1.Tualang and Kelulut Honey Reduced Lipopolysaccharidesstimulated Inflammatory Responses of Mic
Low Zhao Xuan ; Tan Kai Li ; Yong Yoke Keong ; Looi Chung Yeng ; Ooi Yin Yin
Malaysian Journal of Medicine and Health Sciences 2023;19(No.3):375-379
Microglial activity is crucial in maintaining the central nervous system (CNS) homeostasis. However, prolonged
microglial activation have been implicated in the pathology of neurodegenerative diseases. Activated microglia will
increase the production of inflammatory cytokines, reactive oxygen species (ROS) and alter their surface marker
expression levels. This study used Malaysian honey, Tualang honey (TH), and Kelulut honey (KH) to determine lipopolysaccharide (LPS)-stimulated inflammatory responses of microglia. TH and KH at 0.1% were used in the current
study as our findings showed no significant difference in the cell viability between BV2 cells treated with 0.1 % of
TH and KH and control group. TH and KH reduced the ROS level significantly by 41.62±1.06% and 49.16±0.63%,
respectively, and slightly reduced the expression of co-stimulatory molecules, CD40 and CD11b in LPS-activated
BV2 cells. Our preliminary findings proposed an in-depth future study on the anti-inflammatory effect of TH and KH
on microglial activation.
2.Evaluation of Anti-Hyperlipidaemic Activity of a Mixture of Zinger officinale, Allium sativum, Citrus Lemon, Honey, and Malus domestica Vinegar (ZACAH) Extracts in Rats Fed with High Cholesterol Diet
Kokila Vani Perumal ; Hasnah Bahari ; NorShafarina Shari ; Kasturi Kanniappan ; Khairul Kamilah Abdul Kadir ; Zunoliza Abdullah ; Mohd Radzi Ahmad ; Ibrahim Kalle Kwaifa ; Siti Hadizah Jumidil ; Azrina Zainal Abidin ; Sabariah Md Noor ; Yong Yoke Keong ; Santhra Segaran Balan
Malaysian Journal of Medicine and Health Sciences 2022;18(No.5):55-63
Introduction: The incidence and complications of hyperlipidemia associated co-morbidities and mortalities are
grossly increasing globally. Intervention with natural products preparation has been identified as an alternative for
the management of hyperlipidaemia and its related cardiovascular complications. This study investigated the anti-hyperlipidaemic activities of a mixture of Zinger officinale, Allium sativum, Citrus lemon, honey, and Malus domestica
vinegar (ZACAH) extracts in in Sprague Dawley (SD) rats fed with high cholesterol diet. Methods: Thirty-six male
SD rats were randomly distributed into 6 groups, including normal chow diet-fed (NC), high cholesterol diet (HCD),
HCD+ Simvastatin (standard drug) while the remaining three groups were fed with HCD + ZACAH extracts at different doses (1, 3 and 5mg/kg body weight) for 18 weeks. Simvastatin at 10 mg/kg of bodyweight was used as control.
High-performance liquid chromatography (HPLC) was used to determine phenolic compounds present in ZACAH
extracts, elastase inhibitory assay was determined using spectrometric with a substrate (N-Succ-(Ala)3-pnitroanilide
(SANA) while 2,2-diphenyl-1-picrylhydrazyl (DPPH) was performed based by the method described by Blois, 1958.
Results: In vitro; ZACAH extracts had oxygen radical absorbance capacity (ORAC) value of 2000 µmol TE/100 mL,
total phenolic content (TPC) of 7537 ± 54.5%, DPPH free radical scavenging activity of 27.34 ± 2.71%, elastase
inhibitory assay of 29.29 ± 1.65% and lipoxygenase inhibitory assay of 98.58 ± 1.42%. In vivo, ZACAH extracts
showed decreased bodyweight, adipose tissue, improved lipid profiles and hepatic biochemical enzymes. Conclusion: These results suggested that ZACAH extracts supplementation improved hyperlipidaemia in SD rats and might
be a promising adjuvant for the treatment of hyperlipidaemia.