1.Preparation and cytotoxicity evaluation of cobalt doped nanotubular implant coating
Can CAO ; Lingzhou ZHAO ; Yanyan SONG ; Yonggang DANG ; Li ZHANG ; Yumei ZHANG
Journal of Practical Stomatology 2015;(2):175-179
Objective:To prepare a kind of titanium implant doped with cobalt and to study its cytotoxicity.Methods:The surface of the titanium was anodized to form TiO2 nanotube arrays.Different amount of cobalt was doped by hydrothermal treatment,which was controlled by tuning the hydrothermal treatment duration.The cytotoxicity of the cobalt doped nanotubular implant coating on bone marrow stromal cells (BMSCs)was measured by CCK-8.Results:The nanotubular implant coating with different amount of cobalt was fabricated.The proliferation of BMSCs was inhibited by the nanotubular morphology and cobalt doping.Samples formed by hydro-thermal treatment in 0.1 M cobalt acetate showed significantly cytotoxicity.Conclusion:Hydrothermal treatment of anodized titanium is an effective way for developing novel cobalt doped nanotubular implant coating.The proper dose of cobalt doping needs to be further investigated.
2.Globular adiponectin-mediated vascular remodeling by affecting the secretion of adventitial-derived tumor necrosis factor-α induced by urotensin II.
Jun LI ; Limin LUO ; Yonggang ZHANG ; Xiao DONG ; Shuyi DANG ; Xiaogang GUO ; Wenhui DING
Journal of Zhejiang University. Science. B 2022;23(12):1014-1027
OBJECTIVES:
In this study, we explored how adiponectin mediated urotensin II (UII)-induced tumor necrosis factor-α (TNF-α) and α-smooth muscle actin (α-SMA) expression and ensuing intracellular signaling pathways in adventitial fibroblasts (AFs).
METHODS:
Growth-arrested AFs and rat tunica adventitia of vessels were incubated with UII and inhibitors of signal transduction pathways for 1‒24 h. The cells were then harvested for TNF-α receptor (TNF-α-R) messenger RNA (mRNA) and TNF-α protein expression determination by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. Adiponectin and adiponectin receptor (adipoR) expression was measured by RT-PCR, quantitative real-time PCR (qPCR), immunohistochemical analysis, and cell counting kit-8 (CCK-8) cell proliferation experiments. We then quantified TNF-α and α-SMA mRNA and protein expression levels by qPCR and immunofluorescence (IF) staining. RNA interference (RNAi) was used to explore the function of the adipoR genes. To investigate the signaling pathway, we applied western blotting (WB) to examine phosphorylation of adenosine 5'-monophosphate (AMP)-activated protein kinase (AMPK). In vivo, an adiponectin (APN)-knockout (APN-KO) mouse model mimicking adventitial inflammation was generated to measure TNF-α and α-SMA expression by application of qPCR and IF, with the goal of gaining a comprehensive atlas of adiponectin in vascular remodeling.
RESULTS:
In both cells and tissues, UII promoted TNF-α protein and TNF-α-R secretion in a dose- and time-dependent manner via Rho/protein kinase C (PKC) pathway. We detected marked expression of adipoR1, T-cadherin, and calreticulin as well as a moderate presence of adipoR2 in AFs, while no adiponectin was observed. Globular adiponectin (gAd) fostered the growth of AFs, and acted in concert with UII to induce α-SMA and TNF-α through the adipoR1/T-cadherin/calreticulin/AMPK pathway. In AFs, gAd and UII synergistically induced AMPK phosphorylation. In the adventitial inflammation model, APN deficiency up-regulated the expression of α-SMA, UII receptor (UT), and UII while inhibiting TNF-α expression.
CONCLUSIONS
From the results of our study, we can speculate that UII induces TNF-α protein and TNF-α-R secretion in AFs and rat tunica adventitia of vessels via the Rho and PKC signal transduction pathways. Thus, it is plausible that adiponectin is a major player in adventitial progression and could serve as a novel therapeutic target for cardiovascular disease administration.
Mice
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Rats
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Animals
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Adventitia/metabolism*
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Tumor Necrosis Factor-alpha/metabolism*
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Calreticulin/metabolism*
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Vascular Remodeling
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AMP-Activated Protein Kinases/metabolism*
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Cells, Cultured
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RNA, Messenger/genetics*
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Inflammation