[Objective]To investigate whether RNA interference(RNAi)could suppress the expression of HER-2 in osteosarcoma OS-732 cells.[Method]OS-732 cells were transsfected using chemically synthesized double stranded RNA(dsRNA)formulated with Lipofectamine 2000.The HER-2 gene expression inhibition was assessed by RT-PCR,Western Blot and immunocytochemistry.The chemosensitivity of transfected cells to cisplatin was determined by MTT measurement.[Result]Sequence specific siRNAs targeting HER-2 down-regulated the expression of HER-2 significantly.Currently,a range of effects on cell physiology,such as growth inhibition or apoptosis,was observed,Introduction of the siRNA into HER-2 positive tumor lines in vitro greatly reduced the expression of the HER-2 mRNA,effects lasted for 10 days,On the 9th day of interference,HER-2 protein expression was down-regulated in interference group.After RNAi,HER-2-siRNA increased thesensitivity of OS-732 to cisplatin by 10-fold.[Conclusion]Sequence specific siRNAs targeting HER-2 can suppress the expression of HER-2,and significantly inhibiting cellular proliferation,increasing the sensitivity of OS-732 to eisplatin.The successful application of HER-2 siRNA for inhibition of proliferation in HER-2 overexpressed cells extends the list of available therapeutic modalities in the treatment of human osteosarcoma.

Executive Function ; Humans ; Prefrontal Cortex ; physiology

Objective To study on the changes of the IL-6、IL-8、TNF-? in the type 2 diabetes with insulin resistance and the relation of plasma glucose or insulin resistance and cytokines. Methods (1) To assay the serum level of cytokines in 90 cases with type 2 diabetes mellitus (T2DM) and 30 normal controls. (2)To measure the level of fasting plasma glucose (FPG),hemoglohin A_1c (HBA_1c),fasting insulin(FINS),fasting C-peptide (FCP).(3) To analysis the relation of the level of FPG,HBA_1c,FINS,FCP and cytokines. Results (1) The serum levels of cytokines in T2DM group were significantly increase than in control groups,IL-6 and TNF-? in T2DM group with insulin resistance were significantly increase than in T2DM group without insulin resistance (2) There was significantly positively correlated between insulin resistance and the increasing of cytokine levels. Conclusion There is an excess activation of cytokines in T2DM,which is significantly correlated with insulin resistance. Cytokines play the important role in the occurrence and development of T2DM and insulin resistance.

Objective To evaluate the clinical manifestation and the treatment strategy of the scoliosis associated with Chiari malformation and /or syringomyelia. Methods Fifty-two cases suffered from scoliosis with Chiari malformation and /or syringomyelia were divided into three groups for surgical treatment: 1) Group 1: 18 cases had scoliosis with Chiari Ⅰmalformation and /or syringomyelia without obvious neurologic impairment, their scoliosis was corrected with posterior instrumentation, but their Chiari Ⅰmalformation and syringomyelia were left untreated surgically. 2) Group 2: 12 patients, whose scoliosis was not indicated for surgery but those Chiari malformation associated with syringomyelia, underwent posterior sub-occipital craniectomy to enlarge foramen occipital magnum, C1 posterior arch decompression, duraplasty and syrinx-subarachnoid space shunting no matter whether neurologic deficits were present or not. 3) Group 3: 22 cases in addition of correction of scoliosis, due to neurologic deficits caused by Chiari Ⅰmalformation or syringomyelia, two-stage surgery was indicated: firstly, with posterior suboccipital craniectomy to enlarge foramen occipital magnum, C1 posterior arch decompression, duraplasty and syrinx-subarachnoid space shunting 6 months later, then underwent the scoliosis correction with instrumentation. Results In 34 patients who underwent craniovertebral decompression, only 6 of the 24 cases with preoperatively neurologic deficits achieved mild improvement within 6 months postoperatively. In 40 patients who were treated with posterior correction for scoliosis, the average frontal correction was 63% and the average sagittal correction was 80% for scoliosis less than 90?, the average frontal correction was 49% and the average sagittal correction was 74% for scoliosis more than 90?. At a follow-up of 6 months to 5 years, the average loss of the frontal correction was 6%. Conclusion Scoliosis associated with Chiari malformation and /or syringomyelia can be effectively treated with the similar results, as for adolescent idiopathic scoliosis. The mainstay for diagnosis is MR imaging. Accurate diagnosis and proper treatment for Chiari malformation or syringomyelia before scoliosis surgery will improve the rate of scoliosis correction, decrease the neurological complications.

Objective To evaluate the biomechanical basis and long term clinical outcome of the offset hook-screw combined instrumentation in preventing against correction loss after it is used to treat the unstable thoracolumbar fractures. Methods Of 98 cases treated with the offset hook-screw combined instrumentation from July 1997 to December 2002, 64 with follow-up over two years were reviewed. All 64 cases received operation within average 3.2 days after injury, of which 41 were fixated with CD or CD-Horizon instrumentation and 23 with TSRH instrumentation. The long term clinical outcome evaluation included the maintenance of the vertebral height restoration, the sagittal kyphotic Cobb angle loss and the coronal Cobb angle correction loss. Results No death occurred. But after operation, there was one case with worsened neurological deficits (recovered one week after surgery) and two with deep infection. The average post-operative vertebral height and the coronal Cobb angle were restored by 89.0% and 100.0% respectively and the sagittal kyphotic Cobb angle corrected from preoperative 31?to postoperative -1? . During the follow-up for 24-62 months, no instrumentation breakage occurred. The pseudoarthrosis was affirmed in one case and suspected in two with the vertebral height loss of 8.6% and the kyphotic Cobb angle correction loss of 4?respectively. Conclusions The offset hook-screw combined instrumentation can satisfactorily restore the normal sagittal profile, effectively prevent the fixation loosening or breakage, minimize the mechanical failure of instrumentation, and particularly reduce the loss of long-term correction.

