Objective To investigate the clinical value of peri-interventional procedure serum bile acid(TBA)detection in patients with primary liver cancer. Methods The serum TBA was examined peri-operatively in 160 patients with primary liver cancer for testing the correlations between TBA, liver function, the degree of hepatocirrhosis, interventional therapy method and hepatic failure. Results The preoperative mean value of serum TBA incseased significantly in comparing with that of the control group (P

Objective To design and prepare recombinant mutant human high mobility group box 1 protein (HMGB1s) that can combine with HMGB1 receptors but cannot produce inflammatory effect, and accordingly lead to the creation of a new potential agent for anti-inflammatory therapy. Methods This experiment was based on successful clone and expression of human HMGB1.Six mutant HMGB1 cDNA were designed and constructed by one step inverse PCR. They were cloned into prokaryotic expressive vector pQE80L and followed with production of mutant HMGB1s and identification by Western blotting. Results Six mutant proteins were designed and constructed into prokaryotic expressive vector pQE80L. The recombinant HMGB1 proteins were obtained and identified by Western blotting. Conclusion Human HMGB1 mutants have been successfully constructed and the expression and characterization of intent proteins are identified. It will lay a foundation for further study on biological functions of HMGB1.

Objective To study the effects of ?-asarone on mitochondrial membrane potential(MMP) of cardiac myocytes with ischemia reperfusion injury. Methods The cardiac myocytes model of myocardial ischemia reperfusion injury (MIRI) was induced with sodium dithionite (Na2S2O4). The primary cultured cardiac myocytes of SD neonatal rats were randomized into six groups:normal group,model control group,high-dose ?-asarone (25 ?g/mL),middle-dose ?-asarone group(12.5 ?g/mL),low-dose ?-asarone group (6.25 ?g/mL) and medium group. Mitochondrial membrane potential(MMP) of myocardial cells were measured by flow cytometry. Results Compared with the normal group,MMP in the model control group was obviously decreased(P

Objective To study the protective effects of ?-asarone against the myocardial ischemia/ reperfusion injury (MI/RI) in cultured cardiac myocytes. Methods MI/RI model was set up with Na2S2O4, which make use of rat cardiac myocytes. The viability of cardiac myocytes (using MTT method), dehydrogenase (LDH, CK) activitices in the medium were measured. Results In MI/RI model, ?-asarone can obviously advance the viability of cardiac myocytes, and decrease the dehydrogenase (LDH, CK) activitices in the medium. Conclusion ?-asarone showed protective effects against the myocardial ischemia/reperfusion injury in cultured cardiac myocytes.

Objective To study the protective effects of ?-asarone on PC12 cells damage induced by Glutamate. Method The effects of ?-asarone on PC12 cells after Glutamate intoxication on morphology, extent of damage, livability, intracellular calcium concentration and apoptosis ratio were observed. Result Morphological changes, LDH leakage and intracellular calcium concentration increasing, and cell survival decreasing were observed in PC12 cells exposured to Glutamate. 7.5, 15, 30 ?g/mL?-asarone can increase cell survival, decrease LDH leakage. 15, 30 ?g/mL ?-asarone can reduce intracellular calcium concentration and apoptosis ratio. Conclusion ?-asarone prevents the toxicity of Glutamate, and it maybe attribute to its effect of anticalcium.

Objective To analyze the clinical and pathologieal characteristics of IgA nephmlogy secondary to primary sj(o)gren's syndrome(pSS).Method The clinical and pathological data of 8 patients with pSS and IgA in Changzheng Hospital from 2004 to 2009 were analyzed.Results The average age of seven female and one male was (44±8) years old.Five patients presented with edema and proteinuria.Eight patients presented with microhematuria.The average 24-hour proteinuria was (3±4) g.Two patients had hypertension.Serum creatinine levels of two patients were higher than normal level,the others'were normal.Light microscopy examination showed three patients were mild mesangial proliferation with Lee's classification grade Ⅰ;five patients had global sclerosis with Lee's classification grade Ⅱ,Ⅲ and Ⅳ.Positive IgA was mainly found under immunofluorescence microscopy.Electronic microscopy showed no electron-dense deposits.Conclusion IgA nephrology secondary to pSS is different from primary IgA nephrology under immunofluorescence microscopy.

Objective To observe the efficacy of ealcitonin and bisphosphonates on renal osteopathy of maintenance hemodialysis (MHD)patients. Methods Forty-three MHD patients were raindomly divided into two groups: A group and B group. All the patients were routinely received oral calcium carbonate 1.0 g tid and calcitriol 0.25 μg qd. Calcitonin (20U) hypodermic injection was given three times a week additionally during hemodialysis in A group. Patients in B group received bisphosphonates 70 mg once a week based on the therapy of A group. Serum levels of intact parathyroid hormone (iPTH), calcium, phosphorus, alkaline phosphatase (AKP), bone mass density (BMD) of lumbar spine and femoral neck, and the degree of bone ache (visual analogue scale, VAS) were assessed before the therapy and 3, 6 and 12 months after treatment. The adverse reactions were recorded during treatment. Results The levels of AKP and iPTH in both two groups decreased significantly after treatment. The above values of pre-treatment and 12 months after treatment were as follows: AKP(U/L)of A group 244.05±41.99 and 148.35±27.71,of B group 245.60±40.86 and 143.40±28.03;PTH(ng/L) of A group 697.5±119.7 and 267.4±45.9,of B group 708.2±120.3 and 277.6±41.9 (all P<0.05). While the levels of calcium and phosphorus did not change obviously during treatment (P>0.05). BMD was not improved at 3, 6 mouths and became better at 12 mouths after treatment. As compared to pre-treatment, BMD of lumbar spine(g/cm2) in A group was 1.062±0.223 vs 1.202±0.251 ,in B group 1.033±0.152 vs 1.189±0.225; BMD of femoral neck (g/cm2)in A group was 0.993±0.108 vs 1.067±0.095,in B group 0.947±0.083 vs 1.018 ±0.217 (all P<0.05). The scores of VAS also decreased significantly at 3, 6, 12 months after treatment(P<0.05). No severe adverse reaction was found during the treatment. Conclusions Utilization of calcitonin and combination with bisphosphonates during bemodialysis can effectively preserve the BMD and prevent bone loss in MHD patients and is well tolerated. No significant difference of therapeutic effect is observed between using ealcitonin or combination with bisphosphonates.

