A patient with sparganosis mansoni was treated with praziquantel and cured with little side effect.

A patient with eosinophilic syndrome due to Paragonimus infection wag treated with praziquantel and cured.

Objective To compare the pass rates of two primary hearing screening times and to determine the appropriate screening time.Methods The newborns born in 2004 and 2005 at our hospital were divided into two groups: group A and group B.Group A received hearing screening with distortion product otoacoustic emission(DPOAE) on the 3rd day after birth,while group B screened in one month after birth.The newborns who failed the initial screening were rescreened one month later.The babies with positive screening findings were referred to full auditory assessments.Results Group A(n=2 305) had pass rate 81.9% at the initial screening and 85.2% at rescreening.Group B(n=1 348) had pass rate 93.9%(?2=103.99,P

Objective To explore the action mechanism of basic fibroblast growth factor (bFGF) on vascular smooth muscle cells (VSMCs) in spontaneously hypertensive rats (SHR).Methods Ts were obtained from SHR and SD rats.The aortic VSMCs were cultured in vitro by tissue explant method.VSMCs were treated with different concentration of exogenous bFGF (0 ng/ml,20 ng/ml,40 ng/ml,60 ng/ml,80 ng/ml,100 ng/ml) for 48 h,then cell proliferation was detected by 3 (4,5 dimethylthiazol)2,5 diphenyltetrazolium bromide (MTT) colorimetric assay.VSMCs from SHR in control group were treated with bFGF (100 ng/ml) for 48h.VSMCs from SHR in treatment group were treated with bFGF (100 ng/ml) plus Proteinkinase C(PKC) inhibitor (staurosporine) for 48 h.Results After treatment with different concentration (0 ng/ml,20 ng/ml,40 ng/ml,60 ng/ml,80 ng/ml,100 ng/ml) of bFGF for 48 h,the values measured by MTT colorimetric method were 0.402 ± 0.103,0.605 ±0.090,0.696 ± 0.131,0.812 ± 0.080,0.901 ± 0.065,1.056±0.078 respectively in aortic VSMCs from SD rats,and 0.404±0.065,0.507±0.078,0.608±0.057,0.704 ± 0.107,0.812 ± 0.097,0.908 ± 0.032 respectively in aortic VSMCs from SHR.Compared with control group,the values measured by MTT colorimetric method were decreased in treatment group (P＜0.05).The proliferative effect of bFGF in aortic VSMCs from SHR was attenuated after administration of PKC inhibitor staurosporine.Conclusions Exogenous bFGF administration promotes VSMCs proliferation in SHR and SD rats in a concentration-dependent manner.PKC plays an important role in the signal transduction mechanism in VSMCs proliferation by exogenous bFGF.

OBJECTIVEThe purpose of this study was to investigate the relationship between the results of flowcytometry analyses of different clinical stage, location, pathologic grade and cell origin of oral and maxillofacial non-Hodgkin's lymphoma (NHL), and the diagnostic value of flowcytometry analysis in lymphoma.

METHODThis study analyzed 50 oral and maxillofacial NHL cases and 10 reactive lymph nodes (formalin fixed and paraffin embedded) by flowcytometry (FCM).

RESULTSReactive lymph nodes were all diploid. The diploid rate of NHL was 54%, and aneuploidy rate was 46%. There was statistically significant difference between reactive lymph nodes and NHL in the DNA ploidy status and cell cycle data (SPF, CV, S + G2/M, DI). The S phase fraction (SPF) and S + G2/M had close relationship with the grade of NHL. SPF value and DNA ploidy status had no obvious relationship with the prognosis.

CONCLUSIONThe results suggested that the FCM had diagnostic value in NHL, especially when the morphological diagnosis was difficult. Although the cell cycle data had no prognostic value, SPF and SPF + G2/M can show the proliferative status of NHL, which can help clinical doctor select therapeutic method.

Cell Cycle ; DNA, Neoplasm ; analysis ; Facial Neoplasms ; diagnosis ; genetics ; pathology ; Flow Cytometry ; Humans ; Lymphoma, Non-Hodgkin ; diagnosis ; genetics ; pathology ; Maxillary Neoplasms ; diagnosis ; genetics ; pathology ; Mouth Neoplasms ; diagnosis ; genetics ; pathology ; Ploidies ; Prognosis

