1.Advance in the role of MicroRNAs in leukemia
International Journal of Pediatrics 2010;37(3):311-313
MicroRNAs(miRNAs)are a series of short non-coding RNAs that can regulate the expressions of protein-coding genes posttranscriptionally.They modulate gene expression by targeting mRNA for degradation or blocking translation via base-pairing to complementary sites in the 3'-untranslated region of the target mRNAs.Their functions are related to many biology progresses and diseases including tumor and hematopoietic differentiation.They are speculated to be critical in the development, progression and prognosis of leukemia. In this review, we will summarize the research progress on miRNAs in leukemia.
2.Human infection due to Streptococcus suis.
Chinese Journal of Epidemiology 2005;26(9):645-648
3.Clinical observation of the glaucoma trabeculectomy with tunnel knife making the double-deck scleral flap
Min, FU ; Qian, YU ; Yong-Chun, ZHUANG
International Eye Science 2015;(7):1197-1200
AlM: To study the trabeculectomy clinical effect of use tunnel knife to make double - deck scleral flap and to cut off the layer scleral flap of glaucoma.METHODS: Using the random grouping method to divide 46 cases (60 eyes) of glaucoma into the treatment group of 24 cases (32 eyes) and control group of 22 cases (28 eyes). The treatment group, tunnel knife was used to make double- deck sclera flap and superficial scleral flap about the size of 5mm×5. 5mm, 1/3 scleral thickness, under the sclera flap made another one about the size of 3. 5mm× 4mm, 1/3 scleral thickness, resected the middle layer of the sclera flap, removed 2mm×2mm trabecular tissue, underwent routine peripheral iridectomy, could adjust suture the superficial scleral flap, sutured Ball fascia and bulbar conjunctiva. ln control group, routine glaucoma trabeculectomy was undergone.RESULTS:Patients were followed up for 1a, the vision in treatment group was obviously better than that in the control group, with a statistically significant difference (P< 0. 05). The postoperative intraocular pressure of the two groups of patients were significantly lower than that of the preoperative one. Postoperative 1 and 3mo, no statistical significant difference of intraocular pressure in two group(P>0. 05). But after 6 and 12mo, the intraocular pressure of the treatment group were significantly lower than that of the control group, with statistically significant difference (P<0. 05). Postopeartive 1a, the cumulative complete success rate and conditions for successful rate were 90. 63% and 96. 88% in the treatment group, and those were 75% and 89. 29% in control group. There was significant difference between two groups(P<0. 05).CONCLUSlON:The trabeculectomy have a good effect to lower the intraocular pressure by use tunnel knife to make double-deck scleral flap and to cut off the layer scleral flap. The scleral flap have uniform thickness, smooth surface, and the function of the filtering bleb maintained for a long time, less postoperative complications, suitable for various types of glaucoma, so it is worthy of clinical promotion.
4.Acupuncture for acute urticaria.
Cheng ZHONG ; Yong-Zhen ZHUANG
Chinese Acupuncture & Moxibustion 2013;33(2):108-108
Acupuncture Points
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Acupuncture Therapy
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Adolescent
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Adult
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Child
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Female
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Humans
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Male
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Middle Aged
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Treatment Outcome
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Urticaria
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therapy
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Young Adult
5.Anesthetic efficacy of epidural ropivacaine mixed with chloroprocaine for hysterectomy
Zhuang WANG ; Yong-Hao YU ; Qing LI ;
Chinese Journal of Anesthesiology 1994;0(06):-
Objective To assess the efficacy of the mixtures of different concentrations of ropivacaine(R) and chloroprocaine(C)for epidural anesthesia in patients undergoing hysterectomy.Methods Sixty ASAⅠorⅡpatients aged 27-56 weighing 45-75 kg undergoing elective hysterectomy were randomly divided into 4 groups(n= 15 each);groupⅠ0.75% R alone;groupⅡ0.5% R-1% C;groupⅢ0.5% R-1.5% C and groupⅣ0.75% R+1% C.The epidural catheter was placed at L_(2,3)interspace and advanced 3.5 cm into the epidural space in cephalad direction.A total of 22 ml of epidural solution was injected in each group.The onset time,block height and duration of sensory block and the onset time,degree and duration of motor block(using Bromage scale)were assessed.The use of supplementary drugs(ketamine and ephedrine)and side effects were recorded.Results The onset time of sensory and motor block was significantly shorter in groupⅡ,ⅢandⅣthan in groupⅠ(0.75% R alone).The duration of sensory and motor block was significantly shorter in groupⅡandⅢthan in groupⅠandⅣ.The incidence of hypotension was significantly increased but the incidence of discomfort produced by traction on the viscera during operation was reduced in groupⅣas compared with groupⅠ.Conclusion The anesthetic efficacy of epidural 0.5% ropivacaine is significantly enhanced when mixed with 1.0% or 1.5% chloroprocaine.
