Fracture Fixation ; Fracture Healing ; Humans ; Medicine, Chinese Traditional ; Radius Fractures ; therapy

Objective:To determine the prognostic value of DNA ploidy and nuclear morphometry,and its relations to nuclear grading in renal cell carcinoma.Methods:Pathological grading were done in 47cases of renal cell carcinoma in accordance with the system proposed by Fuhrman et al.Feulgen staining were performed in the tumor histological slides,and the technique of image cytometry was used to quantitate nuclear DNA ploidy and nuclear morphological parameters.The results combined with the data of clinical follow up were analyzed.Results:The mean DNA ploidy in grade Ⅰ～Ⅱ was mainly 2C,≥5C cells less than 10%;the 2C cells in grade Ⅲ～Ⅳ were lower than in grade Ⅰ～Ⅱ,≥5C cells were more than 20%.Among the four grades DNA ploidy were statistically different( P

Objective To investigate the feasibility of bedside temporary transvenous cardiac pacing with balloon tipped floating catheter under non X-ray fluoroscopy.Methods Twenty-four patients of bedside temporary transvenous cardiac pacing,11 cases underwent common electrode (common group),13cases underwent balloon tipped floating electrode (balloon tipped group).The operating time,pacing thresholds,pacing perception and complications were observed between two groups.Results All balloon tipped group placement success,8 cases in common group successfully.The operating time in balloon tipped group was significantly less than that in common group [(2.5 ± 1.5) min vs.(9.3 ±5.3) min],there was significant difference (P ＜ 0.05),but pacing thresholds and pacing perception between two groups had no significant difference (P ＞ 0.05).Common group occurred complications in 2 cases,respectively cardiac perforation and induced paroxysmal ventricular tachycardia,balloon tipped group occurred concurrent electrode wire dislocation in 2 cases.Conclusion Compared with the common electrode,balloon tipped floating electrode is simple,fast,high success rate and low risk of complications,suitable emergency physician bedside operating emergency temporary pacing.

80) via portal vein.After reperfusion 1 h,1 d,3d and 7 d respectively,the concentration of superoxide dismutase(SOD) and catalase(CAT) were tested,and the apoptosis of hepatocarcinoma also was observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL) method.Results The results indicated that the SOD concentration in both hepatocarcinoma tissue and normal hepatic tissues decreased following I/R and perfusion with hyperoxic fluid liquid.The concentration of CAT increased following I/R in normal hepatic tissues.In hepatocarcinoma tissue,concentration of CAT decreased after reperfusion for 1 d and reached its lowest point.After perfusion with hyperoxic fluid,the concentration of SOD in both hepatocarcinoma tissue and normal hepatic tissues decreased more quickly following I/R and the low level was still found on 7 d after reperfusion.The concentration of CAT in tissues of both groups decreased and reached the lowest level at 1 h after reperfusion,but it was restored at 3 d reperfusion in normal hepatic tissues,and in hepatocarcinoma tissue was still at lower level until 7 d after reperfusion. After I/R,the apoptotic cells increased in normal hepatic and hepatic cancer tissues,and were most marked in tissues of hepatic carcinoma at 1 d and 3 d after perfusion with hyperoxic fluid.After I/R and perfusing with hyperoxic fluid,the changes of SOD and CAT and apoptosis in hepatocarcinoma tissue were more obvious than that in normal hepatic tissues(P

The diversity of diagnostic criteria of idiopathic thrombocytopenic purpura (ITP) makes it difficult to compare clinical trial results and exchange clinical experiences. To address this issue, an ITP international working group convened a consensus conference in Italy in October 2007, and some new consensus concerning the terminology, definition, phases, grading of severity, prognosis, and treatment were achieved. The treatment of ITP has been dramatically improved along with the introduction of novel therapeutic agents. Rituximab, a monoclonal anti-CD20 antibody that is able to deplete autoantibody-producing B lymphocytes, has been widely applied because of its high efficacy and safety. Recent evidences suggest that decreased platelet production may also contribute to the development of ITP. Therefore, novel thrombopoiesis-stimulating agents such as thrombopoietin-receptor agonists Romiplostim and Eltrombopag have become new therapeutic options for ITP.
Humans ; Purpura, Thrombocytopenic, Idiopathic ; diagnosis ; therapy

OBJECTIVETo detect the levels of miR-499 and relative proteins in hearts of mice after exercise training, and investigate the mechanism of exercise-regulative apoptosis.

METHODSMale C57BL/6 mice were randomly divided into 3 groups( n = 14): sedentary (SE), exercise training 1 (ET1) and exercise training 2 (ET2) group. SE did not do any exercise. ET1 performed swimming training for 8 weeks. ET2 performed the same work as ET1 until the 5th week. Then, mice trained twice a day until the end of training. TUNEL assay was applied to test myocardial apoptosis, RT-PCR and Western blot were used to detect miR-499 and proteins levels respectively.

RESULTSCompared with SE, stress in ET1 failed to affect apoptotic index (AI) and miR-499-CaN-Drp-1 pathway (P > 0.05). In contrast, exercise load in ET2 increased miR-499 level, decreased Drp-1 level and AI with statistical significance respectively (P < 0.05), but neither CaN expression nor CaN activity was changed significantly (P > 0.05).

