Objective To establish a method of HPLC for determining the Paeonol in Shengui Tiaojing Pill. Method Agilent Hypersil-ODS-C18 (250 mm?4.0 mm, 5 ?m) was used, the mobile phase consisted of methanol-H2O (70∶30). The flow rate was 1.0 mL/min. The detecting wave length was at 274 nm. Results The method was linear at the range of 0.052～0.84 ?g for Paeonol (r=0.999 9). The average recovery was 99.94%. Conclusion The method is simple, sensitive, accurate and can be applied to the quality control of Paeonol in Shengui Tiaojing Pill.

Objective To investigate the pollution status of heating-ventilating-air conditioning(HVAC) systems in Shenyang public place. Methods The dust samples were collected and examined from the HVAC system in twelve hotels and ten shopping malls in Shenyang in March-June, 2004. Results Among HVAC systems in Shenyang public buildings, 71.51% of them were moderately polluted and 27.37% were seriously polluted. The rate of serious pollution of hotels was higher than that of shopping malls, the volume of dust in the air pipes in hotels was lager, but the amount of bacteria and fungi was less than that of shopping malls. Conclusion The HVAC systems of Shenyang public buildings have been seriously polluted.

Objective To investigate the influence of tiotropium bromide on lung function index,quality of life and re-hospitalization risk of patients with bronchial dilation and pulmonary infection.Methods 100 patients with bronchiectasis and lung infection were chosen,and they were randomly divided into control group (50 patients) with BAL used alone and observation group(50 patients) with tiotropium bromide on the basis of control group.The short-term clinical effects,the levels of FVC,FEV1 and FEV1 ％,BODE index scores and QLI scores before and after treatment,and re-hospitalization rate of the two groups were compared.Results The short-term effective rate of the observation group was significantly higher than that of the control group(94.00％ vs.76.00％,x2 =7.81,P ＜ 0.05).The levels of FVC,FEV1 and FEV1％ after treatment of both two groups were significantly higherthan before treatment[(2.78 ±0.57)L vs.(2.15 ± 0.33) L,(3.60 ± 0.71) L vs.(2.20 ± 0.36) L;(2.15 ± 0.42)L vs.(1.23 ±0.32)L,(2.77±0.59)L vs.(1.17 ±0.29)L;(65.25 ±11.45)％ vs.(51.79 ±9.23)％,(77.46 ±15.80) ％ vs.(52.02 ± 9.36) ％,t =2.34,2.97;2.52,3.07;2.47,3.12,all P ＜ 0.05].After treatment,the levels of FVC,FEV1 and FEV1 ％ of the observation group were significantly higher than control group [(3.60 ± 0.71) L vs.(2.78 ±0.57)L;(2.77 ±0.59)L vs.(2.15 ±0.42)L;(77.46±15.80)％ vs.(65.25 ± 11.45)％,t =2.26,2.44,2.30,all P ＜0.05].The QLI score and BODE index scores after treatment of both two groups were significantly better than before treatment[(3.37 ± 0.54) points vs.(3.95 ± 0.65) points,(2.11 ± 0.40) points vs.(3.87 ±0.61)points,(7.28 ±1.18)points vs.(5.04 ± 0.95) points,(8.69 ± 1.54) points vs.(5.13 ±0.98) points,t =2.29,2.81;2.50,2.96,all P ＜ 0.05].After treatment,the QLI score and BODE index scores of the observation group were significantly better than the control group[(2.11 ±0.40) points vs.(3.37 ±0.54) points,(8.69 ± 1.54) points vs.(7.28 ± 1.18)points,t =2.21,2.44,all P ＜ 0.05].The re-hospitalization rate of the observation group was significantly lower than that of the control group (16.00％ vs.2.00％;28.00％ vs.6.00％;x2 =8.02,9.74,all P ＜ 0.05).Conclusion Selective cholinergic receptor blocker combined with BAL in the treatment of patients with bronchial dilation and pulmonary infection can efficiently relieve the respiratory symptoms,improve lung function,improve the quality of life and is helpful to avoid re-hospitalization occurrence.

