Objective To investigate the relationship between anti-trophoblast membrane antigens(TA) antibodies and hypertensive disorders complicating pregnancy(HDCP).Methods Enzyme linked immunosorbent assay(ELISA) was used to detect anti-TA IgG and IgM in both maternal and umbilical serum samples of 40 normal pregnant women and 92 HDCP women(23 gestational hypertension or mild preeclampsia,41 severe preeclampsia and 28 eclampsia).Results The positive rates of anti-TA IgG and IgM in maternal serum samples with HDCP,eclampsia or severe preeclampsia were significantly higher than that in normal pregnant women,the positive rate of anti-TA IgM increased significantly with the aggravation of HDCP(P

This study was designed to label rat bone marrow mesenchymal stem cells (rBMSCs) with humanized renillar green fluorescent protein (hrGFP) for exploring the detectable effects of hrGFP on the construction of tissue-engineered bone in vitro. The hrGFP expression plasmid was packed into lentivirus by 293FT cells and transduced into rat BMSCs. After transduction, 81.3% rBMSCs successfully expressed green fluorescence. The hrGFP-rBMSCs were statically loaded on Bioglass-Collagen-Hyaluronic Acid-Phosphatidylserine (BG-COL-HYA-PS) scaffold in complete L-DMEM medium or osteogenic medium for 14 days. At 6h, a number of cells expressing green fluorescence can be observed by fluorescent microscopy. At day 7 and 14 after co-culture, the number of cells on the scaffold gradually increased. After 14 days for osteogenic induction, the hrGFP-rBMSCs and the interior of scaffold can be detected the expression of type I collagen. The results demonstrate that hrGFP labelling technique can detect visualizedly and effectively cell adhesion, proliferation and osteogenic differentiation in the construction of tissue-engineered bone in vitro.
Animals ; Bone Marrow Cells ; cytology ; Cell Culture Techniques ; Cells, Cultured ; Green Fluorescent Proteins ; genetics ; metabolism ; Humans ; Mesenchymal Stromal Cells ; cytology ; metabolism ; Osteogenesis ; Rats ; Staining and Labeling ; methods ; Tissue Engineering ; methods ; Tissue Scaffolds