Angioplasty, Balloon ; Blood Vessel Prosthesis Implantation ; Humans ; Ischemia ; surgery ; Knee ; blood supply ; surgery ; Treatment Outcome

Objective To evaluate the effect of the assessment of intervention of rational use and special initiative of antineoplastic Chinese patent medicine in tumor ward of Nantong First People’s Hospital (hereinafter referred to as “the hospital”); To facilitate the rational use of medicine in clinical setting. Methods Medical records of discharged patients in April and October 2015 were collected, and various types of irrational use of Chinese patent medicine was analyzed and put into statistical analysis. Results The proportion of medical records of irrational use of Chinese patent medicine declined from 39.45% to 18.49%; the percent of irrational prescription order sheet declined from 11.49%to 3.45%;the differences of variors irrational medicine use before and after the intervention were statistically significant. Conclusion The status of the rational use of antineoplastic Chinese patent medicine in tumor ward is improved after half-year special intervention and correction initiative.

Purpose:To study the efficacy and the side ef fe ct of morphine controlled-release tablets plus diclofenac sodium in the treatme nt of pain of metastatic bone carcinoma. Methods:69 patients with pain were randomly divided into two gr oups. Group Ⅰ was treated only with morphine controlled-release tablets (30 mg ,q 12 h),GroupⅡ with morphine controlled-release tablets (30 mg,q 12 h) and diclofenac sodium (100 mg,q 12 h). The analgesia effect and adverse reaction were observed. Results: Both have analgesia effect,but group Ⅱ was superior (P

Recently,considerable progress has been made in the treatment of systemic lupus erythematosus(SLE),especially in the clinical use of new immunosuppressive agents,which leads to improved remission rate and life quality.However,plasmapheresis still shows to be necessary in severe lupus with serious complications.With the development of plasmapheresis technology,the efficiency and specificity of antibody adsorption being improved significantly,it played an important role in the treatment of acute stage life-threaten lupus.Here we intend to make a review on the relative improvement of plasmapheresis technology and its clinical use.

No abstract available.
Actinomycosis*

Objective To prepare a targeted ultrasound micro bubble,which carried the HSV-TK gene,and investigate the in vitro target searching ability of the micro bubbles and inhibitory effect on the proliferation of HepG2 cells.Methods Ultrasonic micro bubbles were prepared by mechanical vibration method,construction of targeted HSV-TK ultrasound micro bubbles by biotin affinity bridge construction.To detect the general characteristics of ultrasound micro bubbles,and to test its effect on the proliferation of HepG2 cells in vitro.Results HSV-TK targeted ultrasound microbubbles more gathered on the surface of HepG2 cells,through detection of PCNA and MTT,it was found that the proliferation of gene targeting microbubble group was obviously decreased,cell apoptosis increased significantly,Cells invade experiments showed that the number of cells in genetic microbubble group (22.18 ± 2.01) decreased significantly compared with the control group and the nontargeted group,can effectively inhibit the proliferation and invasive ability of HepG2 cells.Conclusion Targeted ultrasound microbubble carrying target gene have better inhibitory effect on HepG2 cells in vitro.

BACKGROUND:The posterior lumbar interbody fusion is one of the effective methods for the treatment of lumbar and sacral spine diseases. Most surgeons fix the cage by compressing the disc space in order to keep stability and prevent dislodgement. However, some surgeons think that the non-compression technique does favor for increasing of the disc and foraminal height and thus improving the clinical outcomes, and does not increase the risk of fusion shift.
OBJECTIVE:To compare the effects in fixing the cage by the compression and non-compression techniques on posterior lumbar interbody fusion.
METHODS:Data of 64 patients with single-segment lumbar degeneration undergoing posterior lumbar interbody fusion between August 2009 and June 2014 were retrospectively analyzed. Fusion device was fixed according to compression of intervertebral space. These patients were divided into compression group (n=30) and non-compression group (n=34).
RESULTS AND CONCLUSION:(1) Curative effects:Lumbar and leg pain visual analogue score,
Oswestry disability index, SF-36 score and the height of intervertebral space, intervertebral foramen height and lumbar lordosis were significantly improved postoperatively in each group compared with preoperatively (P<0.05). The intervertebral space, intervertebral foramen height and lumbar lordosis were significantly better in the non-compression group than in the compression group (P<0.05). (2) Lumbar fusion rate:No significant difference in lumbar fusion rate was detected 6 and 12 months after surgery and during final fol ow-up (P>0.05). (3) Correlation analysis:The increase of the intervertebral space and the height of the intervertebral foramen were not significantly correlated with the improvement of the clinical curative effect (P>0.05). (4) Test results demonstrated that outcomes of the compression technique to fix the cage are equivalent to the non-compression in posterior lumbar interbody fusion. Non-compression is advantageous to increase the intervertebral space and the height of the intervertebral foramen. Both of them are conducive to the recovery of lumbar lordosis, but are not correlated with the increase in clinical curative effect.

Objective To clone, express and purify human PIF1 protein and analyze its ATPase activity. Methods The PIF1 cDNA was amplified by PCR from HeLa cell cDNA library and inserted to pET24b with histidine tag at its terminus to form pET24b-PIF1 plasmid. The recombinant pET24b-PIF1 plasmid was transformed to RosettaTM 2 (DE3) and the expression of PIF1 protein was monitored by SDS-PAGE analysis. By using fast protein liquid chromatograph (FPLC) system, the PIF1 protein was purified by affinity chromatograph and gel filtration. The ATPase activity of PIF1 was checked by thin layer chromatograph(TLC). Results The PIF1 protein was successfully cloned and expressed in E.coli. Conclusions The purification procedure of PIF1 protein was established using FPLC. The overexpressed and the purified PIF1 helicase has DNA and Mg2+ dependent ATPase activity.

Animals ; Breast Neoplasms ; metabolism ; Humans ; NF-kappa B ; metabolism ; Neoplasms ; metabolism ; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma ; metabolism ; Proto-Oncogene Proteins c-bcl-2 ; metabolism ; Receptors, Notch ; metabolism ; physiology ; Signal Transduction

Objective To investigate the effects of high volume hemofiltration(HVHF) on cardiopulmonary functions and IL-?plasma levels in lung injury models. Methods Sixteen dogs with oleic acid-induced lung injury were randomly assigned into two groups(A and B). Group A served as control, undergoing mechanical ventilation only. Group B received HVHF and mechanical ventilation. Hemodynamic and respiratory mechanic variables were measured before and 0 mm, 120 mm, 240 mm after the induction of lung injury in both groups. Blood and ultrafiltrate samples were collected at the same time points. Plasma and ultrafiltrate concentrations of IL-? were measured by EUSA. Results There were significant differences between two groups on hemodynamic values for mean pulmonary arterial pressure(mPAP), pulmonary artery wedge pressure(PAWP), pulmonary vascular resistance(PVR) and mean arterial pressure(mABP) (P