Background Posterior capsule opacification (PCO) is a common complication following cataract surgery.Establishing an objective morphological evaluation method of PCO is of important clinical significance for the prevention of PCO,but there are many problems in previous evaluating methods,and pentacam picture appears to be a useful method of assessing PCO.Recognizing the characters of different types of posterior capsule disorder and optimizing the evaluation details in tentacam picture are helpful for us to prevent and treat PCO.Objective This study was to investigate the characters of posterior capsule disorders with Pentacam tomograms.Methods Eightyfour eyes of 82 patients were included in Zhongshan Ophthalmic Center from January to October in 2011.Slit-lamp photography and retroilluminated photography were captured after full mydriasis,and then the Pentacam imaging was performed by using 50-scan acquisition protocol,and high-resolution Pentacam tomograms were reconstructed and analyzed by Image J software.The features of the posterior capsule disorders in Pentacam tomogram were assessed,and the mean pixel-intensity at the central 4-mm diameter and 3-mm diameter was calculated and compared.The percentage of eyes being interfered by the anterior capsule in the two analyed areas was calculated.This study was approved by Zhongshan Ophthalmic Center,and uritten informed consent was obtained from each patient prior to any examination.Results The posterior capsular membranes were completely clear under the blue background in 30 eyes without posterior capsulaopathy on the Pentacam images.In 54 eyes of 52 patients with PCO,different intensities of gray patches were exhibited on the blue background in the elschnig-pearl-type PCO,and white-color nontransparence patches were seen in the fibrosis-type PCO.The mean pixel-intensity value (inter-quartile range) was 37.07 U and 38.65 U at the central 4-mm area and 3-mm area respectively,without significant difference between them (P=0.606).Capsular block syndrome was found in 3 eyes of 3 patients,showing even gray appearance on the Pentacam images and mean pixel-intensity of 12.40,15.68 and 36.77 U at the central 3-mm area.The gray strip-like appearance on the blue background was seen in 1 eye with capsular shrinkage,and the mean pixel-intensity was 48.34 U at the central 3-mm area.The examination outcomes of 14.81％ (8/54) eyes were disturbed by the anterior capsule in the central 4-mm analysis mode,but the outcomes were unaffected in central 3-mm analysis mode.Conclusions Different types of posterior capsule disorders appear particular scenery in Pentacam tomograms.There is no difference of mean pixel-intensity between the central 4-mm analysis mode and the central 3-mm analysis mode.A central 3-mm analysis mode is more available to justify the penetrability of the posterior capsule.

Objective To evaluate the interventional management in emergency splanchnic bleeding and the application value.Methods 27 patients with emergency splanchnic bleeding underwent the interventional management in our hospital from May of 2003 to January of 2006 were reviewed.The sites and causes of the splanchnic hemorrhage were verified through selective DSA,using different methods and materials for percutaneous transarterial embolization.Results No bleedings recurred in all 27 patients within 18 months after the treatment.3 patients of advanced hepatic carcinoma died in 6 months because of non-splanchnic bleeding etiology.Conclusions Selective angiography is a veracious way to detect the location and cause of emergency splanchnic hemorrhage.The choice of different selective arterial embolizations and embolic materials can effectively and promptly cease the bleeding with rescuing the patients.

Objective To assess the safety and efficacy of tirofiban in stent-assisted coil embolization of intracranial aneurysms.Methods Totally 31 patients who were treated using stent-assisted coil embolization were analyzed retrospectively.All the patients were divided into aspirin combined clopidogrel group (double resistance group,n=18) and tirofiban group (n=13).Tirofiban was injected after the stent was semi-released or released completely.The patients were followed-up after operation to assess whether the patient had a new infarction,stent patency and aneurysm embolization effect.One day before operation and 3 days after operation,platelet count and activated thromboplastin time (APTT) were detected respectively.Score Glasgow (GOS) was used to evaluate the clinical outcome of patients and the operative complications were recorded.Results A total of 34 aneurysms were found in 31 patients,and 32 aneurysms were treated.Urinary tract hemorrhage was founded in 1 case and recurrent aneurysms were founded in 7 cases after embolization.None of the patients developed thrombocytopenia,retroperitoneal and gastrointestinal bleeding related to tirofiban administration.There were no postoperative cerebral infarction and thrombosis events.GOS of 27 cases were ≥4.Platelet count and APTT 1 day before and 3 days after operation had no statistically significance between two groups (both P＞0.05).Conclusion It is safe and effective for tirofiban in stent-assisted aneurysm embolization and can be used as an alternative to the dual resistance.