This paper was aimed to study the material basis for the efficacy of Miao medicine Bi-Ning spray in the treatment of allergic rhinitis,and to lay a foundation for improvement of its quality standard.Solidago and Centipeda minima were weighed according to the prescription and then soaked in water.Reflux extraction was conducted to the liquid.Petroleum ether,ethyl acetate,and n-butanol were used in the extraction,respectively.Each received composition was dried and made into sample solution for the experiment.A total of 60 healthy adult SD rats,with the ratio of half male and half female,were selected and randomly divided into 6 groups according to the weight with 10 rats in each group.There were the normal group,the model group,the petroleum ether (SYM) group,the ethyl acetate (YSYZ) group,the nbutyl alcohol (ZDC) group and the water group.The 20％ xylene olive oil solution was used in the model establishment of rats from different groups except the normal group.Intranasal administration of drug was given to different dosage group after the models were successfully established.Normal saline was given to the normal group and the model group.Serum of each experimental rat was collected at the end of the experiment.The contents of IgE,IL-4 and HT in serum were detected by ELISA.The pathological morphology of the nasal mucosa was analyzed.The results showed that compared with the normal group,the contents of IgE,IL-4 and HT in serum of the model group increased significantly (P ＜ 0.05).Compared to the model group,the contents of IgE,IL-4 and HT in serum of the water group decreased significantly (P ＜0.05).Contents of IgE,IL-4 and HT in other groups presented decreasing tendency.However,there was no statistical significance.The pathological morphology of the nasal mucosa results showed that compared with the normal group,the cell inflammatory response of the nasal mucosa tissues significantly increased in the model group (P ＜ 0.01).Compared with the model group,the cell inflammatory response of nasal mucosa tissues significantly decreased in the ZDC group and water group (P ＜ 0.05).There was no statistical significance in other groups.It was concluded that components in water part were the main material basis for efficacy of Bi-Ning spray in the treatment of allergic rhinitis.

This study was aimed to explore the effect of Periplaneta Americana against alcohol-induced liver injury in mice, in order to provide a theoretical basis for the industrial production of P. Americana. Kunming mice were randomly divided into six groups, which were the normal group, model group, positive group, high-, middle-, low-dose of P. Americana groups. Intragastric administration of Tiopronin Enteric-coated Tablets 100 mg·kg-1 was given to the positive group. Intragastric administrations of whole powder of medicine were given to the high-, middle-, low-dose groups with the dosage of 6.667, 3.333, 1.667 g·kg-1, respectively. The drugs were given daily for 10 con-secutive days. After 3h of the 10th day drug administration, intragastric administration of distilled water was given to the normal group, while 14mL·kg-1 of 56℃ Red Star Liquor was given to other groups. No food was given but water for 12h. Blood was collected from the orbit. The ALT, AST and GGT in blood serum of mice were measured. The liver was dissected and liver coefficient was calculated. Histopathological examination was given on liver tissues. The results showed that compared with the normal group, the level of ALT, AST in blood serum of the model group had obvious enhanced (P< 0.01), the level of GGT had obvious enhanced (P< 0.05). Compared with the model group, the level of GGT, AST of the high-, middle-dose group had obvious enhanced (P< 0.05), the level of ALT activity had obvious enhanced (P< 0.01). There were severe liver histopathological damages in mice of the high-, middle-, low-dose group. It was concluded that P. Americana had some side effects in the treatment of alcohol-induced liver injury.

OBJECTIVE:To establish HPLC fingerprint of Bining nasal spray.METHODS:HPLC method was performed.The determination was performed on Neptune C18 column with mobile phase consisted of acetonitrile-0.2 ％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 326 nm,and the column temperature was 25 ℃.The sample size was 10 μL.Using rutin as reference,HPLC chromatograms of 20 batches of samples were determined.Common peak identification and similarity evaluation were conducted by using Similarity Evaluation System for TCM Fingerprint (2012 edition).RESULTS:There were 40 common peaks in HPLC chromatograms of 20 batches of Bining nasal spray,with similarity ＞0.9.After validation,HPLC chromatograms of 20 batches of samples were in good agreement with the control fimgerprints of those.CONCLUSIONS:Established fingerprint can provide reference for identification and quality evaluation of Bining nasal spray.