To evaluate the clinical value of NMP22 for the diagnosis of urothelial cancer. Urinary NMP22 was determined with enzyme linked immunoaorbent assay (ELISA) in 50 patients in whom 24 patients were suffering from cancer of urothelium, and 20 cases of cancer of other origins, and 6 cases of artificial bladder after total cystectomy for cancer. The median NMP22 value of urothelial cancer was 37 49U/ml, which was significantly higher than those of other patients (4 33U/ml, P

Objective:To explore the possibility of treatment of microencapsulated transgeneic cells on allotransplantation cells. Methods: The peritoned cavity of mice were injected with Alginate-chitosan microencapsules, which were prepared by drop generative technique and contained human fibroblastlike bone marrow stroma cells transgened by partial CEA gene. Results: The microencapsulated cells transplanted in mouse abdominal cavity could go on growing, proliferate and increase the immune reaction of T lymphocyte from mice spleen to ConA stimulation in 3 months. Conclusion :The study shows that Alginate-chitosan have good biocompatibility, strength and can be used as an immunoisola-tion tool in cell transplantation. The microencapsulated cell transplantation can help to broaden the cell source of allotransplantation and provide reliable proof for the study on treatment malignant tumor with microencapsulated transgeneic cell vaccine.

Objective To assess left ventricular systolic and diastolic function in patients with hypertrophic cardiomyopathy(HCM) with the aid of pulsed Doppler tissue imaging (DTI) to measure the four sides of mitral annular. Methods Thirty subjects with HCM and Twenty five age and sex matched controls were performed 2D and Doppler echocardiography . Two groups were studied with pulsed tissue Doppler imaging of the 4 sides of the mitral annulus (septal, lateral, inferior, anterior) in 4 and 2 chamber views. The velocities of systolic wave(Sa)、early diastolic filling wave (Ea)、late filling wave (Aa) and isovolumetric relaxation time were measured by DTI. Results Compared with normal control group, HCM group shows: (1) conventional data: LV wall thickness and mass indices were significantly greater. There were no significant difference in mitral flow Velocity. (2) DTI: There were more lower velocities of systolic and early diastolic filling wave. But isovolumetric relaxation time、mitra flow velocity / mitral annual early diastolic velocity ratio(E/Ea) increased. The velocities of systolic and early diastolic of septal site were the lowest among the 4 sides of the mitral annular and were the most closely with the average velocities of systolic and early diastolic of mitral annual. In addition, DTI may detect the pseudonormalization of mitral inflow. Conclusions DTI is more sensitive than conventional echocardiogram data and may be clinically used in exactly detecting left ventricular systolic and diastolic dysfunction in patients with HCM

To investigate the relationship between the level of plasma atrial natriuretic peptides(ANP) and doppler echocardiography parameters in patients with left ventricular diastolic dysfunction, the patients were divided into four groups according to echocardiography parameters and their plasma levels of ANP, which were determined simultaneously.The results showed that along with left ventricular diastolic function was impaired, the level of ANP increased.There were significant relationships between the level of ANP and LAD, E/A, LVDd, LVDs, EDV, ESV, IVRT, DT, S/D, LVEF, LVFS, except for Pva and Pva dur. The results of this study suggest that the level of plasma ANP is a reliable marker of left ventricular diastolic dysfunction.

objective To analyze the clinicopathological features and prognosis of antiglomerular basement membrane(GBM)disease,and evaluate the efficacy and safety of double filtration plasmapheresis(DFPP). Methods A total of 35 hospitalized patients diagnosed as anti-GBM disease in our department were enrolled in the study.All the patients were divided into 3 groups according to the manifestations at admission.Group Ⅰ∶24 patients with severe pulmonary hemorrhage or rapidly progressive glomerulonephritis(RPGN)received pulse methylprednisolone with or without DFPP,and then followed by prednisone and CTX.Group Ⅱ∶5 patients without severe pulmonary hemorrhage and RPGN received prednisone and CTX.Group Ⅲ∶5 ESRD patients and 1 normal renal function patient did not receive immunosuppression therapy.Anti-GBM antibody titer of pre-and post-DFPP in 4 patients was measured consecutively,and removal rate was calculated.Results The mean age of all the patients was(41.1±16.6)years.Sixteen patients(45.7%)presented Goodpasture's syndrome.Eighteen patients(51.4%)had anti-GBM glomerulonephritis alone,whereas one suffered solely from pulmonary hemorrhage.20%patients had positive P-ANCA serology.54.2%crescentic glomerulonephritis and 7 with other glomerulonephritis were revealed by kidney biopsy in 24 patients.Patients in Group Ⅰ showed more severe manifestation at admission:higher Scr level,higher titer of anit-GBM antibody,greater percentage of crescents.Within the follow-up period,7 patients died and kidneys of 50%patients survived.No patient died in Group Ⅱ and Ⅲ.The elder age,anemia,higher Scr(＞300 μmol/L),oliguria or anuria,emergency hemodialysis at admission,and more glomerular sclerosis were predictors of poor prognosis.The anti-GBM antibody was negative after 4 to 6 sessions of DFPP.and the mean removal rate was 55%.During total 94 DFPP sessions,there was no unacceptable morbidity. Conclusions Different therapy strategy is necessary for anti-GBM disease with different clinical manifestations.DFPP is an effective and safe clearance way of anti-GBM antibody.

