The first version of The Clinical Guideline for Smoking Cessation in China was issued in 2007,which indicated that smoking cessation was considered to be a specialty and an academic field for physicians.Firstly,the Guideline described the epidemiology of cigarette smoking in China,a prevalence of 35.8% in a population 15 years and older by a nation-wide survey in 2002.The Guideline emphasized that tobacco dependence was a chronic and highly recurrent disease,which needed long-term treatment.Detailed intervention measures were provided for clinical practice.

Objective To evaluate the value of thoracoscopy in the diagnosis ot pleural effussion. Methods 45 cases of pleural effussion were exeamined by thoracoscopy, biopsy took place in the abnormal pleurae under thoracoscopy. Results Positive rate was 91.1% (41/45) under thoracoscopy.The morphology of pleural mesothelioma, metastatic carcinoma and tuberculous pleurisy was different. No serious complication occurred in all cases. Conclusions Thoracoscopy is a safe and effective procedure in the diagnosis of pleural effussion.

In order to meet the demand of higher medical education reform and to enhance the re-search capability among medical undergraduates , Capital Medical University carried out a project named undergraduate scientific research and innovation program. In the process of the project , several problems were found in both undergraduates and tutors including lack of document indexing skill , professional English ability , doctor-patient communication consciousness , data statistics and paper writing skills of undergraduates and limited time, insufficient funds and lacking experiences of tutors. Through establish-ing sound and standard training system, increasing scientific research funding, setting up incentive mech-anism and increasing scientific research ability training courses, scientific research ability of undergradu-ates and tutors were firmly strengthened.

OBJECTIVE:To provide reference for improving the education of rational drug use and skills for clinical medical students in the internship period. METHODS:Teachers in the respiratory department of our hospital took part in training interns and clinical pharmacists took part in teaching rounds to train about medication education and skills,including linking up the theoret-ical knowledge of rational drug use,training of skills about issuing rational prescriptions and judging drug information,guiding to participate in the education of rational use of drugs to patients and evaluating the knowledge and skill of rational drug use. RE-SULTS & CONCLUSIONS:Carrying out rational drug use education in practice will improve the mastery of knowledge of rational drug use,enhance the awareness of rational drug use and the idea,which is helpful for achieving the irrational drug use in clinical practice,and improve the overall quality of medical services. but there still remain some problems to be explored.

Objective To investigate nutrition status and dyspnea in the patients with chronic obstructive pulmonary disease (COPD) between GOLD Ⅱ and GoLD Ⅲ, and test the evaluative validity of disease status by GOLD classification of COPD. Methods Thirty patients with clinically stable COPD were recruited, including 15 patients of GOLD Ⅱ of COPD and 15 patients of GOLD Ⅲ of COPD. Body mass index (BMI), triceps skin-fold thickness (TSF), serum albumin (Alb), and partial pressure of oxygen in arterial blood (PaO2) were measured in each patient. Dyspnea was assessed by the Borg Scale (BS). Exercise stress test was taken by incremental exercise test. Results BMI was significantly lower in the patients of GOLD Ⅲ than that in the patients of GOLD Ⅱ[(19±5 ) kg/m2 vs (23±3) kg/m2,p < 0.05]. TSF was significantly reduced in the patients of GOLD Ⅲ than that in the patients of GOLD Ⅱ[ (8±3) mm vs(13±5) mm, P < 0.01]. Alia in the patients of GOLD Ⅲ was significantly decreased than that in the patients of GOLD Ⅱ [(32±7) g/L vs (36±6) g/L, P <0.05]. The difference of PaO2 between the patients of GOLD Ⅲ and the patients of GOLD Ⅱ was significant [(72±9) nun Hg (1 mm Hg = 0.133 kPa ) vs (78±8) nun Hg, P < 0.01], and the significant difference of BS was found between the patients of GOLD Ⅲ and the patients of GOLD Ⅱ( 5.0±2.0) grades vs (3.0±1.0) grades, P <0.05 ]. In addition, 12 patients in the patients of GOLD Ⅱ took the exercise stress test and 8 patients were found anaerobic threshold (AT), 5 patients in the patients of GOLD Ⅲ took the exercise stress teat and no AT was found. Conclusions The parameters of BMI, TSF, Alb and PaO2 are significantly reduced in the patients of GOLD Ⅲ than those in the patients of GOLD Ⅱ. In the patients of GOLD Ⅲ, BS is higher than that in the patients of GOLD Ⅱ and AT is difficult to obtain, suggesting more severe in degree of impairment. GOLD classification of COPD reflects the disease stares and prognosis in the patients with COPD, as a valuable parameter in clinical practice.

