1.Progress on relationship between gastric cancer and tumor microenvironment
Tao WANG ; Yongguang ZHENG ; Kangwu FAN ; Yumin LI
Practical Oncology Journal 2014;(6):561-564
Gastric cancer is the fourth malignancies and the second cancer -related mortality rate in the world.Although the incidence of gastric cancer has declined in the past few decades ,it is still a serious health problem.Tumor microenvironment plays an important role in tumorigenesis and progression .The main components of gastric cancer include tumor associated macrophages、lymphocytes、cancer-associated fibroblasts、angiogenesis factors,cytokines,gastric microenvironment and the mechanism of action of chemokines .In this review,the corre-lation between tumor microenvironment and GC is discussed .
2.Occluder effects on platelet function of patients with congenital heart disease undergoing transcatheter closure
Lin CHEN ; Hua YAN ; Liyuan CHEN ; Yongguang LU ; Chunhui FU ; Zhanchuan ZHENG
Chinese Journal of Tissue Engineering Research 2014;(16):2512-2518
BACKGROUND:Occluder implantation in patients with congenital heart disease can increase in vivo platelet adhesion and aggregation, resulting in thrombosis on the occluder surface. OBJECTIVE:To investigate the effect of the occluder on platelet function in patients with congenital heart disease undergoing transcatheter closure. METHODS: Clinical data from 124 patients with congenital heart disease undergoing transcatheter closure were retrospectively analyzed. These patients were divided into groups of atrial septal defect in 46 cases, patent ductus arteriosus in 43 cases and ventricular septal defect in 35 cases according to the types of congenital heart disease. The positive rates for peripheral blood CD62p, CD63 RESULTS AND CONCLUSION:There was no difference in the positive rates of peripheral blood CD and thrombin sensitive protein were compared before and 6 hours, 24 hours, 12 months after occluder implantation. 62p, CD63 and thrombin sensitive protein among three groups prior to occluder implantation. Up to 6 hours after occluder implantation, the expression levels of peripheral blood CD62p, CD63 and thrombin sensitive protein reached peak in the three groups, especialy in the patients with atrial septal defect and ventricular septal defect, then gradualy decreased. After 12 months, the expression levels of CD62p and CD63 recovered in the patients with patent ductus arteriosus and ventricular septal defect, but stil maintained a higher level in those with atrial septal defect (P < 0.05). The expression of thrombin sensitive protein showed no difference among the three groups at different time. These findings indicate that after occluder implantation, the platelet activation is more remarkable and lasts longer in the patients with atrial septal defect and ventricular septal defect, especialy in those with ventricular septal defect.
3.Current status and prospect of recuperative medicine
Yongguang ZHAO ; Weibing ZHANG ; Jun ZHENG ; Kuigao XU ; Qing WANG ; Huamiao SONG
Medical Journal of Chinese People's Liberation Army 2001;0(09):-
Objective To sum up the current status and developmental trend of researches on recuperative medicine worldwide,and to suggest the orientation,aims and focus of research work of recuperative medicine in PLA.Methods Review of published literature was used and on-the-spot investigation was performed to retrieve the publishments in concern and achievements in scientific researches acquired and published in recent years domestically and abroad,and analyze the new progresses and developmental trend of the subject of recuperative medicine.Results With the advances in modern science and technology,recuperative medicine has developed quickly in PLA in recent 5years,and outstanding achievements have been acquired in recuperative management,recuperative rehabilitation,recuperative nursing,recuperative psychology and recuperation for special troops.Conclusion Researches should be done in the future on some core techniques of recuperative medicine,such as natural factor therapy.Meanwhile,more attention should be paid on the recuperative prevention,recuperative health maintenance,recuperative treatment and recuperative rehabilitation for the servicemen who suffered physical and mental injuries induced by high nervous stress and compound risky factors under the condition of high-tech wars on carrying out risky and perilous tasks.
4.Diagnosis and treatment of giant hypertrophy of gastric mucosa
Peigui ZHANG ; Haisheng ZHOU ; Yongguang WANG ; Shaohua SHI ; Hanpeng ZHENG ; Xizhou LIN
Chinese Journal of Digestive Surgery 2021;20(3):352-354
Giant hypertrophy of gastric mucosa is rare and lack of typical clinical manifestations. The main treatment measures were minimally invasive surgery and drug intervention. Clinicians should pay attention to it's imaging features, in order to make early diagnosis and treatment, and obtain a good prognosis. The authors introduce the results of gastro-enterography and computed tomography in a case with giant hypertrophy of gastric mucosa, and differentiate the imaging results from gastric cancer, lymphoma and gastric stromal tumor, so as to provide references for the clinical diagnosis of the disease.