6.Experimental study on inhibitory effect of wogonin on proliferation and invasion of breast cancer cells.
Kai-Fei HUANG ; Yuan ZHUANG ; Yi-Qi HUANG ; Yong DIAO
China Journal of Chinese Materia Medica 2014;39(8):1485-1489
OBJECTIVETo study the inhibitory effect of wogonin on the growth and proliferation of breast cancer cells MDA-MB-23, and observe its effect on the adhesion, migration and invasion of MDA-MB-23 cells, in order to further study its molecular mechanism.
METHODMTT assay was used to detect the effect of wogonin on MDA-MB-23 cell growth. Ki-67 assay was adopted to test the effect of wogonin on cell proliferation. Scratch test, adherence test and invasion chamber assay were taken to detect the effect on the migration and invasion abilities of MDA-MB-231 cells. Proliferation and metastasis-related proteins and relevant signaling pathways were detected by Western blotting.
RESULTWogonin could remarkably inhibit the growth and proliferation of MDA-MB-231 cells, significantly inhibit migration, adhesion and invasion abilities of breast cancer cells at a low concentration, and effectively inhibit the expression of Survivin, Bcl-2, ICAM-1, MMP-2, MMP-9 proteins of MDA-MB-231 cells.
CONCLUSIONWogonin could notably inhibit growth and proliferation of breast cancer cells, and inhibit migration, adhesion and invasion of MDA-MB-231 cells. Its invasive and adhesive effects on MDA-MB-231 cells may be related to the decrease in ICAM-1, MMP-2, MMP-9 expressions.
Breast Neoplasms ; genetics ; metabolism ; pathology ; physiopathology ; Cell Line, Tumor ; Cell Movement ; drug effects ; Cell Proliferation ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Female ; Flavanones ; pharmacology ; Gene Expression Regulation, Neoplastic ; drug effects ; Humans ; Intercellular Adhesion Molecule-1 ; genetics ; metabolism ; Matrix Metalloproteinase 2 ; genetics ; metabolism ; Matrix Metalloproteinase 9 ; genetics ; metabolism ; Neoplasm Invasiveness ; Signal Transduction ; drug effects
9.Integrated e-clinical solutions in clinical research.
Charles YAN ; Xian-qiang MI ; Yong-long ZHUANG
Acta Pharmaceutica Sinica 2015;50(11):1393-1395
Implementation of information technology in clinical research has resulted in revolutionary changes in drug development. Based on the good clinical practice (GCP) requirements on data, processes and documentations, and the era of fast growth in clinical studies using up-to-date information technology, we explore an integrated e-clinical solution in clinical studies in China.
China
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Clinical Trials as Topic
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Data Collection
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methods
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Medical Informatics
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methods
10.Progresses in molecular biologic studies on coagulase negative staphylococcus infection.
Jian-hui DI ; Xu-zhuang SHEN ; Yong-hong YANG
Chinese Journal of Pediatrics 2004;42(1):26-29
Bacteremia
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etiology
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Catheterization
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adverse effects
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Child
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Coagulase
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metabolism
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Cross Infection
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etiology
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Drug Resistance, Bacterial
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drug effects
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Endocarditis, Bacterial
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etiology
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Humans
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Methicillin
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pharmacology
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Quinolones
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pharmacology
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Staphylococcal Infections
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complications
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drug therapy
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microbiology
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Staphylococcus
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classification
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drug effects
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pathogenicity
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Urinary Tract Infections
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etiology
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Vancomycin
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pharmacology