Objective To establish a gas chromatography method for the determination of hydrogen sulfide in waste gas. Methods Hydrogen sulfide was collected with 100 ml glass injector,then the sample was separated with the column of GDX-104, and it was detected by gas chromatography with the flame-photometric detector.Results The linear range was 0.000 5-0.85 mg/m~3, the linear equation was y=33.532 89+1.921 81x(r=0.999 86),RSD was 2.54%-3.89%,the lowest detected concentration was 0.000 5 mg/m~3.Conclusion This method is rapid,sensitive,accurate,stable and applicable to the determination of hydrogen sulfide in waste gas.

Study on protein expression and antigenicity detection of hepatitis C Virus envelope protein E2 in prokaryotic and eucaryotic expression systems.The gene that encoding.HCV E2 protein was cloned in pQE30 and pEF1/HisC.After the expression of E2 protein in E.Coli M15 and COS 7 cell,the expressed proteins were used to detect their antigenicity with ELISA and WB.The results showed that protein E2 was expressed in both prokaryotic and eucaryotic cells.Special reaction could be detected using the expressed proteins and sera from HCV infected people.The studied E2 gene could express the desired proteins in both prokaryotic and eucaryotic expression systems,and glycosylation of the E2 protein happened in COS 7 cell.

Objective To explore the correlation between Alzheimer's disease (AD), vascular dementia (VD) and carotid artery atherosclerosis. Methods This case-control study was conducted among 64 AD and 42 VD in-patients and out-patient admitted in Guangzhou Brain Hospital between July 2005 and July 2007, involving also 69 age- and gender-matched control subjects. Color Doppler ultrasound was performed to examine the artery shape, intima-media thickness (IMT), shape and size of the arteriosclerotic plaques, and diameter of the common carotid artery at 2 cm from the bifurcation and of the distal internal carotid artery 1.0-1.5 cm from the enlargement of the initial segment. Results Compared with those in the control group, the IMT, vascular diameter and arteriosclerotic plaques in the carotid artery were significantly increased in the dementia patients (P<0.05), but no obvious differences were found between the AD and VD patients. The degree of carotid artery atherosclerosis, represented by the number of plaques, was inversely correlated to the Mini-Mental State Examination scores (r=-0.389, P=0.000). Conclusion Carotid artery atherosclerosis is closely associated with the development of AD and VD, and the degree of atherosclerosis is positively correlated to the severity of dementia.

Objective To determine the risk factors related to tuberculosis infection among people living with HIV/AIDS and to develop strategies for preventing the co-infection.Methods A 1:2matched nested case-control study was carried out to analyze the influencing factors of tuberculosis among people living with HIV/AIDS.Results 1018 people living with HIV/AIDS were followed up for one year with a total number of 736.75 person-years,among them 62 tuberculosis cases were diagnosed.The incidence density of tuberculosis among people living with HIV/AIDS was 8.42 persons per 100 person-years.Factors as education level(OR=0.483),vaccination history of Bacille Calmette Guerin(OR=0.561),CD_4~+ count T-lymphocyte(OR=0.356),unemployment(OR=1.976),living alone(OR=2.646),and smoking(OR=2.215)were significantly related to the prevalence of tuberculosis among people living with HIV/AIDS.Conclusion High education level,with vaccination history of Bacille Calmette Guerin and high level of CD_4~+ T-lymphocyte count were protective factors while being unemployed,living alone,and smoking habit were risk factors related to the prevalence of tuberculosis among people living with HIV/AIDS.

OBJECTIVETo define the immune phenotype of colon cancer cells.

METHODSUsing a panel of 40 anti-human monoclonal antibodies (MoAbs), the cells of colon cancer HR8348 were analyzed with three-color flow cytometry after direct immunofluorescent staining.

RESULTSHR8348 cell line did not express CD2, CD3, CD4, CD5, CD7, CD8, TCR, CD10, CD11b, CD14, CD16, CD19, CD22, CD25, CD28, SmIg, CD33, CD35, CD36, CD41a, CD45, CD45RA, CD45RO, CD56, CD61, CD64, CD66b, CD69, CD71, CD117, CD122 and P-glycoprotein but expressed CD13, CD15, CD20, CD38, CD95 and HLA-DR.

CONCLUSIONThe results demonstrate that colon cancer cell line HR8348 shares some antigenic determinants with leucocyte lineage.

ATP-Binding Cassette, Sub-Family B, Member 1 ; analysis ; Antigens, CD ; analysis ; Cell Line, Tumor ; Colonic Neoplasms ; chemistry ; Humans

Pulmonary complications of ulcerative colitis are relatively uncommon and may present as a variety of disorders. Ulcerative colitis-related interstitial lung disease is extremely rare. There are a few case reports of nonspecific interstitial pneumonia in ulcerative colitis worldwide but none in Korea. We report a patient with ulcerative colitis related biopsy-proven nonspecific interstitial pneumonia, who responded to prednisolone (1 mg/kg) and mesalazine therapy
Colitis, Ulcerative* ; Humans ; Korea ; Lung Diseases, Interstitial* ; Mesalamine ; Prednisolone ; Ulcer*