OBJECTIVE:To compare the effects of2different treatment methods on the assaying of triptolide alcohol in tripterygium wilfordii cut crude drug.METHODS:After concentration,tripterygium wilfordii cut crude drug water decoction underwent column chromatographic separation after adsorption with neutral alumina(Method1)and neutral alumina column chromatographic separation after ethanol precipitation and extraction by chloroform(Method2),respectively.Triptolide alcohol was determined by HPLC and the assaying result underwent pairing and t tests.RESULTS:There was significant difference between the2methods with the content determined by Method1significantly higher than that by Method2(P

Thyroid cancer is the most frequent endocrine malignancy. The histologic subtype of thyroid carcinoma is mainly differentiated thyroid carcinoma. The treatment modality of thyroidectomy followed by 131I and thyroxine-suppressive therapy has been approved and applied widely. A brief introduction and a commentary on this topic is presented in this article.

Post-hepatitis cirrhosis (PHC),which results from either hepatitis B virus (HBV) or hepatitis C virus (HCV) infection,and its associated with hemodynamic changes may cause the spleen to become functionally hyperactive-a condition known as hypersplenism.Hypersplenism is associated with anemia,leukopenia,thrombocytopenia and splenomegaly.There are several effective methods to treat hypersplenism with the development of medical technology and the main purpose is the remission of hypersplenism,reducing the portal hypertension and decreasing the risk of hemorrhage.However,complete splenectomy or partial treatment of the hyperactive spleen is still controversial all over the world.This may be caused by the lack of the knowledge on the pathophysiological characteristics and clinical significance of treating hypersplenism.This review is a comprehensive discussion on the recent research which investigates hypersplenism caused by PHC.

Levothyroxine monotherapy for primary hypothyroidism has gratifying effects.Doctor should recommend the optimal timing of taking levothyroxine to patients for individualized therapy according to the medical history,the pharmacokinetic characteristics of levothyroxine,and various factors affecting its absorption.After reaching the therapeutic goal,most of patients could live,study,work,marry,and bear as healthy subjects.

Objective To investigate the ideal methods for correction of lower eyelid retraction by lateral canthal anchoring.Methods The authors retrospectively reviewed the records of 126 patients(207 eyelids) operated on between June 1999 and 2005 by one senior surgeon.The age of the patients ranged from 28 to 72 years(mean,46 years).Transcanrhal canthopexy was used in 20 patients(33 eyes);The 3 mm tarsal strip procedure was used in 35 patients(57 eyes);Transcanrhal canthopexy combined with Hamra's lower blepharoplasty was used in 26 patients(45 eyes).The tarsal strip procedure combined with Hamra's procedure was used in 45 patients(72 eyes).75 patients(123 eyes) were followed up for 6～12 months.Mean follow-up time was 8 months. Results Of them,125 patients were uniformly satisfied with their cosmetic and functional outcome at last follow-up;one patient had undercorrection of retraction.There were no major complications and only one minor complications(corneal irritation from graft sutures),which resolved in the early follow-up period.Conclusions The lateral canthal anchoring is an effective,aesthetic,and functional treatment for moderate to severe lower eyelid retraction resulting from multiple causes.This procedure is associated with predictable results,a low morbidity rate,and high patient satisfaction.

OBJECTIVE:To study the application and development of pedicle screws for the internal fixation of thoracolumbar vertebra fracture and to evaluate the biocompatibility. METHODS:A computer-based online search was conducted in Medline database and CNKI with the OBJECTIVE:To study the application and development of pedicle screws for the internal fixation of thoracolumbar vertebra fracture and to evaluate the biocompatibility. METHODS:A computer-based online search was conducted in Medline database and CNKI with theof "pedicle screws,thoracolumbar,fractures,biocompatibility" in both Chinese and English from 1989 to June 2009. A total of 32 out of 57 articles were included according to inclusion and exclusion criteria to summarize the treatment of thoracolumbar vertebra fracture using pedicle screws and the complication prevention and cure of pedicle screws,and to investigate clinical application,biocompatibility,and prevention of interruption of pedicle screws. RESULTS:Internal fixation of posterior pedicle screws was an effective method to treat thoracolumbar vertebra fracture; however,breakage of pedicle screws was easily caused following internal fixation. Pedicle screws combined with implantation of autogenous bone or artificial materials into cavity of injured vertebra could effectively relieve loosening and interruption of implants; however,the price was expensive. Sufficient bone graft for internal fixation and implant dislodgment following bone fracture healing might effectively prevent interruption of internal fixation device. Pedicle screw intension of stainless steel materials was strong,but the biocompatibility was poor. While,the biocompatibility of titanium materials was great,but the intension was weak,thus the pedicle screws were easily broken. CONCLUSION:Internal fixation of pedicle screws is an effective method to treat thoracolumbar vertebra fracture. However,the biocompatibility still needs to be improved. Titanium pedicle screws have a good biocompatibility but poor intension; however,stainless steel pedicle screws have a good intension but poor biocompatibility.

Objective To explore the effects of siRNA targeting survivin combined with radiotherapy on human carcinoma cells SW480.Methods The human colorectal carcinoma cell line SW480 was treated with siRNA targeting survivin.The expression level of survivin in transfected SW480 cells was detected by RT-PCR and immunocytochemistry.The effects of siRNA on cell cycle were analyzed by FCM with PI staining.Radiotherapy was combined with siRNAs targeting survivin to study the sensitivity of SW480 induced by siRNA targeting survivin to radiotherapy.The effects of the survivin siRNA combined with radiotherapy on the growth of SW480 were studied by MTT assay.The apoptosis rate of SW480 was analyzed by FCM with annexinV/PI staining.Results RT-PCR demonstrated that the expression of survivin was knocked down significantly by the siRNA.Arrest in G2/M phase of the cell cycle was detected by FCM in the SW480 cells transfected with siRNA targeting survivin.The MTT assay showed that siRNA targeting survivin combined with radiotherapy inhibited the growth of SW480 cells more significantly compared with single treatment.More apoptosis cells(almost 35.72%) were detected by FCM in the SW480 cells treated with siRNA combined with radiotherapy.Conclusions The small interfering RNA can inhibit survivin of the human colorectal carcinoma cell line SW480.The siRNA targeting survivin combined with radiotherapy can inhibit the proliferation of SW480 and increase the percentage of apoptotic cells markedly.The siRNA targeting survivin can enhance the sensitivity of colorectal carcinoma cell line SW480 to radiotherapy in vitro.

Circulating cell-free nucleic acids are defined as extracellular DNAs or RNAs in blood with physiological or pathological origins. Previous studies showed that the concentration of cell-free nucleic acids in the blood of cancer patients is significantly higher than in healthy people. Further studies showed that the genetic and epigenetic alterations of circulating cell-free nucleic acids are relevant to cancer development and progression, including mutation, hypermethylation, loss of heterozygosity, change of integrity, and abnormal expression of microRNAs. Detection of circulating cell-free nucleic acids shows promising potential in cancer screening, diagnosis, personalized treatment, and prognosis.

China ; Dental Prosthesis ; Humans ; Tooth Loss ; surgery

Breath Tests ; Humans ; Mining ; Pneumoconiosis ; diagnosis ; Radon ; analysis