Objective:To explore the correlation among serum levels of brain natriuretic peptide (BNP) ,testosterone (T) , free three triiodothyronine (FT3) and heart function ,prognosis in male patients with chronic heart failure (CHF) .Meth‐ods:A total of 116 male CHF patients (CHF group) and 40 male patients with cardiovascular diseases and normal cardiac function (normal cardiac function group ) were enrolled .According to NYHA cardiac function class ,CHF group was divid‐ed into classⅡ group (n=41) ,class Ⅲ group (n=37) and class Ⅳ group (n=38);according to presence of cardiovascular events or not ,CHF group was divided into cardiovascular event group (n=36 ,event group) and non- cardiovascular event group (n=80 ,non- event group) .Serum levels of BNP ,T and FT3 were compared among above groups ,and the correla‐tions among those indexes and cardiac function were analyzed .Results:Compared with normal cardiac function group ,ser‐um BNP level significantly rose ,serum levels of T and FT significantly reduced in CHF group ( P<0.01 all) .Compared with classⅡ group ,serum BNP level significantly rose [(262.13 ± 125.08) ng/L vs .(674.65 ± 189.29) ng/L vs .(1072.91 ± 362.74) ng/L] in class Ⅲ group and class Ⅳ group ,and that of class Ⅳ group was significantly higher than that of classⅢ group;there were significant reductions in serum levels of T [(5.87 ± 1.12)μg/L vs .(4.56 ± 0.98)μg/L vs .(3.52 ± 0.74)μg/L] and FT3 [(4.70 ± 0.64) pmol/L vs .(3.68 ± 0.59) pmol/L vs .(2.18 ± 0.61) pmol/L] in class Ⅲ group and class Ⅳ group ,and those of class Ⅳ group were significantly lower than those of class Ⅲ group , P<0.05 or <0.01. Com‐pared with non - event group , there was significant rise in serum BNP level [(684.28 ± 192.76 ) ng/L vs . (992.63 ± 327.58) ng/L] ,and significant reductions in serum levels of T [(5.68 ± 1.04)μg/L vs .(3.35 ± 0.97)μg/L] and FT3 [(4.41 ± 0.78) pmol/L vs .(2.31 ± 0.62) pmol/L] in event group ,P<0.01 all .Linear correlation analysis indicated that cardiac function class was positively correlated with serum BNP level (r=0.314 ,P=0.023) ,and inversely correlated with serum T and FT3 levels(r= -0.272 ,-0.167 ,P=0.028 ,0.034) .Conclusion:Serum BNP ,T and FT3 levels are closely related with heart function in male CHF patients ,may provide important reference bases for diagnosis and evaluating prognosis .

Objective:To study the clinical efficacy of Carisolv in caries removal.Methods:20 primary teeth with middle and 20 with deep class V caries were randomly divided into two groups.Each group contained 10 teeth with middle caries and 10 with deep caries.Carisolv was used in test group and conventional rotary instrument in the control group. Efficacy of caries removal,treatment time, the degree of the pain and the current reaction of pulp between two groups were compared.Result:The Carisolv technique and the conventional method showed the same efficacy of caries removal.The treatment time in test group was longer than that in control group(P

