Objective To explore the feasibility for determination of chloroform and tetrachloromethane in drinking water with DB-5, Rtx-1 and DB-1 capillary columns. Methods For the mild solubility of chloroform and tetrachloromethane in drinking water, chloroform and tetrachloromethane were analysed by static headspace gas chromatography with DB-5, Rtx-1 and DB-1 capillary columns and electron capture detectorECD, external standard method was used for quantification. Results Three kinds of columns were used for determination of chloroform and tetrachloromethane,the retention time was lower than 3 min, for chloroform and tetrachloromethane the average recovery rates were 92.06%-104.95% and 78.33%-103.22% respectively, RSD were 2.06%-2.71% and 1.76%-5.59% respectively. Conclusion DB-5, Rtx-1 and DB-1 capillary columns are suitable for determination of chloroform and tetrachloromethane in drinking water.

Objective To investigate the organochlorine and pyrethroid pesticide residue in leafy vegetables on sale in Xi’an and to assess the safety of the leafy vegetables. Methods Eight kinds of leafy vegetables samples were collected at five wholesale markets and five supermarkets in Xi’an from 2007 to 2008. The content of 15 kinds of pesticides residues were analyzed by GC method and the evaluation was made according to GB 2763—2005. Results The rate of detection for the pesticides was 9.8% (465/4 725)and the disqualified rate was 1.2%(57/4 725).There was a significant seasonal difference in the detection rate of pesticides (P

Objective A large number of traditional Chinese medicine(TCM)are widely used for the treatment of patients with cerebral hemorrhage in China. The aim of this study is to systematically review the existing clinical evidences on TCM treatment for cerebral hemorrhage. Methods Randomized controlled trails(RCTs) of TCM treatment of cerebral hemorrhage were identified, eligible studies were included, the methodological quality of inclusive trails was assessed by the modified Jadad scale. The Cochrane Collaberation’s Revman 5.20 was used for data analysis. Results 69 RCTs were available and included. Meta-analysis indicated that relative risk of overall effective rate of Sanqi, Ciwujia, Chuanxiongqin and Naoxueshu were significant difference; SMD(95% CI) of neural function defect score was SMD=-0.46, 95%CI(-0.56,-0.35)of Sanqi, Danshen, Qingkailing, Liangxuetongyufang;SMD(95%CI) of the reduce of cerebral hemorrhage was SMD = -0.98, 95% CI(-1.32, -0.63)of Danshen, Dahuang, Ciwujia, Qingkailing, Liangxue-Tongyufang. Conclusions The evidence currently available showed that the TCM which included do not increase the death rate and adverse reaction of the patients with cerebral hemorrhage, TCM could reduce neurological deficit and improve the absorption of hematoma.

Objective To explore the effect of compound Xueshuantong capsule Dendrobium luminous pill combined with amiotide eye drops in the treatment of vitreous opacity.Methods According to the digital table, 120 patients with vitreous opacity were divided into two groups,60 cases in each group.The control group was given conventional amiotide eye drops treatment,and the observation group was given compound Xueshuantong capsule Den-drobium luminous pill for treatment on the basis of conventional therapy.The clinical effect was observed and com-pared after treatment.Results The total effective rate in the observation group was 66.67%,which was significantly higher than 38.33% of the control group(χ2 =9.09,P <0.05).The total effective rate of young patients in the obser-vation group was 78.79%,which was significantly higher than 51.85% of the elderly patients(χ2 =8.24,P <0.05). The vitreous opacities total effective rate of the observation group after treatment within two years was 58.33%,which was significantly higher than 28.33% of the control group(χ2 =11.67,P <0.05).Conclusion Compound Xue-shuantong capsule Dendrobium luminous pill combined with ammonia iodine peptide drop has a positive effect in the treatment of ocular fluid of vitreous opacity,improve the clinical total effective rate,recovery of affected vision,and avoid the recurrence of vitreous opacity after operation,has the advantages of safety,effectiveness,which should be widely applied in clinic.

As part of medical scientific spirits and closely associated with medical ethics,medical rational spirit is the spiritual foundation of medical treatment activities and thus should evolve compatibly with medical science.Due to the specific characteristics of medical science,a noble and steady medical rational spirit is an important factor in accelerating harmonious medical consumption and basic request for the application of logic inevitability of medical purpose.

