This article analysis the present situation of less than 35 years of Sun Yat-sen university youth scientific and technological personnel,they declare、undertake to support basic research based science fundation related cases,discusses the science foundation for their assistance and training problems,put forward youth scientific talents to suit the science funds development requirements to promote their development proposals

OBJECTIVE:To compare the effects of2different treatment methods on the assaying of triptolide alcohol in tripterygium wilfordii cut crude drug.METHODS:After concentration,tripterygium wilfordii cut crude drug water decoction underwent column chromatographic separation after adsorption with neutral alumina(Method1)and neutral alumina column chromatographic separation after ethanol precipitation and extraction by chloroform(Method2),respectively.Triptolide alcohol was determined by HPLC and the assaying result underwent pairing and t tests.RESULTS:There was significant difference between the2methods with the content determined by Method1significantly higher than that by Method2(P

Alteration of H-and M-isozymfs of Lactic Dehydrogenase(LDH) were observed in the various tissues after exposing the rats to 50ppm and 250ppm of sulfur dioxide. These isozymes of the respective tissue were separated by Diethlaminoethyl(DEAE)-cellulose from tile tissue homogenates of brain, lung and muscle, presenting the activities by rate of reduction of nicot inamids-adenine-dinucleotide (NAD+). Pure LDH and the coenzyme NAD (NAD) were directly treated with sulfur dioxide in vitro order to find out the direct to sulfur dioxide on LDH and NAD+ and the results were as follow. 1. In the normal tissues, the H-isozyme activity was dominant in the brain and heart, and tole M-isozyme in the muscle. 2. In the lung tissue of normal rats, there was no different between tole activity of H-and M-type of LDH. 3 When rats inhale sulfur dioxide gas in concentration of 50ppm and 230ppm, it appeared that the H-type tend to be suppressed in aerobic tissues and the M-type in anaerobic tissues. 4. In the lung tissue exposed to sulfur dioxide, both the LDH activities were suppressed. 5. It seems that LDH and the coenzyme (NAD ) are not directly affected by exposing in sulfur dioxide gas.
Animals ; Brain ; Heart ; Isoenzymes ; Lung ; NAD ; Rats ; Sulfur Dioxide* ; Sulfur*

Objective To explore the effect of different treatments for breast cancer patients.Methods The clinical date of 24 patients with breast cancer were retrospectively analyzed.According to treatment method,24 patients were divided into 2 groups,control group of 12 patients received,and vinorelbine plus cisplatin.Experimental group of 12 patients received gemcitabine plus cisplatin.Both groups were treated for 6 weeks,follow-up in JanuaryFebruary,after treatment the short-term efficacy and toxicity were observed.Results During follow-up compared betwween two groups,after 6 weeks of treatment in patients with overall response rate ( complete response + partial response) are better than 1 week after treatment,the difference was statistically significant( P ＜0.05 ) ;between the two groups compared after treatment,experimental group patients overall efficiency as better than the control group,but had no statistically significant difference( P ＞ 0.05 ).In the control group,Ⅲ + Ⅳ leukopenia,nausea,vomiting and phlebitis rate were relatively high than experimental group,experimental group Ⅲ +Ⅳ thrombocytopenia was higher than control group,the differences were statistically significant( P ＜0.05 ).Conclusion Early application of gemcitabine plus cisplatin in patients with breast cancer could significantly improve,the survival and prognosis,improve patients quality of life,and safe,it's worthy of promoting in clinic.

Objective To investigate the diagnostic value of volume imaging for breast assessment (VIBRANT) dynamic enhanced MR for benign and malignant breast mass.Methods Ninety-eight patients with breast mass underwent both bilateral molybdenum target radiography and VIBRANT dynamic enhanced MR examination.The sensitivity,specificity,positive predictive value,negative predictive value and diagnosis accuracy between two methods were compared.Results There were 113 lesions including 65 lesions in 57 malignant cases and 48 lesions in 41 benign cases.The sensitivity,specificity,positive predictive value and negative predictive value of molybdenum target radiography were 67.69％ (44/65),68.75％ (33/48),74.58％ (44/59) and 61.11％ (33/54),respectively.The values of VIBRANT dynamic enhanced MR were 84.62％ ( 55/65 ),89.58％ (43/48),91.67％ (55/60) and 81.13％ (43/53),respectively.The difference of the diagnosis accuracy between two methods had statistical significance (P ＜0.05).Conclusion The diagnostic value of VIBRANT dynamic enhanced MR for benign and malignant breast mass is higher than molybdenum target radiography.