5.Gene clone, eukaryotic expression vector and tissue expression profile analysis of porcine BST-2 gene
Ning KONG ; Yongguang WU ; Qiong MENG ; Zhongze WANG ; Wu TONG ; Hao ZHENG ; Guoxin LI ; Tongling SHAN ; Enmin ZHOU ; Guangzhi TONG
Chinese Journal of Veterinary Science 2017;37(8):1594-1599,1640
In order to study the biological function of pig BST-2 gene,the BST-2 gene was amplified with specific primers from porcine kidney tissue,and molecular characterization of BST-2 nuclectide and amino acid sequence were analyzed with bioinformatics tools and online server.Then the prokaryotic expression and tissue expression profile analysis was carried out.The results showed that the full length of pig BST-2 gene was 851 bp and contained 23 bp of 5'-UTR,294 bp of 3'-UTR and 534 bp of CDS and the gene encoded 177 aa.Amino acid sequence analysis of pig BST-2 protein showed 46.1% identity with gorilla gorilla,41.7% with cricetulus griseus,39.5% with mus musculus,35.4% with equus asinus,42.0% with felis catus,40.5% with bos mutus,44.4% with macaca mulatta,38.7% with ovis aries and 46.8% with homo sapiens.BST-2 protein contained 2 transmembrane structure (27-49 aa and 154-176 aa),2 glycosylation sites and 14 potential phosphorylation sites including ATM,CK Ⅱ,PKA,PKC binding sites.The pig BST-2 protein was expressed in Vero cells after translated the recombinant plasmid FLAG-BST-2.Semiquantitative PCR results showed that BST-2 gene was expressed in all the tissues,especially in lymph nodes,thymus,tonsils,spleen,large intestine and small intestine.This study provide a foundation for further understanding the antiviral mechanism of pig BST-2 protein.
6.Application value of three-dimensional surgery planning system in the preoperative evaluation of primary liver cancer resection
Lili HUANG ; Pengfei ZHENG ; Jie MAO ; Fan LI ; Yongguang ZHENG ; Zhibin CHENG
Chinese Journal of Hepatic Surgery(Electronic Edition) 2014;(2):79-83
Objective To investigate the application value of three-dimensional surgery planning system in the preoperative evaluation of primary liver cancer (PLC) resection. Methods A total of 44 patients with PLC [32 males and 12 females, mean age of (60±12) years old] in Department of General Surgery, Lanzhou University Second Hospital from June 2012 to June 2013 were enrolled in this prospective study. The informed consents of all patients were obtained and the ethics committee approval was received. According to random number table method, the patients were randomly divided into 2 groups: computed tomography angiography (CTA) group and three-dimensional surgery planning (3D) group. Subgroups of complex PLC and non-complex PLC were further defined in each group according to tumor size, tumor invasive extent and history of surgery. In CTA group, 8 cases was assigned in complex PLC subgroup and 14 cases in non-complex PLC subgroup, and 6, 16 cases respectively in 3D group. CTA was used in the preoperative evaluation of PLC resection in CTA group. Liver three-dimensional surgery planning system was used in the preoperative evaluation of PLC resection in 3D group. The intra-operative finding was taken as a gold standard. The visualization of PLC, the adjacent relationship between PLC and peripheral tissues, the display rates of 12 abdominal vessels, variation of hepatic artery, vascular invasion of tumor, cholangiectasis by CTA and three-dimensional surgery planning system were observed. The relationship between estimated tumor volume by three-dimensional surgery planning system and actual weights of resected tumor was analyzed. Non-normal distribution data were expressed in M(Q25,Q75). The display rates by 2 methods were compared using Chi-square or Fisher's exact probability test. The relationship between estimated tumor volume by three-dimensional surgery planning system and actual weights of resected tumor was analyzed using Spearman rank correlation analysis. Results PLC lesions could be both visualized by 2 methods. The adjacent relationship between PLC and peripheral tissues could also be clearly visualized by three-dimensional surgery planning system. For patients with complex PLC, the display rate of abdominal vessels was 81%(78/96) in CTA group, and was 100%(72/72) in 3D group, where significant difference was observed (χ2=15.1, P<0.05). Cholangiectasis could not be visualized in CTA group and the display rate of cholangiectasis was 3/6 in 3D group. For patients with non-complex PLC, the display rate of abdominal vessels was 90%(151/168) in CTA group, and was 100%(192/192) in 3D group, where signiifcant difference was observed (χ2=20.39, P<0.05). Cholangiectasis could not be visualized in CTA group and the display rate of cholangiectasis was 19%(3/16) in 3D group. For patients with complex PLC, the median estimated tumor volume by three-dimensional surgery planning system was 218(129,429)ml and the actual weights of resected tumor was 194(112,429)g, where positive correlation was observed (r=0.943, P<0.05) with an average error rate of 6.5%. For patients with non-complex PLC, the estimated tumor volume by three-dimensional surgery planning system was 368(89,560)ml and the actual weights of resected tumor was 395(126,578)g, where positive correlation was observed (r =0.958, P<0.05) with an average error rate of 6.3%. Conclusions Compared with CTA, three-dimensional surgery planning system can better display the adjacent relationship between tumor and peripheral tissues, abdominal vessels, cholangiectasis and estimate the volume of resected tumor more accurately. It is especially suitable for patients with complex liver cancer.