OBJECTIVE To investigate multidrug-resistant bacteria infection in newborn department,for rationally use of antibiotic. METHODS A total of 1927 patients in newborn department scientific were investigated for their multidrug-resistant bacteria infection. RESULTS There were no statistical differences of multidrug resistance bacteria infection in the four seasons. From 1927 newborn patients,128 patients were with multidrug-resistant bacteria infection,the positive rate was 6.64%,83.59% origin of Society area,in 128 patients children of multidrug resistance bacteria infection,happen infection were 134 example 86.57% were respiratory tract infection,70.83% resistance bacteria including Klebsiella pulmonary,appear multidrug resistance bacteria infection previous 62.28% were use antibiotic. CONCLUSIONS Newborn deartment The multidrug resistance bacteria infection is related to occurved in newborn department abuse and unrationaly use of antibiotics. Its should be strengthened to supervise and manage.

0.05]. CONCLUSIONS: The currents of voltage-gated potassium channel was inhibited by chronic hypoxic. The inhibitory effect of cGMP on currents of voltage-gated potassium channel in PASMCs from both normal and chronic hypoxic rats may be probably through the phosphorylation of voltage-gated potassium channel.

The function of salivary glands in patients with subacute thyroiditis was evaluated.The data of patients with subacute thyroiditis,primary hypothyroidism,and normal controls undergoing radionuclide scanning were reviewed and compared retrospectively.The ratio of target to non-target values in the salivary glands were decreased in patients with subacute thyroiditis.Thyroid and salivary glands in patients with subacute thyroiditis might be damaged simultaneously and both developed pharyngeal symptoms partly due to the damaged salivary glands.

Objective: To investigate the effect of Helicobacter pylori (H. pylori)　on the synthesis of cyclooxygenase-1 (COX-1)　and cyclooxygenase-2 (COX-2) in gastric epithelial cells. Methods: a VacA(+) and CagA(+) international standard H. pylori line NCTC11637 and a human gastric epithelial carcinoma cell line BGC-823 were used. Western Blotting was applied to detect the synthesis of cyclooxygenase. Results: The content of COX-2 protein increased obviously after the cells were incubated with H. pylori sonicating extract for 1　h and the increase lasted for at least 6　h whereas the content of COX-1 protein did not change during the incubation with H. pylori extract. H. pylori lipopolysaccharide (LPS) had no effect on COX-2 synthesis. Conclusion: H. pylori stimulated the synthesis of COX-2 in BGC-823 cells and the effect was LPS-independent.

Objective To analyze the clinical efficacy of endovenous laser treatment combined with transilluminated powered phlebectomy for great saphenous varicosity and summarize the experiencein order to achieve better results.Methods From January 2014 to June 2015,45 patients (52 limbs,including 17 males and 28 females,aged 36 to 68 years) diagnosed as great saphenous varicosity received endovenous laser treatment combined with transilluminated powered phlebectomy.Thirty-eight cases was unilateral great saphenous vafcose veins,7 cases was bilateral large saphenous varicose veins.The clinical efficacy was analyzed.Results All cases were performed successfully.The mean operation time was (68.4 ± 15.1) minutes,the average bleeding volume was (23.5 ± 10.7) ml,the number of mean operative incision quantity was (5.8 ± 1.9) and the average hospitalization time was (5.9 ± 1.6) days.All patients had no serious complications.During a mean follow-up time of 22 months (12 to 30 months),only one patient recurred.Conclusions Endovenous laser treatment combined with transilluminated powered phlebectomy for great saphenous varicosity is effective and safe.It also has the advantages of shorter operation time,less bleeding,shorter hospitalization time,small trauma and better cosmetic results.

In order to meet the demand of higher medical education reform and to enhance the re-search capability among medical undergraduates , Capital Medical University carried out a project named undergraduate scientific research and innovation program. In the process of the project , several problems were found in both undergraduates and tutors including lack of document indexing skill , professional English ability , doctor-patient communication consciousness , data statistics and paper writing skills of undergraduates and limited time, insufficient funds and lacking experiences of tutors. Through establish-ing sound and standard training system, increasing scientific research funding, setting up incentive mech-anism and increasing scientific research ability training courses, scientific research ability of undergradu-ates and tutors were firmly strengthened.

