Objective: To study the genes associated with CQ(Chloroquine) increased radiosensitivity in radioresistant MDA-MB 231 cells.Methods: The survival curve of MDA-MB231 breast cancer cells was establish in the presence or absence of CQ after irradiation by colongenic assay.The relationship between certain protein expressions(MLH1,ERCC1,PARP,RAD51,XRCC4) and CQ increased radiosensitivity in radioresistant MDA-MB 231 cells was examined by Western blot method.The genes which are associated with CQ increased radiosensitivity were studied by microarray analysis.Results: CQ increased deaths of parental MDA-MB231 cells,radiosensitive MDA-MB231 cells and redioresistant MDA-MB231 cells were compared with those of the CQ-untreated control cells after irradiation,and the Radiation Potentiation Factor were 1.21-1.23.The expression of ERCC1 in the presence and absence of CQ in the radioresistant cells was significantly higher than that of sham-irradiated control cells by Western blot method.After irradiation,RAD51 level was increased up to 3-hour radiation and then gradually reduced.The expression of MLH1 was lower in 24 hours than that of other time points after irradiation.There were no differences on the expressions of MLH1,ERCC1,RAD51 in cells with and without CQ,which suggested that these genes weren't correlated with CQ increased radiosensistivity.XRCC4 and PARP levels were not changed by irradiation.Microarray analysis showed that TNP3,FRIH,CA21,ADTA genes might be related to CQ increased radiosensistivity in radioresistant cells.Conclusion: CQ can increase radiosensistivity in radioresistant MDA-MB231 breast cancer cells.It is not related to MLH1,ERCC1,PARP,RAD51,XRCC4 genes.(TNP3) may play an important role in CQ increase radiosensistivity in radioresistant MDA-MB231 breast cancer cells.

Objective To evaluate whether ABCD2 score can discriminate dizziness patients with and without stroke. Methods This retrospective case-control observational study was conducted in 403 hospitalized patients. According to the final discharge diagnosis, the patients were divided into two groups:the stroke group and non-stroke group. The areas un-der the receiver-operator curves and 95%confidence intervals were then generated to estimate the diagnostic value. Re-sults ABCD2 score was higher in stroke group than in non-stroke group. Conclusions ABCD2 score can be used to quickly identify dizziness patients with cerebrovascular diseases.

Objective To determine the proportion and the clinical value of the thymus extending superiorly into the lower neck in children.Methods The MR images of 400 normal children were retrospectively reviewed.According to the childhood staging criteria.all children were divided into four groups:the infancy and toddlerhood group(0-3 yo,42),the pre-school age group(>3-6 yo,87),the school age group(>6-12 yo,168),and the adolescence group(>12-18 yo,103).The presence or absence of the superior extension of the thymus above the manubrium Was determined on sagittal T2-weighted images.If so,the distance between manubrium and thymic apex was measured.The associations between frequency of ccrvicaI extension and age and sex were evaluated using correlation analysis.Results Superior extension of the thymus was detected in 288(288/400,72.0%)children,with 39(39/42,92.9%)in the infancy and toddlerhood group,72(72/87,82.8%)in the pre-school age group,118(118/168,70.0%)in the school age group,and 59(59/103)in the adolescence group(57.3%).There Was a statistically significant relationship between age group and frequency of cervical extension of the thymus (X2=4.9684,P<0.05).There was no significant decreasing trend in the mean distance of cervical extension between age increasing groups(F=2.28,P>0.05).Conclusions Cervical extension of the thymus occurs in the majority of children,and should be considered as a normal finding.Through recognizing this finding,doctors can avoid misdiagnosing it as a pathologic mass.

OBJECTIVETo detect the levels of miR-499 and relative proteins in hearts of mice after exercise training, and investigate the mechanism of exercise-regulative apoptosis.

METHODSMale C57BL/6 mice were randomly divided into 3 groups( n = 14): sedentary (SE), exercise training 1 (ET1) and exercise training 2 (ET2) group. SE did not do any exercise. ET1 performed swimming training for 8 weeks. ET2 performed the same work as ET1 until the 5th week. Then, mice trained twice a day until the end of training. TUNEL assay was applied to test myocardial apoptosis, RT-PCR and Western blot were used to detect miR-499 and proteins levels respectively.

RESULTSCompared with SE, stress in ET1 failed to affect apoptotic index (AI) and miR-499-CaN-Drp-1 pathway (P > 0.05). In contrast, exercise load in ET2 increased miR-499 level, decreased Drp-1 level and AI with statistical significance respectively (P < 0.05), but neither CaN expression nor CaN activity was changed significantly (P > 0.05).

