Objective:To evaluate the therapeutic effect of combined procedure of surgical left ventricular repair (LVR) and skeletal myoblast transplantation for congestive heart failure after myocardial infarction. Methods: Forty-eight male adult Lewis rats, with a four-week old myocardial infarction created by left coronary artery ligation, were randomized into 4 groups: group Ⅰ (n=10) received phosphate-buffered saline (PBS) injection into the border zone of the infarction; group Ⅱ(n=11) received myoblast injection ; group Ⅲ(n=13) received left ventricular repair and simultaneous PBS injection; and group Ⅳ (n=14) received left ventricular repair and myoblast injection. Echocardiography was performed once a week until the fourth week after the treatment. Results: Left ventricle end diastolic diameter (EDD) and end systolic diameter (ESD) declined significantly one week after the LV repair in group Ⅲ and Ⅳ, then increased as time passed; on the fourth week, there were no statistical differences between group Ⅰ,Ⅱ and Ⅲ, but group Ⅳ was still much smaller than the other 3 groups(P

BACKGROUND:Recent studies have shown that platelet rich plasma has strong osteogenic ability and it has been widely used in the field of department of orthopedics and department of stomatology, but experimental study on avascular necrosis of the femoral head has been not reported.
OBJECTIVE:To observe the effect of platelet rich plasma gel combined with marrow core decompression in the repair of aseptic necrosis of rabbit femoral head.
METHODS:A total of 25 New Zealand white rabbits were obtained. Without dislocation of the hip joint, aseptic necrosis model was established by using liquid nitrogen cryotherapy on bilateral femoral head of rabbits. At 2 weeks after model establishment, rabbit models were randomly assigned. Model group with nine rabbits did not receive any treatment. Core decompression group with eight rabbits underwent core decompression treatment. Combination group with eight rabbits underwent core decompression combined with platelet rich plasma gel treatment. At 8 weeks after model induction, tissue sections of femoral head specimens were col ected and subjected to MRI and hematoxylin-eosin staining.
RESULTS AND CONCLUSION:(1) MRI examination:The model group showed low signal area of different forms in femoral head fat high signal, exhibited annular, ribbon and focal shapes. No evident changes in osteogenesis, no apparent minification of the defect cavity were detected at defect site in the core decompression group, showing long T1 and T2 signals. Defect cavity disappeared, and new bone of short T1 and T2 signals fil ed in the combination group. (2) Hematoxylin-eosin staining:Articular cartilage defect and repair existed in the model group, showing osteoarthritis-like changes. Empty bone lacuna of bone trabecula, hematopoietic celland adipocyte necrosis in bone marrow were detected in the core decompression group. Empty bone lacuna of bone trabecula, focal necrosis scattered in bone marrow were visible in the combination group. Results suggested that the outcomes of platelet rich plasma gel combined with core decompression were better than core decompression alone in treatment of rabbit models of femoral head necrosis.

Objective To observe the effect of laparoscopy combined with choledochoscopy in treatment of cholelithiasis clinical effect .Methods 80 cases diagnosed as cholelithiasis cases were divided into control group and observation group with 40 cases in each group .The control group used the traditional laparotomy incision gallbladder stone ;observation group were treated by laparos-copy combined with choledochoscopy .Results In the observation group ,the operation and the hospital stay were significantly shor-ter than the control group ,operation curative effect was obviously superior to the control group ,the incidence of postoperative com-plications was significantly lower than the control group .Conclusion The use of laparoscopy combined with choledochoscopy tech-nology in treatment of gallstones have good clinical effect .

Nucleic acids, as a next generation of biotechnology drugs, not only can fundamentally treat diseases, but also own significant platform characteristics in view of technology and production. Therefore, nucleic acid-based drugs have broad clinical applications in biomedical fields. However, nucleic acids are degradable and unstable, and have very low intracellular delivery efficiency in vitro and in vivo, which greatly limits their applications. In recent years, ionizable lipid-based lipid nanoparticles have shown promising application potentials and have been successfully applied to COVID-19 (Coronavirus Disease 2019) vaccines in clinic. Lipid nanoparticles demonstrate high in vivo delivery efficiency and good safety profile due to their unique structural and physicochemical properties, which provides many possibilities for their clinical applications for nucleic acid delivery in the future. This review focused on the characteristics of nucleic acid drugs and their delivery barriers, and discussed the approved nucleic acid drugs to illustrate the key aspects of the success of their delivery carrier system. In addition, problems to be solved in the field were highlighted.