Objective To investigate effects of hormone replacement therapy with conjugated equine estrogen (CEE) in different doses combined with medroxyprogesterone acetate ( MPA) on mammographic density among postmenopausal women and its clinical significance. Methods Ninety eligible postmenopausal women were randomized into three groups with varied treatment continuously for one year, Croup A receiving 0.3 mg CEE with 2 mg MPA and caltrate 600 mg and vitamin D 125 U daily; Group B receiving 0. 625 mg CEE combined with 2 mg MPA and caltrate 600 mg with vitamin D 125 U daily; and Group C receiving caltrate 600 mg and vitamin D 125 U daily. Mammographic density was analyzed using Wolfe and semi-quantitative methods for all of them and compared each other one year after treatment, as well as mammographic density before and after treatment in Groups A and B. Results One year after treatment, mammogrphic density reached the highest in postmenopausal woman of Group B and the lowest in those of Group C, with significant difference among the three groups (P ＜ 0. 01) and between Groups A and C (P ＜ 0. 01) and between Groups B and C ( P ＜ 0. 05 ). Mammogrphic density with semi-quantitative method increased significantly in Group A ( P ＜ 0. 05 ) and Group B ( P ＜ 0. 01 ), respectively, after treatment as compared with that before it. Change in mammografic density before and after treatment was greater in Group B than that in Group A, but not reaching statistically significant level (P ＞ 0. 05). Conclusions Hormone replacement therapy can cause the increase of mammographic density in postmenopausal women, possibly in a dose-dependent pattern, which suggest that HRT has side effect on breast tissue and the lowest effective dose of estrogen should be selected in HRT. Mammographic density can be used as an indicator to monitor side effect of HRT on breast.

Objective To investigate the value of CT guided interventional treatment in intracranial hematoma.Methods There were 108 cases with intracranial hematoma(30~100 ml,average of 50 ml) in our hospital from 2000~2003.Among them,58 cases were located in putamen,6 cases in subcortial,4 cases in thalamus,4 cases in cerebella,12 cases were traumatic eprdural hematoma and 12 cases were subdural hematoma.The table distance of the aspirated slice was the sum of table distance at the slice of the maximum hematoma and half slice thickness.It was considered successful for CT guided aspiration if the distance between the front of aspiration needle and the center ofthe biggest hematoma was not more than 1mm and it was regarded effective,when the patients' lives were saved and their life quality was improved.Results The success rate of CT guided aspiration was 99.8%.The effective rate of hypertensive intracerebralhemorrhage located in putamen,subcortial and thalamus was 94.3%.The effective rate of CT guided interventional treatment for cerebellar hypertensive intracerebral hemorrhage above 30ml was 0,the cure rate of traumatic epidural and subdural hematoma was 97.2%.Conclusion The effect of CT guided interventional treatment of intracranial hematoma is well except for cerebellar hematoma.

The effects of zinc on collagen synthesis and wound healing were observed in 124 rats. The animals were divided into three groups. ZD--zincdeficient, SP--pair fed with zinc supplemented and SF---ad lib withzinc supplemented, each receiving 0.07, 0.82 and 0.84 mg Zn per rat per day respectively. An 8 cm long surgical incision was made on one side of the back of each animal and 3 pieces of sponge were implanted subcutaneous^ on another side. The results showed that the rats receiving zinc supplement (SP , SF) had greater increase of body weight and higher concentration of serum zinc than the ZD rats before and after operation, Tke fibroblast counts in granular tissues of group ZD were significantly less than that of groups SP and SF from the 4th to 14th day after operation. The breaking strength of healing incision of groups S P and SF was greater than that of group ZD in the late stage of wound healing. The determination of sponge collagen indicated that groups SP and SF had stronger ability to synthesize collagen after being wounded than ZD animals. The results suggested that zinc might play an important role in wound healing, not only due to the increase of diet intake, but also due to increased fibroblast proliferation and collagen synthesis.

Aim To study the effect of tetramethylpyrazine(TMP) on binding of 125I-VEGF to VEGF receptor. Methods The mice sera were collected after peritoneal injection with big-dose TMP,low-dose TMP,protamine and NS. A reversed-phase high performance liquid chromatography(RP-HPLC) method was used to determine the TMP in mice serum. The culture medium of ECV304 was treated with the mice sera in different groups. Radioligand binding assay(RBA) of receptor and Scatchard pot were performed to observe the changes of the maximum binding capacity(B_ max) and dissociation constant(K_d).Results The sera of big-dose TMP inhibited 125I-VEGF binding to its receptor, K_d=343.30?36.64 pmol?L-1,B_ max=46.26?5.85 fmol/2?10~5 cells(P0.05),but B_ max decreased(P

