Objective The purpose of this report is to improve the recovery rate of diseases by integrating the advantages of mature treatment programs,with the method of diseases heterogeneity research.Methods Two programs (each program consist of effective group and invalid group) were took into investigation to study the characteristics of heterogeneity.If it showed that heterogeneous differences exist between the two effective groups,then we would treat the patients in different ways.Results The total effective rate of heterogeneous treatment was better than that of current optimal treatments.Conclusion Heterogeneous treatment could be applied to all diseases,either to TCM or to western medicine diseases.If the heterogeneous treatment can be popularized,it would improve the recovery rate of diseases surely.

Research of diseases' heterogeneity is a study method,which is based on the current ideas of single-program of clinical research,by means of searching the heterogeneous feature that corresponding to therapeutic schedule,optimizing and integrating the mature treatment programs,and aiming to improve diseases' recovery rate.Its characteristic is that it can integrate the advantages of current mature treatment programs,and make optimal effect.In this paper,we put forward the background,methods and ideas in the research of diseases' heterogeneity.

There are two treatments of A and B for disease X,if B replaced A,some patients that effective originally may become invalid.That is,the new drugs can increase the efficiency but may keep the effective patients out of the crowd at the same time.In this paper,we put forward the method of cake segmentation based on the principle of diseases' heterogeneity,optimize and make up the effective programs at present.The treatment based on diseases' heterogeneity will replace the best medicines currently,and format new treatments.

Syndrome differentiation of prescription is a more practical,simple and effective method which is distinguished from syndrome differentiation with eight principals,syndrome differentiation of viscera and syndrome differentiation of Wei Qi Ying Xue.However,after the ages of classical prescriptions,most of the current prescriptions,even ones from many experienced herb doctors' experiences,were seldom to summarize the syndrome corresponding to the prescription so precisely and to obtain reliable effect like the classical prescriptions.In this paper,by the research of diseases heterogeneity,we hope to excavate the syndrome which can correspond to the prescriptions of Chinese herbs and to set up a lot of prescriptions that can combine prescription and syndrome perfectly just like the classical ones in order to guide clinical treatment more conveniently and effectively.

Objective:To investigate the expression of CXCR4/CXCL12 and effect on the expression of CXCR4 by hypoxia in trophoblasts of human first trimester gestation.Methods:To detect the expression of CXCR4 and CXCL12 in placental tissue and primary cytotrophoblasts by immunohistochemistry and to assess the level of CXCR4mRNA under the condition of hypoxia in the trophoblasts of first trimester gestation by reas-time PCR.Results:The expression of CXCR4/CXCL12 were detected in the villous column,blood vessels and trophoblasts in the early pregnancy;CXCR4 mRNA was significantly higher through 12,24,48 and 72 h incubation periods in the hypoxic condition than that under normal oxygen condition respectively.Conclusion:Human first trimester trophoblasts produced CXCR4/CXCL12,which may play important biological role on the normal human pregnancy;Hypoxia could regulate the expression of CXCR4,which may contribute to the physiological pregnancy process.

Medical research contract is an important part of business between medical research organizations.It is the official document identifying the right and obligations of respective medical research organizations during research activities.It is the legal basis,and is also on the basis of research expenditure.Therefore,it is very important to improve ability of the contract writing in research management.Composing a sound medical research contract has significant importance in research activities.This paper explored the remarkable issues when composing a medical research contract from the respective of management of funding,including how to make the contract more fair,rational,rigorous and legal.

ObjectiveTo determine whether the β2-agonist, clenbuterol, can improve recovery of locomotor function following spinal cord injury.MethodsThe spinal cords of rats were contused with a weight-drop apparatus similar to the NYU impactor at the lever of T10. Locomotor function was determined with the Basso, Beattie, Bresnahan (BBB) Scale. Following 6 weeks of behavioral evaluation, the spinal cords were received histological examination.ResultsClenbuterol improved the recovery of locomotor function and increased the amount of spared spinal cord tissue.ConclusionClenbuterol caused the enhancement of recovery of locomotor function and spared spinal cord tissue following contusion.

ObjectiveTo explored the nursing about the patients received Onyx embolization. Methods14 patient with cerebral arteriovenous malformation received Onyx embolization were analyzed retrospectively.ResultsThere was no long-term complication occurred, and all the patients left hospital 3～6 d after operation. ConclusionIntensive nursing on lowered blood-pressure control was the most important process to keep embolization effect successfully.

Objective To retrospectively analyze the influence factors during autologous peripheral blood stem cell (PBSC) mobilization and collection process in the patients with non-Hodgkin′s lymphoma (NHL).Methods Forty-eight patients with NHL in our hospital from January 2014 to August 2016 underwent PBPC mobilization and collection.The factors of age,sex,disease stage,bone marrow involvement at diagnosis,chemotherapy frequency,relapse and so on were analyzed retrospectively.Results PBSC mobilization and collection was successful in 37 cases (77.10%).The success rate was affected by the time from diagnosis to mobilization (P=0.038),chemotherapy courses (P=0.016),prior chemotherapy including fludarabine (P=0.049),relapse(P=0.033),and the levels of white blood cell (P=0.014) and platelet (P<0.01) before stem cells collection.Conclusion For the patients planning to receive autologous peripheral blood stem cell transplantation,many times of prior chemotherapy are unfavorable,mobilization and collection should be taken as early as possible,chemotherapeutic agents which possibly injure stem cells should be avoided,it is needed to simultaneously pay attention to peripheral blood WBC and platelet level for determining the collection timing.

The enantiomers separation of thirteen drugs collected in Ch.P2010 was performed on chiral stationary phase of cellulose ramification (chiralpak OD and chiralpak OJ) by high performance liquid chromatographic (HPLC) methods,which included ibuprofen (Cl),ketoprofen (C2),nitrendipine (C3),nimodipine (C4),felodipine (C5),omeprazole (C6),praziquantel (C7),propranolol hydrochloride (C8),atenolol (C9),sulpiride (C10),clenbuterol hydrochloride (C11),verapamil hydrochloride (C12),and chlorphenamine maleate (C13).The mobile phase consisted of isopropanol and n-hexane.The detection wavelength was set at 254 nm and the flow rate was 0.7 mL/min.The enantiomers separation of these thirteen racemates on chiralpak OD column and chiralpak OJ column was studied,while the effects of proportion of organic additives,alcohol displacer and temperature on the separation were studied.And the mechanism of some of racemates was discussed.The results indicated that thirteen chiral drugs could be separated on chiral stationary phase of cellulose ramification in normal phase chromatographic system.The chromatographic retention and resolution of enantiomers could be adjusted by factors including column temperature and the concentration of alcohol displacer and organic alkaline modifier in mobile phase.It was shown that the resolution was improved with reducing concentration of alcohol displacer.When concentration of organic alkaline modifier was 0.2％ (v/v),the resolution and the peak shape were fairly good.Most racemates mentioned above had better resolution at column temperature of 25 ℃.When racemates were separated,the temperature should be kept so as to obtain stable separation results.