Evidence-based anesthesia’s core thinking is to abide by evidence,which means clinical decision must be based on objective research result,personal experiences and patients` actual situation. The thinking of EBA will magnificently promote the conversion of model of clinical anesthediology education,and will make significant influence on clinical teaching,scientific research, and clinical practice. According to the current situation,try to give out new teaching model of evidence-based practice of anesthesiology and offer teaching methods and practical procedures.

Objective: To explore characteristics and influencing factors for blood pressure variability (BPV) in aged patients with H type hypertension.Methods: A total of 100 patients with essential hypertension, who were treated in our hospital from Dec 2012 to Dec 2013, were selected.According to serum level of homocysteine (Hcy), patients were divided into pure hypertension group (n=42, Hcy<15.4 μmol/L) and H type hypertension group (n=58, Hcy≥15.4 μmol/L).All patients received 24h ambulatory blood pressure monitoring (24hABPM), and BPV indexes were recorded and compared between two groups.Correlation among BPV indexes and general data were analyzed, and multi-factor linear regression analysis was used to analyze influencing factors for BPV indexes.Results: Compared with pure hypertension group, there were significant rise in 24h systolic blood pressure standard deviation [24hSSD, (12.80±2.23) vs.(16.01±3.11)], daytime SSD [dSSD, (11.58±2.30) vs.(13.86±2.26)] and nighttime SSD [nSSD, (9.41±1.64) vs.(11.71±2.34)] in H type hypertension group, P<0.05 or <0.01.Pearson correlation analysis indicated that Hcy, total cholesterol (TC) and low density lipoprotein cholesterol (LDL-C) were significantly positively correlated with 24SSD, dSSD and nSSD (r=0.201~0.508, P<0.05 or <0.01).Multivariate linear regression analysis indicated that Hcy and TC were significantly positively correlated with 24SSD, dSSD and nSSD (B=0.194~0.546, P<0.05 or <0.01).Conclusion: Systolic blood pressure variability in aged patients with H type hypertension is significantly higher than that of patients with pure hypertension,their serum Hcy level, TC elevating level are significantly positively correlated with systolic blood pressure variability and are major influencing factors for BPV in these patients.

ObjectiveTo assess the impact of nutrition education on the outcomes and dietary intake during pregnancy.MethodsFrom January 2007 to December 2008,240 healthy pregnant outpatients were selected from Jining First People's Hospital.Subjects were randomly and equally divided into the intervention group and control group,and the intervention group received nutrition education till labor.Through the comparison of two groups,the impact of nutrition education on the outcomes and dietary intake was assessed.ResultsBirth weight of the newborn infauts was significantly lower in the intervention group than the control group( (3.15 ±0.81 )kg vs (3.33 ± 0.56)kg,t =2.04,P ＜ 0.05).Compared with the control group,the incidence rate of anemia was lower in the intervention group (45.8％ vs 59.8％,x2 =4.56,P ＜0.05).The intervention group had higher proportion of supplementation in iron,folic acid and compound vitamins (P ＜ 0.05).Significant differences were also observed in the frequency of intake fried food,pork,poultry,milk,soybean milk and fresh fruits (P ＜ 0.05).ConclusionNutrition education can improve the outcomes and dietary intake during pregnancy.

Objective To analyze and compare the HBV DNA contents in serum and breast milk after injection of hepatitis B im‐munoglobulin (HBIG) in different periods of pregnant and lying‐in women to provide the experimental basis for blocking the mater‐nal‐neonatal transmission(PMTCT) and breast feeding scheme .Methods 140 pregnant women carrying hepatitis B virus with HB‐sAg(+ ) by antenatal examination in the obstetric outpatient department of our hospital from June 2012 to June 2014 were selected and divided into the research group and the control group according to the voluntary and secretive principle .Among them ,75 cases in the research group were intramuscularly injected by high titer HBIG 200 U at 28 ,32 ,36 weeks of pregnancy ,while 65 cases in the control group were injected by HBIG at the end of pregnancy due to different causes .Serum HBV‐DNA content before injection and before delivery was detected in the two groups ,and which in neonatal serum and breast milk within 3-5 d also detected .The differences and correlation between the two groups were analyzed .Results The HBV‐DNA content <500 copies/mL ,500-1 × 106 copies/mL ,>1 × 106 copies/mL before HBIG injection in the research group were 28 cases ,17 cases ,30 cases respectively ,which before delivery were 35 cases ,20 cases ,20 cases respectively ;which in antenatal twice detection in the control group were 19 cases , 21 cases ,25 cases and 20 cases ,17 cases ,28 cases respectively ;neonatal serum HBV‐DNA positive in the research group and control group had 1 case(5 .3% ) and 5 cases (7 .7% ) respectively ;the breast milk HBV‐DNA positive in the two groups had 3 cases(4% )/and 8 cases(12 .3% ) respectively .Conclusion HBIG injection at late pregnancy in the pregnant women carrying HBV could influ‐ence the HBV replication ,thus reduces the probability of neonatal intrauterine infection ,at the same time reduces the HBV‐DNA positive rate of postpartum breast milk .

