Objective: To determine ? asarone and ? asarone in Rhizoma acori tatarinowii(RAT) . Methods: HPLC condition consists of ODS C 18 column(150mm?4.6mm, 5?m), methanol: water(6∶4) as mobile phase containing potassium dihydrogen phosphate 1.4g and sodium lauryl sulfate 1.2g per 1000mL, detective wavelength at 257nm, flow rate at 1.0mL?min -1 . Results: For RAT the mean recovery of 99.02%( RSD =1.03%) for ? asarone, 101.26%( RSD =3.57%) for ? asarone are obtained, respectively. Conclusion: The method is sensitive, rapid and accurate.