CONCLUSIONSwimming training can inhibit myocardial apoptosis, and the decrease in Drp-l may be responsible for the reduced myocardial apoptosis. CaN, the upstream protein, does not participate in exercise-regulative apoptosis.

Animals ; Apoptosis ; Dynamins ; metabolism ; Heart ; Male ; Mice ; Mice, Inbred C57BL ; MicroRNAs ; metabolism ; Mitochondria, Heart ; physiology ; Myocardium ; pathology ; Physical Conditioning, Animal ; Swimming

Epidermal growth factor receptor (EGFR) is necessary in normal epithelial cell growth. Ab-errant EGFR expression is linked with increased cell proliferation and decreased apoptosis. Mutations and over-expression of EGFR are common features of many cancers. Therefore, Therapeutic agents that target the EGFR signal pathway, such as small-molecule tyrosine kinase inhibiiors and monoclonal antibodies are now advanced in clinical development. EGFR-targeted therapies of non-small-cell lung cancer (NSCLC) have been studied as a new way.

This paper reviewed some new types of skin whitening agents and their mechanisms of action from aspects of suppressing melanogenesis, reducing melanosome transference and enhancing desquamation of melanin pigment from the epidermis etc.

Purpose:Expression of MEK-1 was studied by regulating ?V integrin and E-cadherin on MCF-7 breast cancer cells.Methods:Using cell transfected techniques site-mutated genes were inserted into MCF-7 cells and made MCF-7 cells containing MEK-1-GFP,Ala 222 MEK-1-GFP,Asp 222 MEK-1-GFP ,respectively. These cells were tested with immunoblot analysis and immunofluoresence technique to determined the change of MEK-1 expression and location of MEK-1 in cells.Results:Deactivated Ala 222 MEK-1-GFP inhibited the activity of MAPK,active Asp 222 MEK-1-GFP increased the activity of MAPK. Ala 222 MEK-1-GFP increased the expression of E-cadherin,did not increase the expression of ?V integrin and inhibited FAK phosphorylation. MEK-1-GFP and Asp 222 MEK-1-GFP did not affect the expression of E-cadherin ,but upregulated the expression of ?V integrin,increased FAK phosphorylation.Conclusions:MEK-1 regulated the expression of ?V integrin and E-cadherin,FAK is the key factor that induced signal pathway of ?V integrin. It is possible that MEK-1 regulated FAK phosphorglation by regulating expression of ?V integrin and E-cadherin.

AIM: To investigate the application of atomic force microscope (AFM) in clinic diagnosis. METHODS: Topographic images and some parameters of large range field and microstructures of erythrocytes in the blood of normal subjects, lung cancer and myelodisplastic syndrome (MDS) patients were examined by atomic force microscope. RESULTS: Many clear topographic images of many erythrocytes, single erythrocyte, and microstructure of erythrocyte membrane surface were obtained. Many erythrocytes in lung cancer patients were found to change into echinocytes. One erythrocyte had 10-20 protuberances, most of which, with a mean width of 589.0 nm and a length of 646.7 nm, were on the edge of cells. The protuberances on the center of echinocytes are lodged and embedded. The erythrocytes of MDS patients were biconcave in shape. Many apertures with different diameters of tens to hundreds nanometer appeared on the surface of cell membrane. CONCLUSIONS: AFM can be widely applied in clinic pathological inspection, including quantification of cells, obtainment and comparison of many parameters (such as diameter, thickness, volume, surface, surface area/volume ratio), observation of topograph of single cell, and observation and comparison of membrane surface microstructure of cells, and so on.

Objective To evaluate the effects of peer education program on HIV/AIDS prevention among senior high school students. Methods Three senior high schools were randomly selected from Hongkou District of Shanghai as sample schools. One hundred and forty-three students from one class of each school were randomly selected as intervention group,and 152 students from another class of each school were served as control group. Six class hours of peer education program on HIV/AIDS prevention were conducted among intervention group. The same questionnaires which contained knowledge of reproductive health,HIV/AIDS and sexually transmitted diseases were administered before the program,one week and one year after the program. Results The knowledge scores before the program,one week and one year after the program in intervention group were 21.66?8.71,31.72?7.78 and 30.97?8.46,respectively,and those in control group were 22.32?9.24,22.61?8.66 and 22.51?9.16,respectively. There was no significant difference in the knowledge scores before the program between intervention group and control group,while there were significant differences in the knowledge scores one week and one year after the program between intervention group and control group (P

Organ transplantation relies on organ donation,which is an 'altruistic behavior'.In order to sever the shortage of organ donors we should develop citizen education which is correlative to it,The mode and methods of citizen education on organ donation includes: education by schools and society,edifying and typical examples,self-education and internalized morality.