Aim To explore the anti-angiogenic effect of tetramethylpyrazine(TMP) in the synovium of collagen-induced arthritis(CIA) in rats.Methods CIA rats were treated with different doses of TMP.The effects of treatment were monitored by arthritis index,footpad thickness,microvessels density as well as the expression of vascular endothelial growth factor(VEGF) protein and mRNA in the synovium.Results Compared with CIA models,100 mg?kg~(-1)?d~(-1) TMP remarkably reduce the arthritis index,footpad thickness,microvessels density,and the expression of VEGF protein and mRNA in the synovium (P

Objective To investigate the feasibility of low-c oncentration iso_osmolar contrast agent together with low tube voltage and iterative reconstruction algorithm in rabbit liver computed tonography (CT) perfusion imaging.Methods A total of 15 bealthy New Zealand rabbits were scanned twice of liver CT perfusion scans each with 24 hours interval.The first scan (routine group) was acquired at 100 kV and 100 mAs with ultravist (370 mg/ml),while the second (double low group) was acquired at 80 kV and 100 mAs with iodixanol (270 mg/ml) at 24 hours after the first scan.The obtained images were reconstructed with filtered back projection (FBP) and adaptive iterative dose reduction (AIDR-3D)algorithms in the controlled and experimental groups,respectively.The perfusion parameters including hepatic artery perfusion(HAP),portal vein perfasion(PVP),hepatic perfusion index(HPI),and total liver perfusion(TLP) and image quality as image quality score,average CT value of abdomen aorta,signalto-noise ratio(SNR),carrier-to-noise ratio(CNR),and figure of merit(FOM) were compared used pair ttest or Mann-Whitney U-test between the two groups wherever appropriate.The effective radiation dose and iodine intake were also recorded and compared.Results The image quality and perfusion parameters had no significantly different between the two groups except for FOM.The effective radiation dose and iodine intake were 38.79％ and 27.03％ lower in the double low group.Conclusions Low concentration iso _osmolar contrast agent (iodixanol,270 mg/ml) together with low tube voltage (80 kV) helps to reduce radiation dose and iodine intake without compromising perfusion parameters and image quality in liver CT perfusion imaging.

Objective: To explore the effects of different doses of Zn on the expression of metallo- thionein(MT) isoforms in hippocampus, olfactory bulb, diencephalon and cortex in stress rats. Method: The animal model of psychological stress was developed by restraint for 4w. Wistar rats were randomly assigned to 8 groups: control group, Zn-deficient group, pair-fed group, Zn -supplemented group and their corresponding stress groups. The expressions of MTs and their mRNAs were detected by Western blot and RT-PCR respectively. Results: In Zn-deficient group, plasma zinc content was decreased, while in Zn -supplemented group slightly increased. Compared with Zn-deficient group, the stress Zn -deficient group had higher expressions of MTs and MT-1 mRNA, MT-3 mRNA in hippocampus, olfactory bulb, diencephalon and cortex. Expressions of MTs in Zn-supplemented group were increased, and much more in corresponding stress group. In addition, the levels of plasma cortisol, IL-6 and IL-1 were increased clearly in Zn-deficient group and stress groups. Moreover, MTs’ productions were different in four brain domains,the highest in hippocampus and the lowest in diencephalon. Conclusion: Zn may affect the expressions of MTs and MT-1 mRNA, MT-3 mRNA in different regions of brain in stress rats. The higher production of MTs in hippocampus may be related to its role in body’s adaptation to stress. Cortisol, IL-6 and IL-1 may be involved in the modulation of expression of MTs.

Objective To observe the effects of psychological stress on the levels of Hcy and folate in rats. Method The Wistar rats were randomly assigned to three groups as follows:control,stress and stress with folate supplement. The animal model of psychological stress was developed by restraint. Results The levels of Hcy both in plasma and cerebrospinal fluid were increased and not only the level of folate in plasma but also in cortex,hippocampus,liver and mucous membrane epithelium of small intestine were decreased under restraint stress. Folate supplement could improve the depletion of folate and the increase of Hcy induced by restraint. Conclusion The depletion of folate may be one of the important factors of high Hcy level induced by psychological stress.