ObjectiveTo analyze the expression and clinical significance of Maspin, vascular endothelial growth factor (VEGF) in endometrioid adenocarcinoma tissue samples. MethodsForty-nine cases of endometrioid adenocarcinoma, 12 cases of atypical hyperplasia of endometrium and 15 cases of normal endometrium were selected. Immunohistochemistry was used to study the expression of Maspin,as well as VEGF. ResultsThe expression of Maspin was none in normal endometrium,while the positive rates of Maspin in atypical hyperplasia of endometrium (41.7％, 5/12 ) and endometrioid adenocarcinoma (59.2％,29/49) were ascending, there was statistical significance among the three groups (P＜0.01 ). The positive rates of VEGF in normal endometrium ( 13.3％, 2/15 ), atypical hyperplasia of endometrium ( 33.3％, 4/12 ),endometrioid adenocarcinoma (67.3％ ,33/49) also were ascending (P＜ 0.01 ). The expression of Maspin was related with the FIGO stage in endometrioid adenocarcinoma (P=0.034), and the expression of VEGF was related with the myometrial invasion(P = 0.036). There was positve correlation between the expression of Maspin and VEGF (r = 0.307,P＜0.05). ConclusionsThe expression of Maspin, VEGF in tumor tissue has a significant correlation with the level of invasion of endometrioid adenocarcinoma. The expressions of Maspin,VEGF apparently play an essential role in the development and invasion of endometrioid adenocarcinoma.