Objective To explore the effect of praziquantel on schistosomal ovum granuloma in the lung of mice.Methods Forty-eight mice were divided into 4 groups.Group A:first,the mice were injected with schistosomal ova hypodermicly in abdomen and 10 days later,injected with schistosomal ova intravenously in the cauda;Group B:in addition to the injection of schistosomal ova as the same of Group A,the mice were administered with praziquantel [300 mg/(kg?d)] for 3 days from the last day of the intravenous injection of the ova;Group C:in addition to the injection of schistosomal ova as the same of Group A,the mice were administered with praziquantel(75 mg/kg,B.i.d.)for 5 days weekly until the mice were sacrificed;Group D:the same as Group C but praziquantel was given to the mice from the 29th after the intravenous injection of the ova.Three mice of each group were sacrificed on the 7th,14th,28th,56th day after the intravenous injection of the ova and the lung tissues were fixed with formalin and the slices were HE stained.Fifteen-thirty pieces of schistosomal ovum granuloma were examined and their areas were measured and the mean areas of each group were calculated and compared.Results On the 7th,14th and 28th days after the intravenous injection of the ova,the mean area of schistosomal ovum granuloma in Group C was significantly less than that in Group A,and there was a significant difference between the two groups,P 0.05.On the 56th day,the mean areas of schistosomal ovum granuloma in Group B,C,D were significantly less than that in Group A,all P

Objective To evaluate the molluscicidal effect of 2 kinds of shamingdan in lab. Methods Under constant temperature (25?1) ℃, the studies were carried out by using different concentrations(dosages) of molluscicide, shamingdan, in various methods of immersing, spraying and dusting power, and the molluscicidal effects were compared with 50% wettable powder of niclosamide ethanolamine salt(WPN). Results The 48-hour's LC_ 50(s) of 4% shamingdan granular formulation and 50% shamingdan soluble powder were 0.1752 mg/L and 0.2713 mg/L, respectively, which were similar to that of 50% WPN. In spraying and dusting powder experiment, the 7-day's LC_ 50(s) were 3.0418, 0.1623 g/m~2 and 2.8752, 1.1101 g/m~2, respectively, and the 50% shamingdan soluble powder was better than 4% shamingdan granular formulation and 50% WPN. Conclusions The molluscicidal effect of 2 kinds of shamingdan using immersing is better than that using spraying and dusting. The molluscicidal effect of the 50% shamingdan soluble powder is remarkably better than that of the 4% shamingdan granular formulation and 50% WPN.

Objective To evaluate the effectiveness of the current measure of Oncomelania hupensis control in Jiangsu Province. Methods The snail control was carried out with molluscicides in the high transmission areas every year. Some snail habitat areas were modified. The snail areas within three years were re-treated with molluscicides in the maintenance phase. The snail survey was carried out every spring, and the data were analysed with SAS software. Results From 1995 to 2001, 14519.17 hm 2 of snail habitats were molluscicided, 2768.57 hm 2 were modified, and 8803.64 hm 2 were re-treated with molluscicides in the maintenance phase. The coverage rates of snail control areas dropped by 19.14% every year. The snail areas increased by 6.25% every year from 1995 to 2002. In which, the areas of infected snails increased by 18.52% every year. The correlation analysis showed that the areas of infected snails increased with the increasing of the total snail areas. At the same time, the areas of infected snails increased with the fall of the coverage rates of snail control areas. The analysis of the snail distribution showed that the main problem was poor snail control. Conclusion In recent years, the rise of area of snail habitats is serious in Jiangsu Province. The present measures of the snail control have not effectively stopped the spreading and increasing of snails. The research on the new molluscicides, the new methods of snail control and the better policies are very important.

Objective To understand the distribution and fluctuation tendency of infected Oncomelania snails in marshland areas of Jiangsu Province in order to provide the scientific basis for acute schistosomiasis control. Methods In each county(city, district) 5-10 marshland areas with snails were sampled randomly, and of which 5-10 sites were selected. Fifty to one hundred snails were collected in each site and dissected by professionals to determine the natural infection rate. At the same time, the mollusiciding actions were carried out in the schistosome-positive snail sites and beaches. Results In 5 years, a total of 467 beaches have been checked and 102 positive beaches were find out and the snails detected. The positive rate of beaches was 21.84%(102/467). A total of 169200 snails were dissected , and of which 433 were positive snails. The natural infection rate was 0.2559%(433/169200). The infested areas and the number of positive snails in 2002 were 2.71 and 18.33 times of those in 1998 in Jiangsu Province. Conclusion In the continuous 5 years observation, the tendency of the population of positive snails and the infested areas were significantly growing, and there is a apotential threat for the acute infections. So, the keys of the following work should be controlling snails seriously in the marshlands and the rivers in connection with the Yangtze River, increasing the mollusiciding cover rate and improving the quality in the positive beaches, and surveying and treating the cattle time by time.