Objective To investigate the effects of ischemia reperfusion on the morphological changes of sinoatrial node(SAN) cells in rabbits in vivo . Methods Ninety healthy adult rabbits were randomly divided into 3 groups with 10 rabbits in control and every subgroup: control group [a suture passed under the root section of right coronary artery(RCA) without ligation], ischemia group(occluding the root section of RCA for 10, 30, 60 and 120 min respectively), ischemia reperfusion group(10, 30, 60 and 120 min ischemia after respectively followed by 4 hours′ reperfusion). Results ① Compared with control group, abnormal structure of SAN cells was not seen in 10 min ischemia group and 10 min ischemia reperfusion group with light microscopy, except slight mitochondrial swelling of SAN cells in former group with electron microscopy. ② There were morphological changes of SAN cells in 7, 6 and 8 rabbits in 30, 60 and 120 min ischemia group, presenting mainly as cellular swelling, karyopyknosis, and focal necrosis under a light microscope, and mitochodrial swelling, cristae disorganization or break under an electron microscope. The most severe cellular damages were found in 120 min ischemia group. ③ In ischemia reperfusion group, there were morphological abnormalities of SAN cells in 6, 8 and 7 rabbits in 30, 60 and 120 min ischemia reperfusion groups. The morphologic changes were similar to those in ischemia group, but injury degree was more severe than that in ischemia group with the same ischemic time. Conclusion Ischemia and ischemia reperfusion can induce morphological changes of SAN cells in a time dependent manner in rabbits in vivo , and the injury degree is more severe in ischemia reperfusion group than that in ischemia group with the same ischemic time.

60 years senile autopsy cases w as about half of that of western countries,most of latent carcinomas were well a nd moderate differentiated.HGPIN was closely associated with prostatic adenocar cinoma.

Objective To study the relationship between the methylation of p16 promotor and the pathogenesis and progress of psoriasis. Method Methylation-specific PCR and DNA sequencing were used to detect the methylation of p16 promotor. Results The methylation of p16 promotor was found in 23.08% (6/26) of psoriatic lesions and 19.23% (5/26) of non-lesional areas in psoriatic patients, but none in normal controls. The frequency of methylation of p16 promotor was higher in psoriatic lesions than that in normal skin (P

Objective Use McGurk effect paradigm materials to explore the role of lip-Reading for hearing-impaired children in phonetic identification.Methods Thirty-six severe prelingually hearing-impaired children and thirty-six normal healthy children(binaural PTA≤20 dB HL)were recruited in the study.The test material was composed of audio and video,including/ba/,/da/,/bi/,/di/,/bu/,/du/six stimulation sounds which were video recorded as pronounced.Under 3 different conditions,the two groups were evaluated for the accuracy of audi-tory responses.Results The accuracy of auditory responses of hearing-impaired children group was 0.71 ±0.19, 0.96±0.07,0.11 ±0.16 for auditory-only mode,audio -visual consistent mode and audio -visual inconsistent mode,respectively,0.93±0.10,0.96±0.11,0.54±0.23 for normal hearing children in auditory-only mode,audi-o-visual consistent mode and audio-visual inconsistent mode,respectively.Conclusion The Audio-visual con-sistent mode is the best one and the lip-reading can help hearing-impaired children in phonetic identification.

[Objective] To compare and analyse the clinical effect of tiny incision blind-cut suck-erasion on unhairing and preserving armpit hair in bromhidrosis.[Method] Select 429 cases of 851 sides in our hospital, divide them into treatment group(blind-cut suck-erasion+unhairing) 308 cases with 609 sides, and control group(blind-cut suck-erasion+preserving armpit hair) 121 cases with 242 sides. Make statistics and compare their complications in recent and forward stages, also the recurrence and residual of peculiar smel after 1 year. [Result] For the treatment group, 38 sides had the recent compli-cation of hematoncus, 30 sides had partly skin necrosis, 12 had bad cut cure, 70 had early slight hardening and contracture skin; for the forward complica-tions: 27 sides had focal slight scar, 3 severe scar, 23 focal subcutaneous sac lump with infection, 3 peculiar smel residual and recurrence. To the control one, for the recent complications, 11 sides had hematoncus, 9 partly skin necrosis, 5 bad cut cure, 13 early slight hardening and contracture skin; for for-ward complications, 8 sides had focal slight scar, 1 severe scar, 3 focal subcutaneous sac lump with infection, 38 peculiar smel residual and recurrence. By comparison, on early slight hardening and contracture skin and focal subcutaneous sac lump with infection, the treatment group was higher than control one;other complications had no difference of statistical meaning;on radical cure rate, the treatment group was obviously more than control one, with dif-ference of statistical meaning. [Conclusion] The tiny cut blind-cut suck-erasion is definite in treating bromhidrosis, the operation removes subcutaneous apocrine sweat gland, meanwhile removes the hair fol icle and sebaceous gland complexus, the therapy is very thorough, with better cure effect, less recur-rence, without increasing complications.

AIM: To determine the HPLC fingerprint of ultramicro decoction piece of Radix paeoniae Alba. METHODS: HPLC was used to analyze the extracts of ultramicro decoction piece of Radix paeomae Alba from 10 different sources. RESULTS: The fingerprint of ultramicro decoction piece of Radix paeoniae Alba was composed of 20 peaks,among which there were 10 characteristic peaks. CONCLUSION: The fingerprmt can be used to control the ultramicro decoction piece of Radix paeoniae Alba qualities.