Objectives:To investigate the health state of civil servants in Chongqing.Methods:Divide the objects into three age-groups according to the WHO standard and analysis the healthy index including BMI,blood pressure,blood lipid,blood sugar,blood uric acid,antigens and antibodies of hepatitis B virus,abdomen B-ultrasound and so on,then to compare the state of health index in different age and gender.Result:High triglyceride,over weight and obesity,hypertension were the front third diseases which detected from the 13,380 health examination officers,the detection rate was 35.76%,33.54%,28.71%;Compare men with women,metabolic syndrome,hypertension,hyperuricemia,HBV carry rat in men were higher than in women(P

Objective:To study the health condition of persons with high professional title in Chongqing city.Methods:1,100 persons with high professional title in Chongqing received physical examination.The routine checking departments involved include: the department of internal medicine,surgery,ENT department,department of stomatology and gynecology,and the routine items include:biochemistry,X-ray,electrocardiogram,ultrasound et al.Results:1078 of 1100 examined persons (98%) had different degree of health problems.The first three common diseases are hypertension (28.18%),hyperlipemia (24.55%) and fatty liver (20%).3 cases of early malignancy (1 case hepatic carcinoma,2 cases of kidney tumor) were also found.Conclusion:The health condition of persons with high professional title in Chongqing city is not very good.It is urgent to release stress of work and life,improve diet structure,and strengthen exercises and health care.

Objective; To investigate the health condition of breast and gynaecology in one university female staff. Method; The breast condition was estimated by mammary gland surgery and mammary gland ultra - red ray , and gynae condition was estimated by gynaeco-logic routine examination, and cervical smear in 586 female staffs of a university. Results; 396 of 586 (68. 72% ) female staffs suffered gynaecologic and breast disease to different degrees. The first four leading disease were chronic cervicitis (32.22% ), colpitis (30.81% ) , hysteromyoma (5. 81% ) and breast hyperplasia (4.75% ). The occurrence of cervicitis in young females was higher than that in the middle aged and aged ( P

Objective To discuss the clinical efficacy of warm acupuncture, acupoint injection, bloodletting and electroacupuncture, and their interaction effects for acute cold type facial paralysis. Methods Totally of 48 patients with acute cold type facial paralysis were randomly divided into three groups by orthogonal experimental design, and then were assigned to 16 treatment plans. All patients received routine basic treatment, and then were given warm acupuncture, acupoint injection, bloodletting and electroacupuncture according to assigning results. 10 d were as a treatment course, with 2 d as interval, 3 treatment courses in total. The neurological function scores before and after treatment and facial nerve recovery time were recorded. Results There was statistical significance in scores of facial nerve function, warm acupuncture, acupoint injection, electroacupuncture, bloodletting had significant difference with errorterm (P<0.01). There was statistical significance in the recovery time of the facial nerve function, warm acupuncture, bloodletting compared with error term (P<0.01). Conclusion Warm acupuncture, acupoint injection, bloodletting and electroacupuncture combine with acupuncture are better for acute cold type facial paralysis. Warm acupuncture and bloodletting therapy in the acute phase of interventional therapy can shorten the recovery time of facial nerve.

Objective To discuss the relationship between IL-10 genes and susceptibility of H.pylori-associated gastric cancer of Han population in Chongqing district.Methods 100 H.pylori-positive patients with gastric cancer(gastric cancer group)and 140 H.pylori-positive healthy people (control group)were chose in the study,PCR-RFLP method was used to detect the IL-10-592 lo-cus polymorphisms of Han population in Chongqing district.Results Comparing with control group,IL-10-592 genotype frequency distribution of gastric cancer group has statistically significance (χ2=16.36,P<0.05).Logistic regression analysis showed that gastric cancer incidence risk of people carrying with IL-10-592 A/A genotype was OR=4.37(95%CI:2.04-9.38),comparing with the subjects carrying with IL-10-592 C/C.Conclusion Polymorphism of IL-10 gene has close correlated with H.pylori-asso-ciated gastric cancer,IL-10-592 C/C might be the susceptibility gene of H.pylori-associated gastric cancer.

The article introduces a set of efficient English teaching methods through continuous practice and reform.The methods are based on the characteristics of the foreign students.It includes choosing the book in English and compiling English-Chinese bilingualism textbook,question-based and quiz-based teaching in class and reviewing after class and so on.The methods have shown good effects in raising quality of teaching to the international students.At the same time there are some problems in the Lemology teaching.

Objective To study the relationship between the gene mutations of DNA gyrase subunit A(gyrA)and quinolone resistance in Salmonella typhi. Methods The genes of gyrA DNA of Salmonella typhi S275(a clinically isolated quinolone susceptible strain)and its spontaneous quinolone-re-sistant mutant RGl were examined in this study with polymerase chain reaction(PCR),restrictive frag-ments length polymorphism(RFLP),single strand conformational polymorphism(SSCP)and nucleotide sequencing. Results Nudeotide sequencing of gyrA in Salmonella typhi S275 revealed that the bases of 128～426 kept highly conservative as compared with those of Escherichia coli KL-16,with only 7.49%difference in the gyrA nucleotides 128～426 between the two strains.Most of the mutations were silent mutations,which contributed to 3 amino acid substitutions in gyrase(including Thr-45→His,Arg-49→Leu and Val-56→Gly),and all these substitutions were located outside the quinolone resistance determining re-gion(amino acids 67-106 of subunit A of gyrase).In comparison with Salmonella typhi S275,a single mutation was found at base 247 of gyrA of Salmonella typhi RG1,with change transferred from T to G and led to a substitution of Ser-83→Ala.The mutation might be responsible for the increase of MICs of nalidixic acid,ofloxacin and ciprofloxacin against Salmonella typhi from 2,0.06 and<0.03 to 512,2,and 1 mg/L respectively.Ser-83→A1a was also a newly discovered substitution in gyrA of Salmonella spp.The results of PCR-RFLP and SSCP were in concordance with results of nucleotide sequencing. Conclu.sions The mutation of gyrase at the 83rd amino acid maybe play a principal role in the resistance of Salmonella typhi to quinolone.

The initiation of puberty is associated with the hypothalamus-pituitary-gonadal( HPG) axis. The activation of gonadotropin-releasing hormone( GnRH) is the key factor in the initiation of puberty. The initi-ation of puberty is a complicated process,GnRH is under the influence of many associated neuropeptides. Re-cently,the studies have found that GnIH and Kisspeptin can respectively inhibit and promote the hypothalamus GnRH secretion of mammals,indicating that GnIH and Kisspeptin on regulation of reproductive endocrine axis play very important roles. Therefore,GnIH/GPR147 and Kisspeptin/GPR54 pathways may be closely associated with the initiation of puberty.

Airway remodeling is one of the main pathological changes of bronchial asthma. It has shown that a variety of cytokines, including chemokines, growth factors, matrix proteins, participate in and even strengthen airway remodeling through building complex signal transduction pathways. Many traditional Chinese medicines,such as shegan mahuang decoction,have a regulatory role in airway remodeling.