Acupuncture Therapy ; Child, Preschool ; Gait Disorders, Neurologic ; etiology ; physiopathology ; therapy ; Hand, Foot and Mouth Disease ; complications ; virology ; Humans ; Male ; Walking

Atrial Fibrillation ; surgery ; Catheter Ablation ; methods ; Humans

BACKGROUND:Clinical treatment of epicondyle fractures and intercondylar comminuted fractures is quite tricky due to instability and spread to the articular surface. Common complications contained bone delayed union, nonunion, broken nails and broken boards. Internal fixation for fractures is various, but reasonable choice for clinical fixation plays a decisive role for repair of fractures.
OBJECTIVE:To explore the repair effects of various fixation methods on femoral condyle fracture from different aspects such as fracture type, bone healing, functional recovery and biomechanics.
METHODS:First author searched PubMed database and China National Knowledge Infrastructure for articles about various fixation methods in repair of femoral condyle fracture published from January 2000 to April 2014. Key words were“femoral condyle fracture, internal fixation, biomechanics, load-displacement, axial stiffness, horizontal shear stiffness”. Total y 142 articles were retrieved, but 39 articles met the inclusion criteria.
RESULTS AND CONCLUSION:Femoral condyle fracture caused the damage to normal anatomic structure of knee join, changed normal anatomical axis and the mechanical axis of knee joint. Therefore, anatomic reduction and rigid internal fixation are necessary. During treatment, the advantages and disadvantages of various fixation methods should be known. It is necessary to ful y evaluate the type of intercondylar fractures of the femoral condyle and soft tissue injury. According to biological characteristics of the human body and mechanical property, internal fixation device should be reasonably used. Individual treatment programs should be provided. Thus, the occurrence of postoperative complications should be reduced utmostly, resulting in a satisfactory repair outcome.

objective to find a proper pathway for the regional chemotherapy of pancreatic carcinoma. Method A total of 39 cases of unresectable pancreatic carcinoma was collected and divided into five groups. Ⅰ: celiac artery group, 5 cases; Ⅱ: gastroduo-denal artery group,24 cases;15 cases were directly inserted into this artery (ⅡB)and 9 cases indirectly through gastroepiploric artery (ⅡA). Ⅲ: gastroepiploric right artery group, Scases; Ⅳ: splenic artery group, 5 cases. Result. For these groups, the first group can be detected that the pancreatic head is clearly dyed; in the second group the pancreatic head was apparently dyed. The body and tail of pancreas were clearly dyed in the splenic artery group and failed in the second group. Conclusion For pancreatic head carcinoma, the gastroduodenal artery is the first choice; splenic artery is proper for the carcinoma in the body and tail of pancreas.

Mouse AtlasTM cDNA Arrays were used for comparative studies of gene expression in mouse embryonic fibroblasts (MEF) before and after in vitro cultivation and 24 types of differentially expressed genes were identified. To screen and identify the differentially expressed new genes of MEF, cDNA suppression subtractive hybridization (SSH) technique was used. The cDNAs derived from cultured MEF after SSH were subcloned into pGEM T easy vectors to set up the subtractive library, transfected into E. coli strain JM109, sequenced and analyzed with bio informatics methods after PCR amplification.Altogether 42 types differentially expressed genes and 3 novel ESTs were identified and categorized from total 128 ESTs derived from cultured MEF after SSH. It is suggested that the genes expressed by MEF were the important molecular basis supporting the survival, proliferation and self renewal capacities of embryonic stem cells.

Objective To investigate the effects of rutaecarpine on the cerebral injury and calcitonin gene-related peptide (CGRP) level during focal cerebral ischemia-reperfusion injury in rats. Methods Rats were intravenously given three doses of rutaecarpine (50, 100 and 300?g/kg) or vehicle 30 minutes before experiment. Then the rats subjected middle cerebral artery occlusion (MCAO) for 2 hours, and neurological deficits scores were performed at 6, 12 and 24 hours after reperfusion. After the last test the animals were sacrificed, the infarct volumes were determined by TTC staining, and CGRP levels were measured by radioimmuoassay. Results Rutaecarpine significantly reduced infarct volume, and improved cerebral function in dose-dependent manner compared with vehicle. Rutaecarpine significantly inecreased brain CGRP levels after reperfusion as well. Conclusion Rutaecarpine has marked protective effects on ischemic brain damage in rats possibly by increasing CGRP release in the brain.