Objective To study the detection value of serum fibronectin,electrolyte and myocardial enzyme in neonatal hypoxic-ischemic encephalopathy (HIE).Methods Ninety children with HIE were selected as the observation group,among them 28 cases of mild,34 cases of moderate,28 cases of serious,and 45 cases of normal full-term infant were selected as control group.The serum fibronectin,electrolyte and myocardial enzyme of two groups were detected.Results The serum fibronectin level in observation group was significantly lower than that in control group [(141.23 ± 45.21) g/L vs.(257.51 ± 28.99) g/L,P＜ 0.05].With clinical degree increased,the serum potassium level in observation group gradually increased,but the difference between control group and observation group,and among different clinical degree of observation group were not statistically significant (P ＞ 0.05).The serum sodium level gradually reduced,there were significant differences among mild,moderate,serious [(130.34 ± 3.22),(126.67 ± 3.54) and (122.45 ±2.80) mmol/L,respeetively,P ＜ 0.05],they were significantly lower than control group [(136.22 ± 6.21)mmol/L,P ＜ 0.05].The serum chlorine and calcium levels gradually reduced,mild were (98.99 ± 2.76) and (0.96 ±0.26) mmol/L,moderate were (97.21 ±3.41) and (0.95 ±0.28) mmol/L,and serious were (90.33 ± 3.09) and (0.71 ± 0.18) mmol/L,there was no significant difference between moderate and mild (P ＞ 0.05),serious were significantly lower than those in mild and moderate (P ＜ 0.05).The serum chlorine and calcium levels in mild,moderate and serious were significantly lower than those in control group [(103.43 ±3.21) and (1.42 ±0.01) mmol/L,P ＜0.05].With clinical degree increased,the aspartate aminotransferase,lactate dehydrogenase,creatine kinase,creatine kinase isozyme MB and hydroxyl butyrate dehydrogenase levels in observation group gradually increased,mild were (46.2 ± 12.3),(432.1 ± 145.2),(245.3 ± 104.2),(40.3 ± 7.9) and (374.1 ± 148.2) U/L,moderate were (84.4 ± 33.1),(569.3 ± 168.2),(845.1 ± 498.2),(112.1 ± 50.4) and (473.8 ± 139.2) U/L,serious were (122.1 ± 45.6),(966.9 ± 407.2),(1851.3 ± 912.3),(175.1 ±-55.6) and (834.1 ± 420.4) U/L,they were significantly higher than those in control group [(35.1 ±7.6),(261.9 ±47.1),(117.8 ±39.7),(11.4± 5.2) and (203.1 ±42.1) U/L](P＜0.05),and among different clinical degree of observation group were statistical significant (P ＜0.05).Conclusion The detection of serum fibronectin,electrolyte and myocardial enzyme in HIE children has important clinical practice significance,and can be used to evaluate the illness and prognosis.

ObjectiveTo investigate the effect and safety of salmeterol combined with fluticasone propionate dry powder in the treatment of cough variant asthma(CVA).Methods45 children diagnosed as CVA(aged from 5 to 14 years old)were randomly divided into treatment group(n =23)and control group(n =22).The patients in treatment group were treated with salmeterol combined with fluticasone propionate every morning and evening,while the control group were only given fluticasone propionate every morning and evening.The two groups were treated for 3 months.The change of clinical symptoms,asthma control time,lung function (PEF％ pred) and adverse reactions were observed before and after treatment.ResultsCough was relieved in most cases after treatment in both groups,but the improvement was more significant in treatment group than that in control group 1 week after treatment( x2 =10.24,P ＜ 0.05).However,there were no significant differences of clinical efficacy between two groups 2 weeks later( P ＞0.05).Full control time of asthma was in treatment group,which was significantly earlier than the control group( t =8.36,P ＜ 0.05 ).PEF％ pred was shorter significantly higher in two groups after 1 week,2 and 4 weeks,but the improvement was more significant in treatment group than that in control group( t =6.22,7.68,7.95,all P ＜ 0.05 ).1 case of headache in treatment group was relieved by symptomatic treatment and no other serious adverse events occured.ConclusionSalmeterol combined with fluticasone propionate was safe,effective,easy to use for treating CVA.

