The microsporidia are obligate intracellular eukaryotic parasites.They have been paid more attention as being the emerging pathogen of human, so it is important to control microsporidiosis using fast and precise detecting technology.In order to provide a reference for controlling microsporidian infection effectively, this paper reviews the progress of studying on the detecting technology from the microscopic staining methods, immunological and molecular biology.

Objective To observe the clinical effect of nitroprusside,dopamine and dobutamine for treatment of refractory heart failure. Methods 60 patients with refractory heart failure were randomly divided into control group and treatment group. Clinical symptoms, signs, echocardiography and other changes were observed. Results The total effective rate was 86.67% ,control group was 66.67% ,the total effective between the two groups was statistically significant(P ＜ 0.05). After treatment, left ventricular ejection fraction (LVEF) improved significantly compared with the control group, the difference was statistically significant(P ＜ 0.05). Conclusion The Sodium nitroprusside, dopamine and dobutamine for treatment of refractory heart failure was safe and effective method.

Objective To explore the feasibility of a novel technique of anvil placement in combined use of laparoscopic and thoracoscopic Ivor Lewis esophagectomy. Methods From June 2011 to October 2012, we performed a novel technique of minimally invasive Ivor Lewis esophagogastrectomy in 20 patients with midthoracic esophageal cancer.The anvil was placed to connection with the esophageal stump under thoracoscopic vision, and an apical pleural stomach esophagus anastomosis was performed by using normal stapling. Results The mean operation time lasted for 200 minutes (150-300 minutes) with a mean blood loss of 180 ml (150-250 ml) without blood transfusion.The mean number of lymph nodes resected was 18 (12-44).No additional incision was required. There were no complications at post-operation and no anastomic leakage.The patient’s hospital stay lasted a mean of 10 days (8-12 days) .Follow-up reviews were conducted for 6 months and no recurrence was found in all the 20 patients. Conclusion Laparoscopic and thoracoscopic Ivor Lewis esophagectomy with novel technique of anvil placement for the treatment of esophageal carcinoma ensures the feasibility and safety.

Objective To analyze the results of blood lipids examination in medical workers,so as to understand the cause of ab-normal blood lipid level and formulate the strategy of health education.Methods 500 cases of hospital workers were enrolled as subjects for the study.Under fasting conditions,the samples of the upper arm vein blood were collected,and the detection of total cholesterol(TC),triglycerides(TG),low-density lipoprotein(LDL),and high density lipoprotein(HDL)of serum were proceed on automated analyzers.The results were analyzed.Results In all the 500 workers,there were 152 cases with hyperlipoidemia,ac-counting for 30.4%.The dyslipidemia rate of male workers was 34.7%,and which of female workers was 24.4%.≤30 age group had the lowest dyslipidemia rate(12.9%),and >45-50 age group had the highest dyslipidemia rate(45.8%).There was signifi-cant difference between them(P <0.01).Conclusion The dyslipidemia rates of workers are different across age and gender.It is helpful for workers to have focused health education to improve their live qualities.

Objective To evaluate MRI features of tubal ovarian cysts,to improve the understanding of its imaging characters. Methods 1 5 patients with tubal ovarian cysts proved by pathology and surgery were analyzed retrospectively.Results Among the 1 5 cases with cysts,9 cases were solitary lesions,including the right side of the attachment area in 5 cases and the left side of the at-tachment area in 4 cases.6 cases were multiple lesions located on both sides.The diameters of these cysts were from 3.8 cm to 1 1.0 cm,and average diameter was 5.6 cm.All cases showed that the cysts were round,communicated with fallopian tube.All tubals showed different degrees of expansion.The signals of the cysts showed hypointense on T1 WI and hyperintense on T2 WI.The signals in two cases were complex.After contrast medium administration,the walls of the cysts could be enhanced with different de-grees.Conclusion MRI is useful in displaying the morphology,signal and the wall of the tubal ovarian cysts.

In this paper the development of a new Braille writing board on which raised Braille characters are written is introduced. Compared with traditional Braille writing board on which hollow Braille characters are written, the advantage of new one is convenient for studying, writing and proofreading because the order of Braille in writing is the same as the order of Braille in remembering.

In this paper a series of gait quantitative assessment indexes:phase symmetry index,verti-cal force symmetry index,equilibrium function index,and brake and drive function indexes are obtainedwith VICON 3-dimensinal movement analysis system according to the results that 40 amputees,50 cerebralpalsy patients, 31 hemiplegia patients and 19 paraplegia are evaluated and the differences between the nor-mal and the abnormal(p<0.001)found with these indexes. The quantitative assessment methed of walk-ing function not only provides rehabilitation level of patients and for checking therapeutical effects,but alsolaies the foundation of gait analysis to standardization and practicalization further.

Objective: Finding the essence and regular pattern of the construction of male urine collection devices, and setting up its basic concepts. Methods:Selecting typical construction examples, then analyzing them singly and as a whole. Results:Setting up concepts such as construction module, construction factor, construction factor unit, construction index, construction array etc., and giving out the table of construction factor units. Conclusion:This research has provided mode, methods, means for understanding and further analyzing, studying male urine collection devices.

Objective:To study the correlation of the ratio and number of involved lymph nodes and the prognosis of gastric carcinoma.Methods: 174 patients of positive gastric lymph nodes were included.They were treated by radical gastrectomy and lymph nodes dissection(D 2 or D 3)in our department.The ratio and number of metastastic gastric lymph nodes were calculated.Their influence and their interrelation with pathogenic parameters were analyzed.Results: The overall 5-year survival rate was 29.6%,and the five-year survival rate of 1～20 per cent and more than 20% were 70.6%,12.0% respectively.One to five positive lymph nodes and five more were 46.6%,17.4% respectively.The five-year survival rate was decreased as the ratio and number of involved lymph nodes increased.Studies also indicated that with the increase of depth of cancer invasion,the ratio and number of involved lymph node elevated.Conclusion: The results shows that the ratio and number of involved lymph nodes correlated to tumour progression and the five survival rate.Perigastric lymph node status can be considered as a simple and effective prognosis factor.