Objective To explore the effects of hyperbaric oxygen(HBO) on brain derived neurotrophic factor(BDNF) and the changes of neuron structure after cerebral ischemia-reperfusion(CIR) in mice. Methods Twenty-seven mice were randomly and evenly divided into three groups : a HBO group, a control group and a sham-operation group (SO group). CIR models were established by clamping both carotid arteries in mice for 30 min,and then, untying clamps. 0.20 MPa HBO was applied once a day for 10 days after establishment of models in HBO group,but not in the control and SO groups. Following sacrifice after treatment and acquirement of cortex and hippocamal tissues, HE stain and immunohistochemistry technique were used to observe the changes of neuron structure and expression of BDNF. Results The neural degeneration and necrosis in cortex and hippocampus (issues) were observed under light microscope after CIR, and the amount of abnormal cells in the control group was more than that in HBO group (P

Objective To study the value of oxygen treatment on elderly patients with acute ischemic stroke.Methods From April 2010 to March 2012,a total of 140 cases of elderly patients with acute ischemic stroke for oxygen therapy combined with drug and rehabilitation were divided into two groups,outside high-flow oxygen group of 71 cases,hyperbaric oxygen group of 69 cases.The neurological functional defects scale was used to evaluate the effect.Two groups of patients were given conventional drug therapy including improving the microcirculation,vegetative neural drugs,and rehabilitation treatment.Results The total effectiveness respectively was 98.6％ (70/71),91.3％ (63/69),and there was significant difference (P ＜ 0.05).After treatment,the nerve function damage grades of two groups respectively was (10.4 ± 1.7),(15.6 ± 1.8) scores,and there was significant difference compared with that before treatment [(28.2 ± 2.5),(28.7 ± 2.6) scores] (P ＜ 0.05).There was significant difference in treatment adherence between two groups (P ＜ 0.05).Conclusion For patients of elderly patients with acute ischemic stroke,oxygen therapy is necessary,while outside high-flow oxygen therapy is better than hyperbaric oxygen therapy.

Objective To explore the surgery way of anterolateral small incision total hip replacement and evaluate the curative effect after surgery.Methods Clinical data of 41 patients(48 hips)with anterolateral small incision total hip replacement were analyzed retrospectively.The incision length,operation time,intraoperative blood loss,postoperative volume of drainage,perioperative complications,hospitalization days,X -ray performance were recorded.Results The incision length was 7-8cm,mean (7.5 ±0.5)cm.The operation time was 60-70min,mean (65 ±5)min.The intraoperative blood loss was 165 -280mL,mean (235 ±44)mL and the postoperative volume of drainage was 85 -120mL,mean (95 ±15)mL.No perioperative complications occurred.The average follow-up time was (36 ±6)months.The preoperative hip joint Harris score was (30.3 ±28.2)points,and the last follow-up score was (98.0 ±4.0)points,the difference was statistically significant(t=15.665,P=0.000),and the excellent and good rate was 100%.Conclusion The anterolateral small incision total hip replacement has small trauma,less bleed-ing,less postoperative pain,quick recovery,better joint stability,and it is suitable for clinical promotion.

Objective To evaluate the efficacy of preoperative regional intra-arterial chemotherapy (RIAC) in the treatment of resectable pancreatic head carcinoma. Methods The clinical data of 50 patients with resectable pancreatic head carcinoma who had been admitted to the Research Institute of Pancreatic Diseases of Fudan University from December 2006 to July 2007 were retrospectively analyzed. Patients were randomly divided into2 groups (n =25 in each group): patients in group A were treated with preoperative RIAC followed by regional pancreaticoduodenectomy, and patients in group B were treated with surgical procedure routinely. The lymphatic metastases in the 50 specimens of pancreatic head carcinoma were detected by histological examination with hematoxylin and eosin (HE) staining, and lymphatic micrometastases were detected by immunohistochemical method with staining of cytokeratin AE1/AE3 in 10 specimens with negative HE staining of the lymph nodes in each group. Results There was no significant difference in the incidence of complications, the length of hospital stay and the 1-, 2-year survival rates between the 2 groups (χ2 = 0.12, 2.88, P > 0.05). The incidence of positive lymph node metastasis in group A was 7.1% (52/734), which was significantly higher than 22.1% (118/532) in group B (χ2 = 60.01, P < 0.05). The incidence of lymphatic micrometastasis was 9.4% (30/319) in group A, and 9.1% (23/252) in group B, with no statistical difference between the 2 groups (χ2= 0.01, P > 0.05). Conclusions Preoperative RIAC is helpful in improving the prognosis of patients with resectable pancreatic head carcinoma by reducing the incidence of lymphatic metastasis and decreasing tumor stage.

