Objective To observe the clinical efficacy on primary biliary cirrhosis(PBC) treated by two methods. Methods 50 cases with PBC were divided into the combination therapy group(A group:n =25) and simple western medicine treatment group(B group:n = 25) ;The curative effect and indicators of liver function were observed before and after treatment in patients with PBC. Results A total effective in group A 19 cases(76.0%) were higher than in B group 15 cases (60.0%) (x~2 = 3.87, P ＜ 0.05) ;The clinical symptoms and signs in A、B group after treatment were better than before treatment(x~2 = 3. 87 ～ 6. 89,P ＜ 0.05 ～ 0.01) ;The clinical symptoms and signs in A group after treatment were better than B group(x~2 = 3.88 ～ 4. 12. ,all P ＜0.05) ;The indicators of biochemical in A、B group after treatment were lower than before treatment(t = 2. 214 ～ 2. 517, P ＜ 0.05); The indicators of biochemical of ALP、GGT、ALT in A group decreased significantly compared with B group(t = 2. 284 ～ 2. 418, P ＜0.05). Conclusion Integrative Chinese and western medicine achieves good clinical efficacy in patients with PBC.

Objective To study the clinical efficacy of hypertension with nephropathy treated by integrated Chinese and Western medicine. Methods 75 patients with hypertensive nephropathy were divided into the integrated Chinese and Western medicine group (group A) and 35 cases of pure western medicine treatment group (group B) ; The change of whole blood viscosity at low shear rate(ηbL)、 whole blood viscosity at high shear rate( ηbH)、fibrino-gen( Fg)、 serum albumin(Alb)、hemoglobin(Hb)、urine prottein( Up)、blood urea nitrogen( BUN) 、creatinine( SCr)、 creatinine clearance rate( CCr)、residual renal function index ( RRFI) before treatment and after treatment were observed. Results The effective rate 31 cases(88.5% ) in group A was significantly higher than the B group 23 cases (65.7%)(x2 =3.98,P<0 05);The contents of ηbL、ηbH、BUN、Scr、CCr and RRFI in treatment group after treatment had significant difference wmpared with that before treatment(t = 2. 121,t = 223,t =2. 324,t = 2. 375,t = 2. 371 ,t =2. 265 ,t = 2. 415 ,t =2.771 ,P <0.05) ;The contents of Up、BUN、Scr、CCr、RRFI were significantly different in two groups after treatment(t =2.331 ,t = 2.378,t =2.411,t =2. 378,t =2.391 ,P <0.05). Conclusion Integrated Chinese and Western medicine for hypertensive nephropathy had good efficacy,was a safe and effective method.

Child ; History, 20th Century ; History, 21st Century ; Humans ; Nephrology ; history ; Pediatrics ; history ; Societies, Medical ; history

Abnormalities, Multiple ; diagnosis ; pathology ; physiopathology ; Branchio-Oto-Renal Syndrome ; diagnosis ; pathology ; physiopathology ; therapy ; Child ; Deafness ; etiology ; physiopathology ; Diagnosis, Differential ; Ear ; abnormalities ; Female ; Humans ; Kidney ; abnormalities ; Renal Insufficiency ; etiology ; physiopathology ; therapy

Humans ; Immune System Diseases ; diagnosis ; etiology ; therapy ; Wound Infection ; immunology

