1.How we diagnose and treat chronic lymphocytic leukemia.
Chinese Journal of Hematology 2018;39(7):529-532
4.Neutropenic Hematological Malignancy Patients with Imipenem-resistant Pseudomonas aeruginosa Sepsis:Analysis of 3 Cases
Jingyan XU ; Jian OUYANG ; Qiguo ZHANG ; Yong XU ; Chaoyang GUAN
Chinese Journal of Nosocomiology 2009;0(15):-
OBJECTIVE To investigate the clinical characteristics of imipenem-resistant Pseudomonas aeruginosa sepsis in neutropenic hematological malignancy patients. METHODS A retrospective review of the medical records of 3 cases in neutropenic hematological malignancy patients with P.aeruginosa sepsis in June 2008 were taken. RESULTS All they died within 3 days,2 times blood culture results indicated.With imipenem-resistant P.aeruginosa growth. CONCLUSIONS Neutropenic hematological malignancy patients with imipenem-resistant P.aeruginosa sepsis have rapid progress of the disease and high mortality rates,we should step up surveillance.
7.Expression of WWOX protein and its significance in non-small cell lung cancers.
Yu-long ZHOU ; Yong-jian XU ; Zhen-xiang ZHANG
Chinese Journal of Oncology 2007;29(4):297-297
Adenocarcinoma
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metabolism
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pathology
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Adult
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Aged
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Carcinoma, Non-Small-Cell Lung
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metabolism
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pathology
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Carcinoma, Squamous Cell
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metabolism
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pathology
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Female
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Humans
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Immunohistochemistry
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Lung
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chemistry
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pathology
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Lung Neoplasms
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metabolism
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pathology
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Male
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Middle Aged
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Oxidoreductases
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metabolism
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Sex Factors
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Smoking
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Tumor Suppressor Proteins
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metabolism
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WW Domain-Containing Oxidoreductase
8.Optimization of whole-cell biocatalysis for phenylacetyl- 7-aminodeacetoxycephalosporanic acid production.
Jinheng FU ; Jian ZHAO ; Baixue LIN ; Yang XU ; Yong TAO
Chinese Journal of Biotechnology 2014;30(11):1781-1785
Cephalosporins are widely used antibiotics owing to their broad activity spectra and low toxicity. Many of these medically important compounds are made chemically from 7-aminodeacetoxycephalosporanic acid. At present, this intermediate is made by synthetic ring-expansion of the inexpensive penicillin G to form G-7-ADCA, followed by enzymatic removal of the side chain to obtain 7-ADCA. The chemical synthetic process is expensive, complicated and environmentally unfriendly. Environmentally compatible enzymatic process is favorable compared with chemical synthesis. In our previous research, metabolic engineered Escherichia coli strain (H7/PG15) was constructed and used as whole-cell biocatalyst for the production of G-7-ADC with penicillin G as substrate. The whole-cell biocatalysis was studied by single factor experiment, including the composition of substrates and the conversion conditions (OD600, pH, concentration of penicillin G, MOPS, glucose, time and FeSO4). After optimization, 15 mmol/L of G-7-ADCA was obtained. The process is convenient, efficient and economic. This work would facilitate the industrial manufacturing and further product research.
Anti-Bacterial Agents
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biosynthesis
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Biocatalysis
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Cephalosporins
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biosynthesis
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Escherichia coli
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metabolism
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Metabolic Engineering
9.Advances in minimal residual disease detection with flow cytometry in childhood acute myeloid leukemia.
Jian-hua FENG ; Xiao-jun XU ; Yong-min TANG
Chinese Journal of Pediatrics 2013;51(3):231-234
Adolescent
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Child
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Child, Preschool
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Clinical Trials as Topic
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Flow Cytometry
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Hematopoietic Stem Cell Transplantation
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Humans
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Immunophenotyping
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Leukemia, Myeloid, Acute
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diagnosis
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pathology
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therapy
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Neoplasm, Residual
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diagnosis
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pathology
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therapy
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Polymerase Chain Reaction
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Prognosis
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Recurrence
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Sensitivity and Specificity
10.Comparison with Several Methods to Isolate Epiphytic Bacteria from Gracilaria lemaneiformis (Rhodophyta)
Yong-Jian XU ; Guan-Zong LE ; You-Ping ZHANG ;
Microbiology 1992;0(01):-
We used 4 methods,such as ultrasonic crush(UC),ultrasonic rinse(UR),whorl surge(WS)and rubbing(RU),to isolate epiphytic bacteria from red alga Gracilaria lemaneiformis.Then,we counted bacteria numbers,detected bacterial species,observed bacterial configuration and characteristic of cell wall.Compared with these methods and with different treatments in one method,the results were drawn:the UR and RU were inferior in all methods to isolate bacterial numbers and species,the UC and WS were better,especially,the treatment 30W 30s of UC was the best in experiment,which isolated 12 of 16 bacterial species,and got 1.75 10~6 cells per gram wet weight G.lemaneiformis.