Objective To investigate the feasibility of bedside temporary transvenous cardiac pacing with balloon tipped floating catheter under non X-ray fluoroscopy.Methods Twenty-four patients of bedside temporary transvenous cardiac pacing,11 cases underwent common electrode (common group),13cases underwent balloon tipped floating electrode (balloon tipped group).The operating time,pacing thresholds,pacing perception and complications were observed between two groups.Results All balloon tipped group placement success,8 cases in common group successfully.The operating time in balloon tipped group was significantly less than that in common group [(2.5 ± 1.5) min vs.(9.3 ±5.3) min],there was significant difference (P ＜ 0.05),but pacing thresholds and pacing perception between two groups had no significant difference (P ＞ 0.05).Common group occurred complications in 2 cases,respectively cardiac perforation and induced paroxysmal ventricular tachycardia,balloon tipped group occurred concurrent electrode wire dislocation in 2 cases.Conclusion Compared with the common electrode,balloon tipped floating electrode is simple,fast,high success rate and low risk of complications,suitable emergency physician bedside operating emergency temporary pacing.

Lysosomal storage disorders (LSDs) are genetic defects caused by lysosomal hydrolase deficiencies. These deficiencies lead to substrate accumulation affecting cells, tissues and organs. Detecting abnormal compound excretion and deficient enzymes assist diagnosis of these disorders for treatment and prevention. This mini review summarizes clinical presentations and diagnostic workup of LSDs and updates the new development in the area.

Objective To investigate the effect of atorvastatin and fluvastatin in unstable angina patients after intervention on matrix metalloproteinase-9 (MMP-9) and interleukin-18 (IL-18).Methods One hundred and four patients with unstable angina were divided into atorvastatin group and fluvastatin grourp with 52 cases each by random digits table.Atorvastatin group was given atorvastatin 10 mg/d,and fluvastatin group was given fluvastatin 40 mg/d for 6 weeks on the basis of conventional treatment.The change of blood fat,MMP-9 and IL-18 was observed in two groups.Results There was no significant difference in MMP-9 and IL-18 on admission,1 d before operation and 1 d or 6 weeks after operation between atorvastatin group and fluvastatin group (P ＞ 0.05).The level of MMP-9,IL-18 1 d after operation in atorvastatin group and fluvastatin group was higher than that on admission,1 d before operation [(328.47 +49.15) μg/L vs.(237.10 + 57.32),(235.97 + 56.24) μ g/L,(218.75 + 38.00) ng/L vs.(120.42 + 25.50),(115.22 + 28.93)ng/L and (330.42 ± 50.04) μ g/L vs.(235.98 ± 60.36),(236.29 ± 53.02) μ g/L,(220.16 ± 37.12) ng/L vs.(119.43 ± 24.85),(117.14 ± 26.80) ng/L] (P ＜ 0.05).The level of MMP-9,IL-18 6 weeks after operation was decreased in atorvastatin group and fluvastatin group [(152.28 ± 43.20) μ g/L,(101.36 ± 23.67) ng/L and (150.30 ± 44.18) μ g/L,(100.45 ± 24.58) ng/L],which had significant difference compared with that on admission,1 d before and after operation(P ＜ 0.05).There was no significant difference in triacylglycerol (TG),total cholesterol (TC),high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) on admission,1 d before and after operation between atorvastatin group and fluvastatin group (P ＞ 0.05).There was significant difference in TG,TC,HDL-C,LDL-C 6 weeks after operation between atorvastatin group and fluvastatin group [(2.05 ± 1.00),(5.15 ±0.61),(1.84 ±0.50),(3.13 ±0.88) mmol/L and (1.87 ± 1.05),(5.52 ± 0.84),(2.82 ± 0.57),(2.61 ± 0.79) mmol/L] (P ＜ 0.05).There was no significant difference in TG,TC,HDL-C,LDL-C 1 d after operation compared with that on admission,1 d before operation (P ＞ 0.05).There was significant difference in TG,TC,HDL-C,LDL-C 6 weeks after operation compared with that on admission,1 d before and after operation in atorvastatin group and fluvastatin group (P ＜0.05).Conclusions In unstable angina patients after intervention,MMP-9,IL-18 can significantly increase.Atorvastatin or fluvastatin therapy can obviously reduce the MMP-9,IL-18 and lipid level,but fluvastatin shows better effect.

Objective To identify the value of medical journals by analyzing the papers covered in Medical F1000. Methods The papers published before November 2012 recommended by Medical F1000 were retrieved,the recommended ratios of the total number of papers published in journals each year were calculated and ranked.Results A total of 27960 papers were recommended by Medical F1000.Of these papers,801 were published in New England Journal of Medicine and ranked first.A great difference was found in the number of papers ranked according to their recommended ratios and the recommended number of papers.Conclusion Only a small number of papers are recommended by Medical F1000.However,they can help readers access to the valued medical information in the shortest time.The recommended ratio and the recommended number of papers are the important evidence for the assessment of journals.

The concept and characteristics of omnimedia were outlined, the effects of omnimedia on traditional in-formation service model, storage types of information resources, and personal service in medical library were analyzed, and a number of new information service models in the omnimedia era were put forward.