Objective To investigate human esophageal cancer Eca109 cells apoptosis induced by resveratrol.Methods Eca109 cells were treated with difierent concentrations of resveratrol and the change of morphology was observed by inverted microscope and transmission electron microscope in different time;The cell growth inhibitory rate of resveratrol on the proliferation of Eca109 cells were evaluated in vitro by MTT assay.Flow cytometry (FCM)analysis was used to determine the apoptosis of Eca-109 cells.Results Resveratrol was able to inhibit the growth of Eca109 cells,and the suppressive effect was increased with treating concentration and treating time.Resveratrol inhibited the growth of esophageal cancer Eca109 cells in a dose-and time-dependent manner and induced the apoptosis in esophageal cancer.Conclusion Resveratrol could inhibit the proliferation of Eca-109 cells and block the cell cycle at G0/G1.And in the end resveratrol could induce the apoptosis of Eca109 cells.

Objective To observe the clinical efficacy of deep needling at Tianshu (ST25) with electroacupuncture in treating post-stroke constipation. Method Forty-eight patients with post-stroke constipation were randomized into an electroacupuncture (EA) group and a medication group, 24 cases in each group. The EA group was intervened by deep needling at Tianshu with EA, while the medication group was by Cong Rong Tong Bian oral liquid. After 1 treatment course, the constipation symptom score was observed and the clinical efficacy was calculated. Result After 1 treatment course, the symptom score, total effective rate, recovery plus markedly-effective rate of the EA group were superior to that of the control group, and the inter-group differences were statistically significant (P<0.05). Conclusion Deep needling at Tianshu with EA can significantly enhance the clinical efficacy in treating post-stroke constipation and improve the quality of life.

Objective To investigate the risk factors and effective nursing for patients with benign prostatic hyperplasia(BPH)within 3 days after operation.Methods The risk factors for restlessness of patients with BPH in 2006 were analyzed and the nursing points were summarized.Results Age of patients,surgical style and sleeping time before the operation were evidently related to the incidence of restlessness.Conclusion Strengthening of psychological nursing,appropriate surgical style and insurance of abundant sleeping time before operation could reduce the incidence of restlessness of patients after operation.

From October 1978 through 1983, 225 rectal cancer patients were randomized into R+S group (115 patients) and S only group (110 patients). The radiation dose of pre-operative radiotherapy was 30～45Gy/3～4.5 wks. Radical or palliative resection was done after a rest of 2～4 weeks. The lymphatic metastasis and local recurrence rates of the R+S group (20.6% and 27.8%) were lower than those of the S only group(32.7% and 41.8%)(P

OBJECTIVE: To study the effect of insulin injection on the stability of cefodizime sodium for injection in 5% glucose injection.METHODS: The appearance and the pH value of the mixed solution in 12 h were monitored and the content of cefodizime sodium in the mixed solution was determined by HPLC.RESULTS: There were no evident changes for the mixture in appearance,pH value and the content of cefodizime sodium within 12 h at room temperature.CONCLUSION: Insulin injection has no effect on the stability of cefodizime sodium within 12 h at room temperature.

Objective To investigate the clinical effects,feasibility,points for attention and complications of treating distal radial fractures through minimally invasive palmar locking plate.Methods A total of 22 patients with distal radial fractures managed by minimally invasive palmar locking plate from August 2009 to August 2010 were enrolled in the study.According to AO classification,there were five patients with type A2 fractures,three with type A3,four with type B1,seven with type B3,two with type C1 and one with type C2.Two vertical or parallel palmar incisions of 2 cm long were performed.The distal transverse incision was along the proximal wrist crease,while the distal and proximal vertical incisions were close to the radial side of flexor carpi radialis.Then,palmar locking plate was inserted through deep pronator quadratus.Results Follow-up period was 10-18 months(mean,12 months).According to Dienst function evaluation criteria,the results were excellent in 13 patients,good in six,fair in three and poor in zero,with excellence rate of 86％.Complications were as follows:one patient had delayed healing of proximal wrist crease incision;one patient had numbness of thenar eminence and the symptom was disappeared three months postoperatively;two patients remained pain on ulnar side of the wrist joint;two patients had limited function of the wrist joint.Conclusions Minimally invasive palmar locking plate is safe,mini-invasive and reliable when treating distal radial fractures,which is conducive to early functional exercise.Furthermore,the method achieves satisfactory appearance of the incision which meets the minimal invasion and thus deserves clinical practice.