That sparkplugs and cognizances caution of medical morality base line is a realistic proposition that to spurn mammonism phenomenon filters medical service industry and enhances diathesis of medical affairs personnel.Medical service incited by mammonism phenomenon forms a menace to the medical base line,it is important inducement of medical action of getting out of line,and duties of medical risk and complaints of medical comsumer.Mammonism phenomenon form a nubbin quoted by medical morality base line and duties of medical risk.

Objective To construct pcDNA3 1(+)GDNF recombinant eukaryotic expression plasmid and to investigate its expression in eukaryotic cells. Methods The coding sequence of GDNF was amplified from rat astrocytes by reverse transcription PCR (RT PCR) and cloned into pcDNA3 1(+) eukaryotic expression vector The recombinant pcDNA3 1(+)GDNF plasmid was then transfected into eukaryotic cells mediated by using Fu Gene 6 method Analysis by restricting enzyme digestion and DNA sequencing were carried out to demonstrate the sequence of the plasmid GDNF protein and its activity were then determined using pcDNA3 1(+)GDNF plasmid transfected eukaryotic cells Results RT PCR product is 640 bp specific segment Analysis by restricting enzyme digestion and DNA sequencing of pcDNA3 1(+)GDNF recombinant showed results from restricting enzyme were 640 bp and 300 bp segments respectively DNA sequencing revealed that GDNF cloning was successful The recombinant plasmid can express active GDNF protein in eukaryotic cells Conclusion The study on the role of both GDNF and gene therapy is significant in the treatment of Parkinson disease

The main purpose of this study is to observe and analyze the characteristics and the clinical significance of long-term echocardiogram data in patients after mechanical aortic valve replacement operation. Fifty-five cases were recruited over 5 years after mechanical aortic valve replacement operation, with complete echocardiogram examination profiles. The 55 subjects were divided into predominate aortic stenosis group and predominate aortic regurgitation group, based on preoperative examinations. And another way of division was also carried out that according to the effective orifice area index (EOAI), the subjects were divided into three groups, i. e. , severe prosthesis-patient mismatch (PPM) group, moderate PPM group, and non PPM group. Characteristics of long-term echocardiogram data of the subjects were analyzed. Patients with aortic stenosis showed significantly decreased thickness of intraventricular septum and left ventricular posterior wall about half a year after operation (P < 0.05). Half a year after operation, patients with aortic regurgitation showed decreased left ventricular internal dimension diastole and left ventricular internal dimension systole, and increased ejection fraction (P < 0.05). Differences in mean aortic valve pressure gradient, forward blood flow across the aortic valves, and ejection fraction were not significant among those with severe PPM, moderate PPM, and non PPM groups (P > 0.05). Number of cases of ascending aorta dimension increased as time goes on and pathogenesis of other valve diseases. A conclusion could be drawn that the heart function of patients with aortic valve disease could be improved after mechanical aortic valve replacement operation, but pathologies in other valves and ascending aorta might be increased, and therefore periodical postoperative echocardiogram evaluation with long-term pharmaceutical therapy could be necessary.
Aortic Valve Stenosis ; surgery ; Echocardiography ; Heart Valve Prosthesis ; Heart Valve Prosthesis Implantation ; Heart Ventricles ; anatomy & histology ; Humans ; Postoperative Period ; Systole ; Treatment Outcome

The neurovascular unit (NVU) consists of neurons,gllal cells and microvessels,etc.There were complex interactions among the various cellular components,which form as a uniform entirety preferably.Anong the various cellular components of NVU,the signal transduction occurred disorders during cerebral ischemia,resulting in neuronal and extracellular ratrix destruction,and thus occurring cerebral infarction and hemorrhagic transformation.Therefore,the treatment of ischemic sttroke should be based on the protection of the overall function of NVU.This article describes the changes and protection of NVU after cerebral ischemia.

Heparanase (Hpa) is the only β-D-glucuronidase of degading heparan sulfate proteoglycans in the body of mammalian.Studies have confirmed that Hpa accelerates angiogenesis in multiple physiopathological processes; however there are still a few studies about the expression and role of Hpa after cerebral ischemia.This article mainly introduces the relation between Hpa and angiogenesis after cerebral ischemia.