Using an anti-nucleolar protein B23 monoclonal antiboby, we examined the changes of protein B23 content in NIH3T3 fibroblasts during serum-induced proliferation and the difference in protein B23 content between mitotic and interphase NIH3T3 fibroblasts by immunofluorescence and im-munoblot methods. When NIH3T3 cells grew confluent, the serum containing medium was removed and replaced by serum-free medium,and the cells were cultured for another 24h. Then cells were refed with serum and further cultured. The results showed that protein B23 content was low in serum-starved NIH3T3 cells,and it markedly increased after serum addition. Furthermore,the increase occurred early at 6h after serum treatment. It was also showed that protein B23 content in mitotic cells blocked by the microtubule inhibitor colcemid was much higher than that in interphase ones. Our results suggested that nucleolar protein B23 might play some roles in the early stage of serum- induced proliferation and during transition from G2 to mitosis in cell cycle.

BACKGROUND:Silicon carbide-carbon (C/C-SiC) composite materials are widely used, but no relevant experimental studies on medical prosthesis materials have been reported.
OBJECTIVE: To demonstrate the feasibility of C/C-SiC composite materials as a substitute for facet joint prosthesis based on the biomechanical research and three-dimensional finite element analysis.
METHODS:The predetermined size C/C-SiC composites and traditional carbon/carbon(C/C) composite materials were placed in an electronic universal testing machine, to measure and calculate material compressive elastic modulus, compressive strength, maximal anticompression force, flexural modulus, bending strength and maximal antibending force. Afterwards friction coefficient, wear volume and weight wear rate were measured. Using three-dimensional finite element analysis, finite element models of the third metacarpal bone were defined as C/C and C/C-SiC composite element types, respectively. 200 N axial force was applied to analyze the total displacement and node stress.
RESULTS AND CONCLUSION:Compressive elastic modulus, compressive strength,maximal anticompression force, flexural modulus, bending strength and maximal antibending force of C/C-SiC composites were significantly higher than those of the C/C materials (P < 0.05); friction coefficient, wear volume, weight wear rate, total displacement as wel as node stress of C/C-SiC composites were significantly lower than those of C/C materials (P < 0.05). These results prove that C/C-SiC composite has favorable mechanical properties, antiwear ability, compression resistance and stress resistance.

Objective To explore the mechanism of Exenatide-induced rat pancreatic tissue lesion.Methods Thirty SD male rats were divided into three groups according to complete random design,and each group had 10 rats,namely Exenatide group,diabetes-model group and control group.Diabetes-model rats were induced by streptozotocin (STZ,35mg/kg) and high-sugar and high-fat diet.The Exenatide group and diabetes group were subcutaneously administered with Exenatide at a dose of 5 μg/kg twice a day.The control group was treated with same amount of saline.Ten weeks later,all the rats were sacrificed and the pancreatic tissues were harvested for routine pathological examination.Immunohistochemical method was used to detect the expression of α-smooth muscle actin (α-SMA) and type Ⅲ collagen protein in pancreatic tissue,and ELISA was applied to measure the expression of matrix metalloprotei-nase-2 (MMP-2) and MMP-9 in pancreatic tissue.Results In control group,there was no pathological change in pancreatic tissue.In Exenatide group,chronic inflammatory changes were observed; and the degree of inflammatory changes were much severe in diabetes group,and the pathological scores were gradually increased in the 3 groups (P ＜0.05).The expressions of MMP 2 in pancreatic tissue in control group,Exenatide group,diabetes group were (186.98 ± 23.24),(306.07 ± 59.82),(365.08 ± 89.55) μg/L,and the expressions of MMP-9 were (49.37 ± 7.08),(67.24 ±14.73),(87.37 ±13.39)μg/L.The values were significantly higher in Exenatide group and diabetes group than those in control group (P ＜ 0.05),but the difference between the two groups was not statistically significant.The numbers of α-SMA positive cells per high power field were (13.4 ± 5.97),(29.5 ± 8.80),(79.3 ± 27.23) in control group,Exenatide group,diabetes group,and the numbers of type Ⅲ collagen positive cells were (10.6 ± 4.93),(29.3 ± 12.95),(56.0 ± 27.21).The values were significantly higher in Exenatide group than those in control group,and the values were significantly higher in diabetes group than those in Exenatide group (P ＜ 0.05).Conclusions Long-term subcutaneous injection of Exenatide may activate pancreatic stellate cells and cause expression of α-SMA,Ⅲ collagen protein,and MMP-2,MMP-9,then induce chronic inflammatory changes.

