours after culture,the cell numbers were counted,the time of passage was recorded,in addition,the cell morphology was observed by phase contrast microscope,and the CD44 antigen expression was identified using flow cytometry.The 3rd and the 7th generation aging cells were cultured with DMEM-LG,MEM-HG,and DMEM/F12 culture medium.MTT and count cell plat were used to evaluate the growth of BMSCs.Phase contrast microscope was used to observe the morphological changes of aging cells.RESULTS AND CONCLUSION:BMSCs could be separated by each method.The adherent cells showed shuttle or multiple angle shapes,with rich cytoplasm,and positive for CD44 antigen.The more cell number and shortest primary culture time was presented in red blood cell lysis group(P ＜ 0.05).DMEM/F12 could promote the proliferation of quiescent cells.And the cells prevented the better viability.The method of read blood cell lysis improving the efficiency of BMSCs adherent is an effective method of extraction of BMSCs.DMEM/F12 could promote the proliferation Maybe,DMEM/F12 is more suitable for BMSCs.

Objective To assess the damage of secondary focal segmental glomerulosclerosis(sFSGS) in IgA nephropathy (IgAN). Methods Clinical and pathological data on 182 patients with IgAN were analysed quantitatively ,who had pathological damage of sFSGS.The clinical data included gender,age,blood pressure,urine protein,serum creatinine (Scr),blood urea nitrogen (Bun),uric acid (UA),blood cholesterol (Ch),triglycerides (TG),serum total protein (TP),albumin (Alb) and creatinine clearance rate (Ccr).The data of pathology included quantitive integration in focal and segmental glomerulosclerosis,cellular proliferation,mesangial proliferation,crescent,inflammatory cell infiltration,interstitial fibrosis,tubule atrophy and angio-lesion.The multivariate over univariate statistical analyses had been done,finding the correlation among the factors.Results The most common clinical type of 182 patients' was asymtomatic urine abnormality(50.55%),then were hypertension with/no chronic renal function failure and mass proteinura/nephrotic syndrome.The quantitive value of urine protein,Scr,Bun and UA increased as exacerbation of sFSGS' degree.It showed that inflammatory cell infiltration,interstitial fibrosis,tubule atrophy and angio-lesion had intimate correlation with sFSGS(r_1=0.377,r_2=0.169,r_3=0.412,r_4=0.331,P

Objective To observe the clinical effects of Bufei Yishen treatment on quality of life of patients with chronic obstructive pulmonary disease in stable period. Methods Sixty-four patients were randomly divided into two groups, the control group (32 cases) received Atrovent metered dose inhaling and Mucosolvan po. The treatment group (32 cases) received the fluid extract of Bufei Yishen besides the routine treatment. Both groups were treated for three months. The changes of scores of TCM syndrome and cardinal symptom, quality of life (QOL), lung function were observed before and after the treatment. Results The scores of TCM syndrome and cardinal symptom, quality of life of the treatment group were significantly higher than the control group (P0.05). Conclusion Bufei Yishen treatment has significantly effect on quality of life of patients with chronic obstructive pulmonary disease in stable period.

Objective To investigate the effect of Supplementing Qi and activating blood and tonifying kidney therapy on the function and quality of life in patients with prolapse of lumbar intervertebral disc after operation.Methods92 cases of lumbar disc herniation treated in Fuyang Hangzhou Hospital of traditional Chinese Medicine hospital from November 2015 to October 2016 were selected,divided into observation group and control group, there were 46 cases in each group, the observation group were treated with Supplementing Qi and activating blood and tonifying kidney therapy,the control group were given conventional western medicine treatment, the improvement of VAS score, JOA score and WHQOL-BREF score were compared between the two groups after treatment, and the clinical effects were compared between the two groups after treatment and the incidence of adverse reaction.ResultsAfter treatment,the VAS scores of the two groups were decreased, the observation group (1.85±0.43) is lower than the control group (2.97±0.69), the difference between the two groups was statistically significant(P<0.05);After treatment, patients withthe JOA scores of the two groups were increased, the observation group (25.78±5.89) higher than the control group (20.45±4.82), the difference between the two groups was statistically significant(P<0.05);After treatment, the total efficiency of observation group was 93.48% higher than 76.09% in the control group, the difference between the two groups was statistically significant(P<0.05);There was no significant difference in WHOQOL-BREF scores between the two groups before treatment;After treatment, the WHOQOL-BREF scores of the two groups were increased, the observation group was higher than the control group, the difference between the two groups was statistically significant(P<0.05), there was no significant difference in the incidence rate of complications between the two groups after treatment.ConclusionThe patients with lumbar disc herniation after operation to give Supplementing Qi and activating blood and tonifying kidney therapy,can effectively helpe patients to recover, improve the pain of patients, improve the patient's functional activities and quality of life, the effect is significant.