CONCLUSIONSwimming training can inhibit myocardial apoptosis, and the decrease in Drp-l may be responsible for the reduced myocardial apoptosis. CaN, the upstream protein, does not participate in exercise-regulative apoptosis.

Animals ; Apoptosis ; Dynamins ; metabolism ; Heart ; Male ; Mice ; Mice, Inbred C57BL ; MicroRNAs ; metabolism ; Mitochondria, Heart ; physiology ; Myocardium ; pathology ; Physical Conditioning, Animal ; Swimming

Ankle sprains are orthopedic clinical common disease, accounting for joint ligament sprain of the first place. If treatment is not timely or appropriate, the joint pain and instability maybe develop, and even bone arthritis maybe develop. The mechanism of injury of ankle joint, anatomical basis has been fully study at present, and the diagnostic problem is very clear. Along with the development of science and technology, biological modeling and three-dimensional finite element, three-dimensional motion capture system,digital technology study, electromyographic signal study were used for the basic research of sprain of ankle. Biomechanical and kinematic study of ankle sprain has received adequate attention, combined with the mechanism research of ankle sprain,and to explore the the biomechanics and kinematics research progress of the sprain of ankle joint.
Ankle Injuries ; physiopathology ; Ankle Joint ; physiopathology ; Biomechanical Phenomena ; Finite Element Analysis ; Humans ; Sprains and Strains ; physiopathology

Objective To investigate the occurence of glomus tumor and investigate its diagnosis and treatment. Methods 13 cases of glomus tumors were analyzed retrospectively. There were 9 females and 4 males. All 13 cases were surgically treated under megaloscpe. Results All the cases were testified as glomua tumors by pathology. There was no recurrence during the follow up of 6 months to 1 year. All the patients were symptom free. Conclusion Glomus tumor has specific features on clinical presentation. Mierosurgieal excision under megaloscpe is fill elective method.

Objective:To explore the therapeutic value of atorvastatin combined bezafibrate on adjusting lipid in pa‐tients with acute coronary syndrome (ACS) .Methods :A total of 121 ACS patients who were treated in our hospital from Apr 2013 to Sep 2013 were enrolled .According to therapeutic method ,they were divided into bezafibrate group (n=60 ,received bezafibrate treatment based on routine treatment ) and combined treatment group (n=61 , received additional atorvastatin based on treatment of bezafibrate group ) .Levels of total cholesterol (TC) ,triglyc‐eride (TG) ,low density lipoprotein cholesterol (LDL‐C) and high density lipoprotein cholesterol (HDL‐C) ,stand‐ard‐reaching rate of blood lipid and incidence rate of adverse reactions were compared between two groups before and after treatment .Results:Before treatment ,there were no significant difference in all blood lipid levels between two groups , P> 0.05. Compared with before treatment ,levels of TC ,TG and LDL‐C significantly reduced and HDL‐C level significantly rose in both groups after treatment , P< 0.05 or < 0.01 ;Compared with bezafibrate group ,there were significant reductions in levels of TC [ (4.06 ± 0.49) mmol/L vs .(3.35 ± 0.29) mmol/L] ,TG [ (1.60 ± 0.33) mmol/L vs .(1.12 ± 0.21) mmol/L] and LDL‐C [ (2.18 ± 0.39) mmol/L vs .(1.91 ± 0.26) mmol/L] ,and significant rise in HDL‐C level [ (1.09 ± 0.21) mmol/L vs .(1.26 ± 0.26) mmol/L] ,P<0.05 all;and sig‐nificant rise in standard‐reaching rates of TC (41.67% vs .67.21% ) ,TG (61.67% vs .85.25% ) ,LDL‐C (36.67%vs .70.49% ) and HDL‐C (41.67% vs .90.16% ) in combined treatment group after treatment ,P<0.05 all .There was no significant difference in incidence rate of adverse reactions (11.48% vs .10.00% ) between combined treat‐ment group and bezafibrate group , P>0.05. Conclusion:Atorvastatin combined bezafibrate can significantly im‐prove blood lipid condition and possess good safety in ACS patients .

Objective:To observe the clinical treatment effects of Tangkang Xifang in the patients with type 0 diabetes foot ( DF) . Methods:Totally 73 patients with type 0 DF were randomly divided into the treatment group (n=37) and the control group (n=36). All the patients discontinued other medications for 7d before the treatment. Base on the blood glucose control with the traditional treat-ment by metformin hydrochloride (1. 5 g·d-1), the treatment group was additionally given warm water foot bath with Tangkang Xi-fang, while the control group was additionally given warm water foot bath, 30 min per time and twice a day for 60 days. The changes in the clinical symptoms, ankle brachial index ( ABI) , motor nerve conduction velocity ( MCV) and some other indices were measured before and after the treatment. Results:In comparison with the control group (58. 3%), the treatment group had higher total effective rate (86. 5%), and the usage of Tangkang Xifang also significantly improved the physiopathologic syndromes of peripheral artery dis-ease and neuropathy (P<0. 01). The ABI and MCV in the treatment group were significantly better than those in the control group (P<0. 05 or P<0. 01). Conclusion:Tangkang Xifang can effectively improve the clinical symptoms of type 0 DF, which is suitable for the clinical application.