10.3969/j.issn.1000-3606.2013.06.009

[Objective] In order to explore the optimal minor-factor culture system of CIK/NK cells by proliferating CIK/NK cells using single-factor,bi-factor,and tri-factor combinations of IL-2,IL-7,and IL-12.[Methods] Ficoll-Hypaque method was used to separate cord blood mononuclear cells and divide them into 6 groups.Add single-factor,bi-factor,and tri-factor combinations (IL-7;IL-12;IL-7 + IL-12;IL-2 + IL-7;IL-2 + IL-12;IL-2 + IL-7 + IL-12) of IL-2 (80 ng/mL),IL-7 (40 ng/mL),and IL-12 (40 ng/mL) and culture them in total 21 days then harvest the cells.To collect cell suspensions of each group in culture outset and day 21 and to count the proportion of CD3+ CD56+ CIK cells and CD3- CD56+ NK cells with flow cytometry.[Results] The proportion of CIK cells in single-factor culture system of IL-12 was the highest in all groups (30.23 ± 1.18%).The proportion of CIK cells in bi-factor culture system of IL-2 + IL-12 can raised to 18.58 ± 0.68%.The proportion of CIK cells of the two groups above can reach the level of that using traditional multi-factor culture methods.NK cell proportion in IL-12 was 30.23 ± 1.18%,NK cell proportion in IL-2 + IL-7 can also reach to 29.52 ± 0.89%.[Conclusions] It is adoptable to proliferate CIK/NK cells using minor-factor culture system of IL-12,IL-2 + IL-12,or IL-2 + IL-7.

Objective To investigate the molecular epidemiology and risk factors of the expression of the efflux pumps of multidrug resistant Pseudomonas aeruginosa (Pa). Method From April 2014 to October 2014 , clinical features of 100 patients infected with multidrug resistant Pa were retrospectivly analyzed . Results MexAB-OprM was expressed in all multidrug resistant Pa strains (100%). The expression rates of MexEF-OprN and MexCD-OprJin the multidrug resistant Pa strains were 50% and 36%, respectively. The risk factor of the three efflux pumps expression was analyzed and the results were as follows: the use of carbapenems and glycopeptides antibiotics was the risk factor of MexAB-OprM overexpression which was induced. The use of macrolide and carbapenems antibiotics was the risk factor of the induced MexEF-OprN and MexCD-OprJ expression. Invasive procedures, low WBC, anaemia, chronic fundamental disease and hospitalized in ICU ward were the common risk factors of communicable expression or overexpression of the three efflux pumps. Invasive procedures, low WBC, anaemia, chronic fundamental disease and hospitalized in ICU ward were commonly independent risk factors of expression of the three efflux pumps. Conclusion The normalized use of carbapenems and glycopeptides antibiotics , the reduction of unnecessary invasive procedures , the severely grasped standard of hospitalized in ICU ward , curing the fundamental diseases and improving the immunonity played important roles in the prevention of the expression or overexpression of the efflux pumps of multidrug resistant Pa.

Objective To observe the clinical efficacy of plasma exchange(PE),PE combined with plasma bilirubin absorp-tion(PE+PBA),and PE combined with double plasma molecule absorption system(PE+DPMAS),to investigate the best treat-ment options for the patients of hepatitis B-associated subacute severe.Methods Totally 140 patients who had hepatitis B-associat-ed were randomly divided into a PE group,a PE+PBA group,and a PE+DPMAS group.The dinical symptoms and blood rontine, electrolytc and the main biochemical indexes were recorded both before and after treatment and compared among the three groups. Results The total effective rate was higher in the PE +DPMAS group than in the PE group and PE+PBA group,which were 70.8%,60.9%,67.4% respectively,but there was no significant difference(P >0.05).compared with before treatment,serum total bilirubin (TBIL),alanine aminotransferase (ALT),aspartic acid amino shift enzyme (AST),valley aminoacyl transfer peptidase (GGT),alkaline phosphatase (ALP),globulin (GLB)and other biochemical indexes decreased significantly after treatment in 3 groups (P <0.001).PA was increased in the PE group and decreased in the PE+PBA and PE+DPMAS group after treatment,the differences were significant(P <0.05).After treatment,PT was shortened in the PE group,the differences were significant(P <0.05),but there were no significant difference between the PE+PBA and PE+DPMAS groups(P >0.05).Serum K+ and Cl- was obviously declined after treatment in the PE group,there was significant difference(P <0.001).Serum Na+ was obviously declined after treatment between the PE + PBA and PE + DPMAS groups (P < 0.001 ),but the difference was no significant in the PE groups.Serum Ca2+ was significantly decreased in the three groups of patients after treatment(P <0.001).WBC,Hb and PLT were significantly statistical difference after treatment(P <0.05).Conclusion The three groups also can improve liver function and the treatment rate for the patients of subacute hepatitis B-associated severe hepatitis.PE+PBA and PE+DPMAS groups can effective-ly reduce two thirds of the overall usage of the plasma.PE+DPMAS groups that the quantity of Cl- ,Ca2+ ,Hb decline much least in the three groups show larger value in the clinical application.