OBJECTIVE To explore the isolation,distributive characteristics and drug resistance of Acinetobacter baumannii producing AmpC enzyme in nosocomial infection.METHODS The distributive sections and infected parts of 42 A.baumannii strains in nosocomial infection were analyzed,and three dimensional test was used to(detect) AmpC enzyme.RESULTS Among them 18(42.86%) strains were AmpC positive.Among 40 strains of A.baumannii in non-nosocomial infection,6(15.00%) strains were AmpC positive.A.baumannii in nosocomial(infection) in clinical sections was mainly discovered in burn department,and the respiratory tract and skin soft tissue were the main infected sites(90%).The drug resistance in nosocomial infection was obviously higher than that of A.baumannii in non-nosocomial one.CONCLUSIONS The isolated rate and drug resistance rate of A.baumannii producing AmpC enzyme are rather high in nosocomial infection.It′s(neccessary) to take measures to prevent the nosocomial infection caused by A.baumannii.

Objective To investigate the influence of application of local anesthesia with lidocaine on bronchial lavage fluid (BLF) pseudomonas aeruginosa culture and drug sensitivity in cases with lung infection .Methods Two hundred and seventy speci-men of BLF were collected from 135 patients with infection of lung .And BLF were collected directly from right-broncho in control group ,and from left-broncho in lidocaine group .The outcome of pseudomonas aeruginosa culture and drug sensitivity were com-pared in the two groups .Results Fourty-two cases were postitive in BLF pseudomonas aeruginosa culture in the control group ,and 40 cases were postitive in lidocaine group .The positive rates were 31 .11% and 29 .63% ,respectively .There were no significance between the two groups (P<0 .001) .Compared with the control group ,the sensitive strains of pseudomonas aeruginosa were obvi-ously less and the drug tolerance strains were much more in lidocaine group for Ciprofloxacin and Levofloxacin (P<0 .05) .Howev-er ,there were no influence for drugs such as Piperacillin/Tazobactam and Ceftazidime ,etc .Conclusion 2% lidocaine has no influ-ence on the outcome of BLF pseudomonas aeruginosa culture .But it may reduce the drug sensitivity of Ciprofloxacin and Levofloxa-cin in cases with infection of lung .

Objective To investigate the infection status of hepatitis B virus(HBV) and hepatitis C virus(HCV) in healthy adult in Xi′an ,in order to provide references for hepatitis prevention and health education .Methods A total of 1 052 healthy adults from 10 communities in Xi′an were collected by using multistage stratified random sampling method .HBV serum markers and anti‐HCV antibody were detected ,and characteristics of distribution of HBV and HCV infection were analysed .Results A total of 37 adults were observed with positive hepatitis B surface antigen(HBsAg) ,the total positive rate was 3 .52% ,and the positive rate of male and female was 4 .08% and 3 .07% respectively ,no statistically significant difference was found between male and female(P>0 .05) .Apart from male adults aged 30 - < 40 and 40 - < 50 ,there were no statistically significant differences between each age groups in male and female ,and between female and male in the same age groups(P> 0 .05) .A total of 31 adults were observed with HBsAg ,hepatitis B e antibody (HBeAg)and hepatitis B core antibody(HBcAb) positive ,the positive rate was 2 .95% .The positive rate of hepatitis B surface antibody(HBsAb) was 54 .18% .Serological patterns between different gender had no statistically signifi‐cant differences(P>0 .05) .A total of 9 adults(0 .86% ) were observed with positive anti‐HCV antibody ,and the positive rate of male and female was 0 .64% and 1 .02% respectively ,no statistically significant difference was found between male and female(P>0 .05) .Conclusion Healthy adults in Xi′an have relatively low infection rates of HBV and HCV ,while for the control of hepatitis B and hepatitis C ,further strengthening the health education ,improving awareness of prevention and routine monitoring infectious dis‐eases and vaccination may still be necessary .

Objective To investigate the relationship between suspected ankylosing spondylitis and HLA‐B27 antigen by detec‐ting the positive frequency of HLA‐B27antigen in 872 suspected AS patients ,and evaluate its clinical significance .Methods The positive frequency of HLA‐B27 on the T lymphocyte membrane were detected by flow eytometer in 872 suspected AS patients .Re‐sults Among the 872 suspected AS patients the ratio between male and female was 1 .8∶1 ,the positive rate of antigen HLA‐B27 was 27 .29% ,and the male and female patients′positive rates of HLA‐B27 antigen were 32 .50% and 17 .95% ,respectively (P<0 .05) .The male and female patients′ expression percentage of B27+ /B7- monoclonal antibody were 39 .16 ± 42 .79 and 20 .96 ± 33 .86 ,respectively(P<0 .05) .The male and female patients′mean fluorescence intension of B27+ /B7- monoclonal antibody were 5 .35 ± 5 .44 and 3 .35 ± 3 .87 ,respectively(P<0 .05) .Conclusion The patients with AS are strongly associated with HLA‐B27 an‐tigen .Detection of HLA‐B27 antigen expression intensity in suspected AS patients with FCM is helpful to diagnosis and differential diagnosis of AS .