[Objective] To evaluate the surgical techniques and its clinical results of CDH Legacy in the treatment of scoliosis.[Method]Nine patients(7 female and 2 male)with scoliosis instrumented with posterior CDH Legacy system were recruited for this retrospective study.The etiological classification of scoliosis were idiopathic for 7,congenital for 1,neurofibromatosis(NFl)for 1.The average age was 13.5 years(ranged 11～18 years).The average preoperative Cobb's angle was 54?(ranged 48?～68?).Seven AIS and one CS patients received one stage posterior CDH Legacy instrumentation;the NFl patients received one stage anterior epiphysiodesis and second stage posterior CDH Legacy instrumentation.[Result]There was no death,no infection and no neurological complication.No intra-operative fracture,no dura matter or pleuml laceration occurred.Superior Mesenteric Artery Syndrome occurred in one patients and recovered with conservative treatment.Posterior Cobb's angle averaged 20? with correction rate of 63%.The duration of follow-up averaged 23 months(ranged 20～30 months).Surgical time averaged 260 min(ranged 210～300 min)and mean EBL was 700 ml(300～1 000 ml).Bony fusions were achieved in all patients and no coronal or sagittal plane decompensation occurred.The loss of correction at last follow-up was 4? with 7.4% loss of correction rate.[Conclusion]The clinical results of CDH Legacy are as good as the other three generation implants,its advantage lies in lower profile,solid inter-locking,versatile implants and handy manipulation.

There were no data concerning restenosis rate after percutaneous transluminal coronary angio-plasty available in China so far. Our study was focused on clinical and angiographic follow-up and tried to reveal the predictive value for angiographic restenosis using noninvasive tests among 592 patients whose 841 coronary arteries were dilated in our department from Dec. 1987 to Dec. 1994.This patient group consisted of 503 males and 89 females had average age of 58. 4 ? 9. 1. The overall success rate was 93. 3% and acute vessel complications was 4. 1%. After successful procedures of PTCA,the patients were followed up with recurrence of angina, Holler ECG, submaximal excercise ECG and 99mTc-MIBI exercise scintigraphy regularly. 62 of these patients underwent coronary angiographic follow-up from 4-24 months (average time: 10. 4 ? 7. 6 months) after PTCA. We found 35 patients with angiographic restenosis and 27 patients without. Thus, we divided the patients into restenosis group and non-restenosis group according to the angiographic results. Subsequently, we oberserved whether the symptom and noninvasive tests were valuable in prediction of restenosis. The result showed that recurrence of angina, Holter ECG, submaximal exercise ECG and 99mTc-MIBI scintigraphy were useful in detecting restenosis with their specificity and sensitivity 75.7% and 72.0%, 71. 4% and 86. 7%, 66. 7% and 73. 1% ,76. 5% and 83. 3 %, respectively. Moreover, If any two of above four ischemic indexes were combined together to detect restenosis,the specificity and sensitivity were significantly enhanced (85. 2% and 92. 9%). It is concluded that recurrence of angina and the noninvasive tests were valuable in predicting restenosis. The combination of these ischemic indexes would greatly improve their predictive value.

OBJECTIVE To investigate the impact on semen quality of Ureaplasma urealyticum(Uu) and Chlamydia trachomatis(Ct) infections.METHODS Uu and Ct in semen were detected and the samples of semen were analyzed for 228 cases in infertiliry group and 132 cases in fertility group,The semen quantity,sperm density,survival rate,vitality,liquefacient duration,normal morphologic rate,deformity rate and death rate were compared.RESULTS Among 228 cases in infertility group,173 were positive in Uu and Ct(75.9%),obviously higher than that of fertile males(28.0%).Sperm density,vitality,survival rate and normal morphologic rate in the infertility group were remarkably lower than those of the fertility group(P0.05).CONCLUSIONS The genital infection of Uu and Ct has its impact on the multiple parameters of semen,and semen quality as a whole is decreasing.

Objective To discuss the use of anterolateral thigh flap for the repair of large defects of soft tissues in dorsum of the foot.Methods A total of 38 cases of dorsal skin defects of the foot was repaired with the anterolateral thigh flap containing the lateral femoral cutaneous nerves.Corresponding vessels and sensory nerves were anastomosed. Results The flaps survived in all the cases.The sensation in dorsum of the foot recovered completely within 2~4 months after operation and the touch,pain,and temperature sensation of the donor area recovered within 3~6 months after operation.Follow-up observations in the 38 cases for 4~18 months(mean,9 months) showed flaps with normal color,texture,and appearance.Conclusions It is recommended to repair large-scale defects in dorsum of the foot with nerved anterolateral thigh flap.

0.05). No death, pseudoarthrosis, or implantation failure occurred. Conclusions One-row portal pattern is suitable for patients with long-segment deformity and short anteroposterior thoracic diameter, while double-row portal pattern is suitable for patients with short-segment deformity and long anteroposterior thoracic diameter.