Objective To investigate the mutations of pedocyte molecules in patients with late onset familial focal segmental glomerular sclerosis (FSGS). Methods Thirty-one pedigrees of late onset familial FSGS in Department of Nephrology, Shanghai Ruijin Hospital from Sep 1997 to Oct 2007 were enrolled in this study. The diagnosis standard of familial FSGS was as follows:(1) the age of presentation was more than 12 years old. (2) in one pedigree, two or more individuals were proven as FSGS by renal biopsy, or at least one was proven to be FSGS by renal biopsy, the others presented renal insufficiency or pmteinuria without precise causes. One hundred unrelated healthy people were screened as control group. Genomic DNA extracted from peripheral blood cells were amplified by PCR and then sequenced for mutations of NPHS2, ACTN4 and TRPC6. Results A novel missense heterozygotic mutation L316P of ACTN4 was identified inone pedigree. The mean onset age of the affected members of this pedigree was (38.7±7.4) years old and their kidney injury progress was slow. Proteinuria of the proband's brother was not improved by immunosuppressor. All 3 affected members of this family had such heterozygotic mutation. A novel missense heterozygotic mutation Q889K of TRPC6 was found in another pedigree. The mean onset age of the affected members in this pedigree was (38.0±4.2) years old. Three members presenting renal disease in this family all had such heterozygotic mutation but with different clinical manifestations. A quiescent mutation G467G of TRPC6 was also identified. Above variants were not found in healthy controls. No NPHS2 mutation was found to cause familial FSGS in these pedigrees. Conclusions A novel mutation L316P of ACTN4 and a new mutation Q889K of TRPC6 are identified in Chinese patients of late onset familial FSGS. No NPHS2 mutation is found to induce FSGS in these pedigrees.

OBJECTIVETo define the immune phenotype of colon cancer cells.

METHODSUsing a panel of 40 anti-human monoclonal antibodies (MoAbs), the cells of colon cancer HR8348 were analyzed with three-color flow cytometry after direct immunofluorescent staining.

RESULTSHR8348 cell line did not express CD2, CD3, CD4, CD5, CD7, CD8, TCR, CD10, CD11b, CD14, CD16, CD19, CD22, CD25, CD28, SmIg, CD33, CD35, CD36, CD41a, CD45, CD45RA, CD45RO, CD56, CD61, CD64, CD66b, CD69, CD71, CD117, CD122 and P-glycoprotein but expressed CD13, CD15, CD20, CD38, CD95 and HLA-DR.

CONCLUSIONThe results demonstrate that colon cancer cell line HR8348 shares some antigenic determinants with leucocyte lineage.

ATP-Binding Cassette, Sub-Family B, Member 1 ; analysis ; Antigens, CD ; analysis ; Cell Line, Tumor ; Colonic Neoplasms ; chemistry ; Humans

Objective To study the clinical and laboratory features of the mild and severe hand-foot-mouth diseases (HFMD) in Shenzhen in 2008.Methods 145 cases were observed in East-Lake Hospital and Shenzhen Children's Hospital. Of the 145 cases,124 mild eases and 21 severe cases were involved. All the clinical data and Laboratory findings were collected and summarized. After collection of the acute and convalescent consecutive stools and peripheral bloods from the patients with HFMD,EV71 genes were amplified from these samples by RT-PCR. Enterovirus 71 were cultured and isolated using Veto cell line and R&D cell line. Results The WBC counts and blood glucose levels of the severe cases were significantly elevated,but the ages of the severe ones significantly decreased compared with those of the mild cases( P < 0.05). EV71 genes could be detected by RT-PCR with 35% positive rate in mild cases and 67% in severe eases.The EV71 gene detection rate of the severe cases was significantly increased in contrast to that of the mild ones. The EV71 were isolated and cultured from the stools of 9 patients,one specimens from the dead's stool. Two severe cases died of neurngenic pulmonary edema and brain-stem encephalitis. Conclusions EV71 mainly contributes to HFMD and is responsible for death of some severe cases. High fever,less rash,elevated white blood cell counts and blood glucose concentrations as well as age less than 4 years old should be used for prediction of severe cases.