Objective:To determine the prognostic value of DNA ploidy and nuclear morphometry,and its relations to nuclear grading in renal cell carcinoma.Methods:Pathological grading were done in 47cases of renal cell carcinoma in accordance with the system proposed by Fuhrman et al.Feulgen staining were performed in the tumor histological slides,and the technique of image cytometry was used to quantitate nuclear DNA ploidy and nuclear morphological parameters.The results combined with the data of clinical follow up were analyzed.Results:The mean DNA ploidy in grade Ⅰ～Ⅱ was mainly 2C,≥5C cells less than 10%;the 2C cells in grade Ⅲ～Ⅳ were lower than in grade Ⅰ～Ⅱ,≥5C cells were more than 20%.Among the four grades DNA ploidy were statistically different( P

Objective To investigate the feasibility of bedside temporary transvenous cardiac pacing with balloon tipped floating catheter under non X-ray fluoroscopy.Methods Twenty-four patients of bedside temporary transvenous cardiac pacing,11 cases underwent common electrode (common group),13cases underwent balloon tipped floating electrode (balloon tipped group).The operating time,pacing thresholds,pacing perception and complications were observed between two groups.Results All balloon tipped group placement success,8 cases in common group successfully.The operating time in balloon tipped group was significantly less than that in common group [(2.5 ± 1.5) min vs.(9.3 ±5.3) min],there was significant difference (P ＜ 0.05),but pacing thresholds and pacing perception between two groups had no significant difference (P ＞ 0.05).Common group occurred complications in 2 cases,respectively cardiac perforation and induced paroxysmal ventricular tachycardia,balloon tipped group occurred concurrent electrode wire dislocation in 2 cases.Conclusion Compared with the common electrode,balloon tipped floating electrode is simple,fast,high success rate and low risk of complications,suitable emergency physician bedside operating emergency temporary pacing.

Fracture Fixation ; Fracture Healing ; Humans ; Medicine, Chinese Traditional ; Radius Fractures ; therapy

80) via portal vein.After reperfusion 1 h,1 d,3d and 7 d respectively,the concentration of superoxide dismutase(SOD) and catalase(CAT) were tested,and the apoptosis of hepatocarcinoma also was observed using terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling(TUNEL) method.Results The results indicated that the SOD concentration in both hepatocarcinoma tissue and normal hepatic tissues decreased following I/R and perfusion with hyperoxic fluid liquid.The concentration of CAT increased following I/R in normal hepatic tissues.In hepatocarcinoma tissue,concentration of CAT decreased after reperfusion for 1 d and reached its lowest point.After perfusion with hyperoxic fluid,the concentration of SOD in both hepatocarcinoma tissue and normal hepatic tissues decreased more quickly following I/R and the low level was still found on 7 d after reperfusion.The concentration of CAT in tissues of both groups decreased and reached the lowest level at 1 h after reperfusion,but it was restored at 3 d reperfusion in normal hepatic tissues,and in hepatocarcinoma tissue was still at lower level until 7 d after reperfusion. After I/R,the apoptotic cells increased in normal hepatic and hepatic cancer tissues,and were most marked in tissues of hepatic carcinoma at 1 d and 3 d after perfusion with hyperoxic fluid.After I/R and perfusing with hyperoxic fluid,the changes of SOD and CAT and apoptosis in hepatocarcinoma tissue were more obvious than that in normal hepatic tissues(P

Objective To investigate the occurence of glomus tumor and investigate its diagnosis and treatment. Methods 13 cases of glomus tumors were analyzed retrospectively. There were 9 females and 4 males. All 13 cases were surgically treated under megaloscpe. Results All the cases were testified as glomua tumors by pathology. There was no recurrence during the follow up of 6 months to 1 year. All the patients were symptom free. Conclusion Glomus tumor has specific features on clinical presentation. Mierosurgieal excision under megaloscpe is fill elective method.

In order to fit new plot proposed by medical education reform,by integrating interrelated resources,the author introduced peer education into the whole process of nutrition propa-ganda and education to promote the development of the whole social health education.

In order to nurture the undergraduate majors of nutrition to fit society’s need,it is necessary to establish a new market-oriented talent training mode adaptable to both the market and society. This mode can also provide reference for the talent training of other emerging professions. Exploring the argument basis,theoretical design,practical development and research plan of this mode.

Objective To compare and analyze the difference of ?-tubulin and aurora-A expression in human cervical cancer cells (CasKi ) and immortalized human cervical squamous H8 cells with positive HPV16 E6E7.Methods Difference of ?-tubulin and aurora-A expression in CasKi and H8 cells was analyzed by showing the fluorescence intensity of ?-tubulin with indirect immunofluorescence.Expression level of aurora-A mRNA was detected by RT-PCR.Expression level of ?-tubulin and aurora-A in CasKi and H8 cells was semi-quantitatively analyzed by Western blot.Results The immunofluorescence signal of ?-tubulin was stronger in Caski cells than in H8 cells (57.78?3.13 vs 37.37?2.37,P

The T help cell 1(Th1) and Th2 cell classification have provided the framework for understanding CD4+ T cell biology and the interplay between innate and adaptive immunity for almost two decades. Recent studies have defined a previously unknown subset of the CD4+ T cell effectors, the Th17 lineage. The uncover on the differentiation of IL-17-producing effector T cells from naive T cell precursors provides insights into mechanisms by which signals from cells of the innate immune system guide alternative pathways of Th1, Th2 or Th17 development. Th17 lineage has an important role in autoimmune and inflammatory diseases. This promises to change our understanding on the immune regulation, immune pathogenesis and host defense. The identification, differentiation and immune function of the new subsets of Th cells, Th17 cells will be reviewed.

It has been a novel clinical way to repair damaged articular cartilage with autologous chondrocytes cultured in vitro using cartilage tissue engineering technique. There have been many articles about the techniques of chondrocytes culture in recent years. After summarizing and analyzing the involved articles,factors on chondrocytes cultured in vitro were reviewed,including dimension,stress,cell density and oxygen tension,etc. Chondrocytes cultured in three-dimensional with multi-factors in vitro can promote quality and quantity of cartilage in tissue engineering. But factors on chondrocytes culture are so complicated that further researches will be needed.