AIM: To extract and isolate polysaccharide from Poacynum Hendersonii leaves,determine its content and analyze the monosaccharide composition.METHODS: Poacynum Hendersonii leaves was extracted with hot water,crude polysaccharide was precipitated with ethanol,deproteinated according to Sevage method,coloured with acticarbon.Then of polysaccharide contents were measured by anthrone-H_2SO_4 colorimetry at the wavelength of 620 nm.The monosaccharide composition was determined by HPCE.RESULTS: The polysaccharide content was 0.97% of leaf weight,and Gal,Ara,and Man contents were three higher monosaccharides.CONCLUSION : The method is easy to carry out the baseline resolution in HPCE and has highly sensitivity.

Objective To investigate the effect of orphan nuclear receptor NR4A1 expression on oxygenglucose deprivation (OGD)-induced apoptosis in cultured rat cerebellar granule neurons and its possible mechanisms.Methods Primary rat cerebellar granule neurons were cultured for 7 to 8 days,and then treated with OGD.The activity of cultured rat cerebellar granule neurons was assessed by methyl thiazolyl tetrazolium (MTT) assay,apoptosis was detected with flow cytometry,the expressions of NR4A1,caspase-3 and cytochrome c were determined by Western blot analysis,NR4A1 mRNA expression was detected with real-time polymerase chain reaction.The rat cerebellar granule neurons were transfected with lentiviral vector-encoding rat NR4A1.The apoptotic rates and expressions of caspase-3 and cytochrome c in rat cerebellar granule neurons transfected with NR4A1 were detected after OGD.Results The activity of rat cerebellar granule neurons decreased significantly,the apoptotic rate increased significantly,the expressions of NR4A1 mRNA and protein as well as caspase-3 and cytochrome c incrased significantly along with the OGD time.NR4A1 was overexpressed,apoptosis rate was significantly reduced in rat cerebellar granule neurons transfected with NR4A1.The expressions of caspase-3 and cytochrome c were significantly reduced in the rat cerebellar granule neurons transfected with NR4A1 after OGD.Conclusions NR4A1 overexpression may reduce OGD-induced apoptosis in rat cerebellar granule neurons by downregulating the expressions of caspase-3 and cytochrome c.

90%inasinglestep,andtheproteinyieldwas2.5mg/L.ConclusionPurifiedChsp60kDantigenisobtained,andtheantigenmightbeappliedtodetectantibodyinpatientsinfectedwithChlamydialtrachomatis,andwillcontributetostudytheroleofChsp60inimmunopathogenesis.

Aim To explore the effect of nNOS specific inhibitor 7-nitrioindazole(7-NI) on the neurogenesis after focal cerebral ischemia in the adult mice dentate gyrus.Methods Focal cerebral ischemia was induced by middle cerebral artery occlusion(MCAO).A bromodeoxyuridine(BrdU) method was used to identify the proliferated cells in hippocampal dentate gyrus.Step-down test was performed to assay the learning and memory function.Results The number of BrdU~+ cells was increased in the ipsilateral but not contralateral dentate gyrus after focal cerebral ischemia.7-NI promoted the increment in the ipsilateral dentate gyrus significantly.7-NI also improved the survival of the new-born cells,as well as the learning and memory function of ischemic mice.Conclusion Our results indicate that nNOS down-regulates the neurogenesis after focal cerebral ischemia.