Objective: To detect estrogen receptor α and β(ER-α, ER-β)protein expression in different age of mouse thymus.Methods:Protein expression of ER-α and ER-β in thymus was analyzed via immunohistochemistry.Moreover,the relationship between ER-α and cytokeratin 18(epithelial cell marker)was further tested through fluorescence double-staining.Results: Immunohistochemical analysis revealed that both ER-α and β protein was found in nuclei of some thymocytes of 3 month mice.However,expression of ER-β was absence while ER-α was still positive in aged mice, such as 12 months and 16 months old.Double staining further confirmed that lots of ER-α/β positive cells were Foxp3 negative cells.Conclusion: Expression of ER-β is absent while ER-α is still positive in thymus of aged mice, which indicates ER-α is the critical estrogen receptor that involves in thymic involution.Moreover, ER-α/β do not participate in Treg development within thymus.

Objective:To detect estrogen receptor ? and ?(ER-?,ER-?) protein expression in different age of mouse thymus.Methods:Protein expression of ER-? and ER-? in thymus was analyzed via immunohistochemistry.Moreover,the relationship between ER-? and cytokeratin 18(epithelial cell marker) was further tested through fluorescence double-staining.Results:Immunohistochemical analysis revealed that both ER-? and ? protein was found in nuclei of some thymocytes of 3 month mice.However,expression of ER-? was absence while ER-? was still positive in aged mice,such as 12 months and 16 months old.Double staining further confirmed that lots of ER-?/? positive cells were Foxp3 negative cells.Conclusion:Expression of ER-? is absent while ER-? is still positive in thymus of aged mice,which indicates ER-? is the critical estrogen receptor that involves in thymic involution.Moreover,ER-?/? do not participate in Treg development within thymus.

Aim To explore the effect of nNOS specific inhibitor 7-nitrioindazole(7-NI) on the neurogenesis after focal cerebral ischemia in the adult mice dentate gyrus.Methods Focal cerebral ischemia was induced by middle cerebral artery occlusion(MCAO).A bromodeoxyuridine(BrdU) method was used to identify the proliferated cells in hippocampal dentate gyrus.Step-down test was performed to assay the learning and memory function.Results The number of BrdU~+ cells was increased in the ipsilateral but not contralateral dentate gyrus after focal cerebral ischemia.7-NI promoted the increment in the ipsilateral dentate gyrus significantly.7-NI also improved the survival of the new-born cells,as well as the learning and memory function of ischemic mice.Conclusion Our results indicate that nNOS down-regulates the neurogenesis after focal cerebral ischemia.

Objective To explore the effects of Sinomenine (Sin) on phenotype transdifferentiation in renal tubular epithelial cells. Methods Mice primary tubular epithelial cells and cell line (MCT) were cultured in vitro. Model group cells were stimulated by IL-1?(10 ng/ml). The cells of Sin group were co-incubated with Sin(10, 100 ,500?mol/L) simultaneously. The cells without IL-1?and Sin treatment were regarded as control group. The protein expression of?-smooth muscle actin (SMA) and Vimentin was assessed by immunohistochemistry and Western blotting. The mRNA expression of?-SMA and Vimentin was examined by RT-PCR. Secretion of matrix metalloproteinases was determined by zymography. Results The mRNA expression and protein level of?-SMA and Vimentin, and the enzyme activity of MMP-2 and MMP-9 in tubular epithelial cells stimulated with IL-1?were significantly higher than those in the controls. Sinomenine significantly reduced the expression levels of protein and mRNA of?-SMA and Vimentin. At the same time, the MMP-2 and MMP-9 activities were reduced markedly. Conclusion Sinomenine can decrease the transdifferentiation in renal tubular epithelial cells stimulated by inflammatory factor IL-1?and prevent renal interstitial fibrosis.

Objective To explore the mechanism of the inflammatory factors to increase the reanl inferstitial fibrosis by stndying on epithelial-to-mesenchymal transition induced by IL-1?.Methods Mice primary tubular epithelial cells were Cultured in vitro,then stimulated by IL-1? for 3,4,5 days respectively,from which the protein was extracted and the protein expression of ?-SMA and Vimentin was detected with western bloHing;while the cells were stimnlated by IL-1? for 12hs,36hs and 60hs respectively,from which then total RNA was extracted and the mRNA expression of ?-SMA and Vimentin was detected by ranscription-polymerase chain reaction analyses(RT-PCR).those without IL-1?stimulating were regarded as control group.Results The protein levels of ?-SMA,Vimentin in tubular epithelial cells stimulated with IL-1?for 3 days or 4 days were significantly higher than that in normal group cells.Those stimulated for 5 days,the protein levels of ?-SMA,Vimentin in tubular epithelial cells increased very high.The mRNA expression of ?-SMA and Vimentin increased significantly in 12hs in tubular epithelial cells after IL-1?stimulating as compared with normal group cells and presented time-depend.Conclusions IL-1? plays the role of promoting renal interstitial fibrosis by inducing the transdifferentiation of renal tubular epithelial cells and reducing the proliferation cells probably.