Objective To investigate the apoptosis induction of Bullatacin on A549 cell line of pulmonary adenocarcinoma. Methods The MTT assay was used to detect the growth inhibition rates of A549 cells cultured with Bullatacin in different concentrations (6.25, 12.5, 25, 50, 100μg/mL). 25μg/mL Bullatacin was used to culture A549 cells for 0, 12, 24, 48 h. The cell cycle distribution and apoptosis were measured by flow cytemetry. The protein expressions of ERK, JNK, and p38 were studied by Western blot. Results Dosage dependence was obviously showed after the different concentrations of Bullatacin were used to A549, and 25 μg/mL;Bullatacin blocked A549 cell in G0/G1 periods and induced its apoptosis. Compared with the blank group, protein expressions of P-ERK, P-JNK, and P-p38 were all increased by different degrees. Conclusion Bullatacin significantly inhibits the proliferation and induces the apoptosis of A549 cell. Its mechanism is related to activity of MAPK pathway thought the phosphorylation of the three protein kinases by Bullatacin.

Objective To discuss the value of emergency angiography and embolization treatment of hemorrhage of digestive tract.Methods Give emergency angiography and embolization treatment for 15 patients with hemorrhage of digestive tract.Male is 12.Famale is 3.Adopt to Seldinger technic and method of coaxial duct,the head of ducts accesses to the region of bleeding,with gelatin foam or suture to embolism.Results All 15 patients were sucessful embolismed and get objective of hemostasis.Angiography appeared contrast medium overflowing and the humens,mucosa development.All patients were not bleeding again.Conclusion Clinic efficiency of emergeney ;embolization treatment for hemorrhage of digestive tract is assurance and explanation the principle and events of attention.

Human resources are the key to TCM development. This article explored the effective channels to construct the system by deeply studying and identifying problem of the quantity, quality, configuration, development and training of TCM human resources under the characteristic medical reform of Gansu Province to provide references for better research and plan of TCM human resources for relevant department, further to realize the sustabile development of TCM characteristic medical reform in Gansu Province.

Objective: To evaluate the therapeutic effect of gamma knife on patients with intracranial cavernous angioma (CA). Methods: The medical records of 122 patients (134 lesions) who underwent radiosurgery were reviewed retrospectively. Results: hT e average follow-up period was 43 months. No patient died. One patient underwent CA resection. In patients with epilepsy, 83% patients showed alleviation of seizures. About 44% of the lesions shrank in size after treatment with gamma knife radiosurgery (59/134). Seven cases had hemorrhage again after radiosurgery, and the overall annual hemorrhage rate was 1.6%. Edema was found in 11.5% patients (14/122) and all patients showed improvement atf er treatment. Conclusion: Gamma knife is a safe treatment for CA, which could obviously improve the symptoms of epilepsy. Gamma knife radiosurgery is the if rst option for the treatment of cavernous sinus angiomas.

Objective To study the contrast enhanced magnetic resonance imaging (MRI)features of surgical margin and its path-ologic basis of the experimental rat model by resecting partial brain tissue.Methods Forty-two rats were randomly divided into six experimental groups (postoperative 1d,3d,7d,14d,21d,28d groups)and one contrast group.A partial resection of the parietooc-cipital region was performed.MRI procedures consisted of T1 WI and T2 WI and contrast enhanced T1 WI,pre-and postcontrast scan-ning were enforced in different postoperative days.Brain tissue samples were obtained after MRI examination.Pathological study was made and the results were compared with MRI findings.Results (1 )The earliest enhancement was observed around surgical margin on the first day after surgery,which enhancement was lasted until 28 days;(2)The enhancement of the surgical margin was significantly increased after 7d,the signal intensity ratio in this period reached the peak,but this ratio between 7d and 14d had no ob-vious disparation.The degree of enhancement gradually weakened in the next few days;(3)The number of neovessels reached the peak at 7d,which was higher than that at other experimental groups and contrast group (P <0.05);The amount of neovessels in 14d,21d and 28d had no obvious disparation (P >0.05).Conclusion The contrast enhanced MRI features of the surgical margins after resected partial brain tissue is related to the time of the postoperation,and the neovascularization plays an importent role in this process.