Objective The purpose of the present study was to investigate the effect of peroxisome proliferator-activated receptors δ（PPAR δ）activation with dietary GW610742X on the expression of tenascin-C in the infarcted and remodeling myocardium.Methods Sixty male Wistar rats were divided into four groups,including control group,sham group,myocardial infarction（MI） group,and MI＋GW610742X（GW）group.The left coronary artery was ligated to establish the MI model.PPAR δ activator GW610742X（100mg/kg/d）was administrated into the rats of GW group.At 3 months after procedure,the expression and distribution of tenascin-C in left ventricular free wall from each group were examined by Western blotting and immunofluorescence,respectively.Results After 3 months following procedure,there were obvious necrosis and fibrosis in left ventricular free wall from MI group.The expression of tenascin-C in MI and GW group was significantly higher than those in control and sham group（P 〈 0.01）.Moreover,tenascin-C expression in GW group was remarkably decreased compared to MI group（P 〈 0.05）.Additionally,tenascin-C expression in sham group was similar to that in control group（P 〉 0.05）.Conclusion The tenascin-C is upregulated in infarcted myocardium during the remodeling process,which can be significantly attenuated by PPAR δ activation.

AIM:To investigate NO-donor sodium nitroprusside(SNP)-induced apoptosis of human airway smooth muscle cells(HASMCs) of passive sensitization by serum from allergic asthmatic patients.METHODS: The technique of human airway smooth muscle(HASMCs) passively sensitized with serum from allergic asthmatic patients was adopted.The effect of SNP on the survival rate of passively sensitized HASMCs was detected by MTT method.Apoptosis of cells was detected by TUNEL and flow cytometry.RESULTS:(1) Compared to sensitized group,the survival rate of passively sensitized HASMCs decreased in SNP+sensitized group(n=5,P

Objective To survey the frequency of H deficient phenotype in blood donor population and analyze the serological and genetic characteristics of these individuals.Methods The H deficient phenotype was screened with anti-H monoclonal antibody.The ABO type was screened with serological method and with sequence specific primer polymerase chain reaction(PCR-SSP).FUT1 and FUT2 gene sequences were analyzed with direct sequencing of PCR products and gene cloning products.Result Of 85 390 blood donors,ten individuals were identified to be para-Bombay phenotype.Four h alleles were found in 14 para-Bombay phenotype individuals,h1(nt547-552?ag),h2(nt880-882?tt),h3(nt658c→t),and h_(new-2)(nt328g→a).The FUT1 genotypes of these para-Bombay individuals were h1/h1(6 individuals),h1/h2(7 individuals) and h3/h_(new2)(1 individual),and the frequency of 4 allele were 67.85%(h1),25%(h2),3.57%(h3),and 3.57%(h_(new-2)),respectively.FUT2 gene was analyzed in 12 para-Bombay phenotype individuals,and a mutation of nt357c→t was detected in all FUT2 gene,another mutation of nt716g→a were heterozygous in 5 individuals with h1/h2 genotype.No null FUT2 gene was detected.In serological analysis,all atypical anti-A or anti-B antibody of 14 para-Bombay individuals were inactive at 37℃,7 individuals had active anti-H antibody at 37℃.Conclusion The frequency of H deficient phenotype in Fujian population is about 1:8 500.The h1 and h2 alleles are predominant in Fujian H deficient individuals on h1-Se~(357) and h2-Se~(357,716) haplotype background.