Objective To explore transthyretin (TTR) effect on retinal vascular endothelial cells (hREC) under high glucose and hypoxia environment.Methods hREC and human retinal pigment epithelial cell (hRPEC) were cultured at low-glucose (LG),high glucose (HG) and hypoxia.The glucose concentration was increased from 5.5 mmol/L up to 25 mmol/L,and hypoxia was induced by 200 μmol/L CoCl2.The cells were divided into LG group,LG-hypoxia group,HG group,HG-hypoxia group according to the different cell culture environment.The growth index was detected at 0,4,8,16,24,36,48,60,72 hours after cultured.Furthermore,hREC and hRPEC were also cultured with additional TTR (4 μmol/L),respectively.Then transwell co-culture system was employed to reveal the effects of hRPEC on the growth of hREC.Results At 72 hours after cultured,the growth index of hREC and hRPEC in LG group were increased as compared with LG-hypoxia group and HG group (hREC:F=17.098,22.970;P＜0.05.hRPEC:F=45.442,9.011;P＜0.05);the growth index of hREC and hRPEC were decreased in HG group and HG-hypoxia group (hREC:F=146.184,P＜0.05;hRPEC:F=27.907,P＜0.05).Additionally,hREC could be significantly repressed by added TTR during culture with high concentration of glucose (F=161.430,24.106;P＜0.05).hREC could be significantly increased by added TTR during culture with low concentration of glucose (F =200.486,48.662;P ＜ 0.05).In co-culture process,hRPEC revealed inhibition activity against hREC under both natural and abnormal environment (LG group:F=15.711,P＜ 0.05;LG-hypoxia group:F =45.659,P＜0.05;HG group:F =7.857,P ＜0.05;HG-hypoxia group:F=6.348,P＜0.05).Conclusion Under high glucose and hypoxia environment,the growth of hREC from neovascular could be inhibited by TTR.

Metabolic bone disease in nephrotic syndrome(NS) are increasingly being renal physician's attention.As calcium binding protein and VitD binding protein losing with a large number of protenuria, the bone metabolic biochemical abnormalities had happened at the beginning of the onset of the nephrotic syndrome, and is further exacerbated by therapeutic high-dose or long course of glucocorticoids (GC) application.The main mechanism of the glucocorticoid-induced osteoporosis (GIOP) is for GC to inhibit the activity of osteoblasts and promote apoptosis of osteoblasts and formation of osteoclasts, resulting in secondary hyperparathyroidism,leading to increasing the risk of osteoporosis,slow growth and fracture,seriously harm to children's physical and mental health.The biomarkers of bone transform can prompt the NS with bone metabolic abnormalities early;vertebral body bone dual-energy X-ray absorptiometry bone mineral density detection is the best method and position to determine GIOP.As the most commonly used and effective means to prevent and control metabolic bone disease, calcium supplements and VitD were always taken when GC was treated for NS, even the dosage of GC was very low.So far, it is still lack of guideline of prevention and treatment of bone metabolic abnormalities in NS in children.

Objective　To examine the soluble protein patterns of three ordinary species of cockroaches in Anhui province by SDS-PAGE technique, and to find the method of scientific classification for cockroaches. Methods　The soluble protein liquid was made by the way of freezing and melting homogenate. SDS-PAGE was carried out in the liquid. After using Coomassie brilliant blue G250, the molecular weight of all of protein strips were measured and calculated by standard curve. Results　The numbers of female and male of Periplaneta fuliginosa were 31 strips and 29 strips; the numbers of female and male of Periplaneta Americana were 30 strips and 28strips; the numbers of female and male of Blattella germarica were 28 strips and 25 strips. The number and location of protein bands were different among these species. It was also obvious that the positions of the main protein bands in Blattella germarica were different from those in two Periplaneta species, and the positions of the main protein bands in female insect were different from those in male insect. Conclusion SDS-PAGE can be used as one of classification methods for cockroaches.

Calculi ; chemically induced ; Ceftriaxone ; adverse effects ; Child ; Humans ; Lithiasis ; chemically induced

Objective To investigate the running condition of defluoridation projects and fluoride concentration in drinking-water in Anyang City of Henan Province.Methods Cluster sampfing of Anyang,Hua, Neihuang,Tangyin Counties emdimic fluomsis poining of Anyang City was conducted places of different orientation, about 1/3 towns in the selected examination towns had their defluoridation project investigated.Results Among 472 pwjects investigated,427 were running normally,accounting for 90.47%(427/472),only 9.53%(45/472)of the projects were out of service.68.62%(293/427)of the pwjects had normal fluoride concentration in drinking water, 31.38%(134/427)of the projects failed to do 80.In Hua County,41.00%(41/100)of the projects were out of service. In Neihuang County.70.39%(107/152)of the projects had abnormally high fluoride concentration in drinking water. Conclusions Defluoriding projects are out of service and fluoride concentration in drinking water exceeds the standard in Hua,Neihuang Counties of Anyang City.We must analyse the cause of it seriously,work out measures, promote a healthy and sustainable development of defluondation projects