Objective To investigate the role of interleukin-4 (IL-4) and interleukin-5 (IL-5) in the pathogenesis of steroid-resistant (SR) asthma.Methods Fifteen patients with SR asthma and 15 patients with steroid-sensitive (SS) asthma were selected based on their clinical responses to prednisone therapy. The peripheral blood mononuclear cells (PBMCs) were obtained and incubated with phytohemagglutinin (PHA) in vitro for 48 h in the presence or absence of dexamethasone (10-7 mol/L). Expressions of IL-4 and IL-5 mRNA in PBMCs were determined by reverse transcription-polymerase chain reaction (RT-PCR), and mRNA positive PBMCs for IL-4 and IL-5 were measured with in situ hybridization using digoxin-labeled cDNA probes.Results In the absence of dexamethasone, there was no significant difference in the expressions of IL-4 and IL-5 mRNA between SR and SS asthmatics, and the numbers of mRNA positive cells for IL-4 and IL-5 were also similar between these two groups, however, in the presence of dexamethasone (10-7 mol/L), expressions of IL-4 mRNA (P<0.01) and IL-5 mRNA (P<0.05) were significantly inhibited in SS asthmatics, but not in SR asthmatics (P>0.05). The numbers of IL-4 and IL-5 mRNA positive cells decreased from 5.50±1.60 to 2.27±0.98 (P<0.01) and from 5.03±1.29 to 1.67±0.70 (P<0.01), respectively in SS asthmatics, but in the patients with SR asthma, there was no significant change in the number of cells expressing mRNA for IL-4 or IL-5.Conclusion The gene expression of IL-4 and IL-5 in SR asthma was not inhibited by dexamethasone at the concentration of 10-7 mol/L. It is suggested that the relative unresponsiveness of T cells to glucocorticoids and persistent production of cytokines may be one of the mechanisms of steroid resistance in asthma.

Purpose:To investigate the clinical effects of pneumatic lithotripsy (PL) under a ureteroscope for the treatment of ureteral stones.Method:From April 1996 to April 2000,252 patients with ureteral stones undergoing PL was retrospectively analyzed.Results:Among 252 cases, 238 had a satisfactory therapeutic effects with the successful rate being 94.4 %.8 cases failed to placement of ureteroscope and the successful rate for the insertion of ureteroscope was 96.8 % (244/252). During operation, ureteral stones in 6 cases were not dealt with well with the successful rate of PL being 97.1% (238/244).Conclusion:The therapeutic effects of PL under ureteroscope in the treatment of ureteral stones were reliable and the recovery after operation was rapid.PL was an ideal therapy for ureteral stones.

OBJECTIVETo study the cause of the seeds dormancy of Glehnia littoralis in vitro and to establish plant regeneration methods via somatic embryos.

METHODThe effects of endosperm and exogenous hormone on the seed dormancy breaking of G. littoralis and the effect of hormone concentration on embryonic callus induction and plant regeneration via somatic embryos were observed,

RESULTSThe germination rate of the seeds with 1/3 endosperm was the highest which achieved 31%. TDZ, 6-BA and GA3 treatment could not break seed dormancy but easily lead to abnormal seedlings. Embryogenic callus induction rates was up to 57% on MS supplemented with 1.0 mg x L(-1) 2,4-D. After 20 days culture, embryogenic calli were transferred to MS medium and cotyledonary embryos were formed in 40 days. The regenerated plants were obtained in 20 days.

CONCLUSIONAn effective system of plant regeneration of G. littoralis was established in this study.

Objective To investigate the effects of the fibrin scaffold on the differentiation and the proliferation of neural stem cells and astrocytes. Methods Neural stem cells and the gliocytes derived from spinal cord were cultured in vitro respectively. The purified neural stem cells or gliocytes were seeded separately onto the fibrin scaffolds as experimental group and the glass slides modified with poly-L-lysine(PLL)as control group. At different time in culture the neural stem cells were immunofluorescence stained with antibodies against the marker of neurons I.e. Neurofilament(NF).The length of NF-positive neuritis was masured and the average value was calculated in the culture well (n=4). The gliocytes were immunofluorescence stained with antibodies against the marker of astrocytes I.e. Glial fibrillary acidic protein (GFAP ). The total number of the cells and the GFAP-positive cells were counted from 5 different fields of vision in the culture well (n=4), then the average ratio of GFAP-positive cells was calculated. The differentiation of neural stem cells, the extension of neurites and the proliferation of astrocytes on the fibrin scaffolds were compared with those on the slides. The protein of GFAP was detected by Western blotting to analyse the mature degree of astrocytes. All above experiments were repeated 3 times respectively. Results Immunofluorescence staining showed that the NF-positive neurites in the fibrin scaffold group were longer than those in the control group, whereas GFAP-positive cells were fewer than those in the control group. The expression of GFAP in the cells on the scaffold was lower than that in the control group.Conclusion The fibrin scaffold could promote differentiation of the neural stem cells to neurons and extension of the neurites. Meanwhile, the scaffold could inhibit proliferation and mature of the astrocytes.

Objective To analyse pathogenesis, types, diagnosis and operation methods of adult intussusception. Methods Clinical data of 150 patients with adult intussusception were reviewed retrospectively . Results Symptom included paroxysmal bellyache (90 0%), abdominal mass (64 7%), nausea and vomit (58 0%), hematochezia (20 7%), constipation (10%) and symptom triad of bellyache , mass and hematochezia (15 3%). Tumor, inflammation, Meckel′s diverticulum and mobile cecum were main cause for intussusception. All 150 patients received operation, 147 patients were cured and 3 patients died. Conclusions Most patients of adult intussusception had pathological basis. The result of surgical therapy is good.