Objective To evaluate the long?term effect of the comprehensive control strategy for schistosomiasis with empha?sis on infectious source control in marshland?type endemic areas of Poyang Lake. Methods Three heavy endemic villages with schistosomiasis in the lake areas were selected as pilots for the comprehensive control strategy which included replacing bovine with tractors and forbidding grazing on marshlands. The data of schistosome infections of residents and Oncomelania hupensis snails were collected and analyzed statistically from 2009 to 2014. Results There were no patients with schistosomiasis through feces examinations in the 3 pilot villages. The average density of live snails in Niuzhou marshland showed a decline trend and the density was stable in Liulingwei marshland. There were no infected snails in the 2 marshlands and no schistosome adult worms were found in the sentinel rats Conclusion The implantation of the comprehensive control strategy for schistosomiasis with em?phasis on infectious source control can control the prevalence of schistosomiasis during a long period.

Objective To evaluate the effect of percutaneous transhepatic embolization of gastroesophageal varices on acute upper gastrointestinal bleeding in cirrhotic portal hypertensive patients. Methods Percutaneous transhepatic embolization was performed in 37 cases of acute upper gastrointestinal bleeding until varix thrombosed. Results Bleeding stopped in all patients. No technical complications occurred. During 1～38 months follow-up, variceal bleeding recurred in two cases in 3 months. One died of heart failure, five died of hepatic carcinoma in 6 months . Conclusion Percutaneous transhepatic embolization of gastroesophageal varices is a safe and effective treatment for acute upper gastrointestinal bleeding in portal hypertensive patients.

OBJECTIVETo compare the penetration abilities of resin infiltration into proximal lesions in primary molars and permanent posterior teeth in vitro.

METHODSExtracted or exfoliated primary molars (n = 32) and permanent posterior teeth (n = 36) with proximal white spot lesions were selected. Roots of the teeth were removed, and the crowns were cut across the caries lesions perpendicular to the surface. Cut surfaces were examined with stereo microscopy and classified with respect to histological lesion extension (C1-C4): Lesions confined to the outer half on enamel (C1), lesions confined to the inner half on enamel(C2), lesions confined to the outer half on dentin(C3), lesions extending into the inner half of dentin (C4). C4 were excluded. Caries lesions were etched for 2 min with 15% hydrochloric acid gel and were subsequently treated for 5 min with resin infiltration. Thin sections from each lesion were prepared and specimens were observed with confocal laser scanning microscopy (CLSM) through a dual-fluorescence staining technique. Lesion depth (LD) and penetration depth (PD) of resin infiltration were measured in primary molars and permanent posterior teeth in confocal microscopic images, and percentage penetration (PP) were calculated respectively. The date were collected and analyzed.

RESULTSThere were no significant differences for overall PP in primary molars and permanent posterior teeth groups (P > 0.05). However, the penetration abilities in primary molars were slight higher than those in permanent posterior teeth in C1 group (P < 0.05). The penetration abilities had no significant difference in primary molars and permanent posterior teeth in both C2 and C3 groups (P > 0.05).

CONCLUSIONResin infiltration have satisfied penetration abilities in primary molars and permanent posterior teeth with proximal initial lesions. The better penetration abilities of resin infiltration in primary molars are shown in Cl lesions than those in permanent posterior teeth.

OBJECTIVETo compare the penetration abilities of resin infiltration into natural initial caries lesions with those of adhesive in vitro.

METHODSThirty-six extracted human molars and premolars showing proximal white spot lesions were selected. Teeth roots were removed, and the crowns were cut across the caries lesions perpendicular to the surface. Corresponding lesion halves were etched for 2 min with 15% hydrochloric acid gel and were subsequently treated with either adhesive or resin infiltration. Specimens were observed with confocal laser scanning microscopy (CLSM) in dual fluorescence mode. In confocal microscopic images, penetration depth (PD) and lesion depth (LD) were defined as the distance from the surface to the deepest point of red and green fluorescence, respectively. The penetration percentages (PP) were calculated.

RESULTSAt the same level of caries, mean maximum lesion LD were comparable for both lesion halves (P > 0.05). But mean maximum PD and PP of the resin infiltration were significantly higher than those of the adhesive (P < 0.01).

CONCLUSIONPenetration of enamel caries lesions is observed in the adhesive and the resin infiltration. But the resin infiltration is capable of penetrating almost completely into enamel parts of natural caries lesions.

In 2023，World Health Organization（WHO）announced that the pandemic of COVID-19 is over；while there was an epidemic of multi-pathogenic infections in the respiratory tract by the end of the year in China，indicating that high attention still need to be paid to infectious diseases. In the past one year，some breakthroughs for infectious diseases have been achieved.This article reviews the major events and research progress in the diagnosis，prevention and treatment of infectious diseases in 2023.