Objective To compare the effects of endoscopic probe and self-made balloon device in dilation of the cardia for patients with cardia achalasia.Methods From January 1998 to December 2007,45 patients with cardia achalasia received gastroscopy-assisted dilation at our hospital;22 of them were treated by self-made balloon device and 23 were by endoscopic probe.The efficacies of the two methods were compared.Results In the probe group,each patients received 1 to 9 times of dilation(mean,3.6),while in the balloon group it was 1 to 6 times(mean,2.3).None of the two groups had intra-and post-operative complications.The cost for the first treatment was(1542.57?281.30) yuan in probe group,and(861.91?176.48) yuan in the balloon group(t=9.671,P=0.000).The efficacy of the two groups was similar in 3 months after the treatment [the rates of the cured,improved,and failed were 61%(14),35%(8),and 4%(1) in the probe group,and 68%(15),23%(5),and 9%(2) in the balloon group,Z=-0.351,P=0.726],but significantly different in 6 months [22%(5),13%(3),and 65%(15) vs 45%(10),32%(7),and 23%(5),Z=2.564,P=0.010].Conclusions Both endoscopic probe and self-made balloon device are effective in dilatation of the cardia for cardia achalasia.Self-made balloon device dilatation is exceptionally suitable for local hospitals because of its low cost and simple techniques.

Objective To investigate the value of pleural effusion and C-reactive protein in severity evalua-tion and prognosis of acute pancreatitis. Methods Clinical data of 108 patients with acute pancreatitis were ana-lyzed retrospectively. AP classification was conducted on the basis of intensified CT examination and the clinical in-dexes including blood calculus, blood lipid, blood glucose and blood gas were measured. The criteria for diagnosis and severity evaluation of acute pancreatitis were based on results of chest X-ray, CT examination and CRP. The correla-tion between pleural effusion, CRP and the severity, etiology, prognosis of acute pancreaitis were analyzed. Results Of the 108 patients, mild acute pancreatitis (MAP) was found in 57 patients, and severe acute pancreatitis (SAP) in 51. Among SAP, 32 patients (62.75% ) developed pleural effusion, 38 patients (74.51% ) whose CRP > 20.00 mg/L,and 27 patients (52. 94%) had both pleural effusion and CRP > 20. 00 mg/L. There were 13 (22.81% ), 14 (24.56%) and 8 (14.04%) respectively among MAP. The difference between the two groups was sig-nificant(P<0.01). Among acute biliarv pancreatitis,9 patients (33.33%) developed pleural effusion and 15 (55.65%) whose CRP>20 mg/L and 9 (33.33%) had beth pleural effusion and CRP>20.00 mg/L; Among acholic AP,25 (65.79%) developed pleural effusion and 20 (52.63% ) whose CRP > 20.00 mg/L and 23 (60.53%) had beth pleural effusion and CRP>20.00 mg/L. Case fatality was also significantly different between group with pleural effusion,CRP >20.00 mg/L,both pleural effusion and CRP>20.00 mg/L,and group with non-pleural effusion,CRP≤20.00mg/L,non-pleural effusion or CRP≤20.00mg/L respectively(P<0.01). Conclusion Either pleural effusion or CRP can be used as independent prognostic parameters for severe acute pancreatitis,and the combined use of these two parameters is the most reliable.

Objective To investigate the effect of artemisinin on the proliferation of human hepatoma cell line HepG‐2 .Methods The inhibition effect of cell proliferation in human hepatocelluar carcinoma cell line HepG2 of artemisinin was detected by MTT test ,and the cell cycle and apoptosis were detected by Flow cytometry .Results Artemisinin at 80 umol/L could effectively inhibi‐ted the proliferation of HepG‐2 cell in a dose‐and time‐dependent manner;the drugs could block cells at G0/S phase ,and induct the HepG‐2 cell apoptosis .Conclusion Artemisinin could effectively inhibit the proliferation of HepG‐2 cell .

Objective To devolope a method for extracting DNA from dried blood spots (DBS)and optimizing the operating procedure,which could be applied to clinical gene diagnosis of thalassemia.And the cross contamination of DBS punching and the storage stability of DBS were studied.Methods A total of 1 50 blood specimens were collected,and DBS were prepared.Circles (3 mm in diameter)were punched in the DBS,and eluted with lysis buffer.The eluting method and operating procedure were opti-mized.Genomic DNA extracted from the elution solution by magnetic beads,and were performed thalassemia gene test.Finally jud-ging whether the results of DBS and whole blood were consistent.Two methods of thalassemia gene test were used in DBS and the compatibility of DBS processing method was verified.Judging whether there was cross contamination of DBS punching by the thalassemia gene test results of blank hole which were punched in the blank filter paper between thalassemia positive DBS.The DBS storage stability in thalassemia gene test was verified by detecting the DBS which were dry stored at room temperature for 6 and 9 months.Results 5 circles (3 mm in diameter)DBS were vibrating eluted at 55 ℃ for 1 hour,the DNA concentration extracted from the elution solution was 10-20 ng/μL,which was dissolved in 50 μL solution,and the DNA quality was good.The thalassemia gene test results of DBS and whole blood were the same,and the DBS results of two thalassemia gene test methods were the same too. The cross contamination of DBS punching was not detected in thalassemia gene test.The DBS which were dry stored at room tem-perature for 6 and 9 months could be stably performed thalassemia gene test.Conclusion DBS could be used to perform thalassemia gene test,which is accurate,convenient and stable.It is an ideal way for specimen referral of thalassemia gene test.

OBJECTIVE: To investigate a new way to yield plenty of high purity olfactory ensheathing cells (OECs) and its biocompatibility with appropriate scaffolds. METHODS: OECs were prepared from neonatal Wister rats and co-cultured with poly [LA-co-(Glc-alt-Lys)] (PLGL). Its contact angle, adherent rate, and activity rate were tested. RESULTS: The contact angle of poly (D, L-lactic acid) (PDLLA) (84.5 degree+/-1.5 degree) was significantly higher than that of PLGL (52.6 degree+/-0.8 degree), the adherent rate of PLGL (80%) was significantly higher than that of the PDLLA (57%), and the activity rate of PLGL (88%) was much higher than that of the PDLLA (76%). CONCLUSION PLGL possesses better hydrophilicity and biocompatibility than PDLLA, and it can provide a better cell growth circumstance which is helpful for the effective treatment of nerve injury.
Animals ; Animals, Newborn ; Biocompatible Materials ; Cells, Cultured ; Lactic Acid ; chemical synthesis ; pharmacology ; Nerve Regeneration ; Olfactory Bulb ; cytology ; Polyglycolic Acid ; chemical synthesis ; pharmacology ; Polylactic Acid-Polyglycolic Acid Copolymer ; Rats ; Rats, Wistar ; Spinal Cord Injuries ; physiopathology ; Tissue Engineering ; methods ; Tissue Scaffolds ; chemistry

To establish an experimental model of osteoblasts to easily cause calcification of bone matrix in vitro, we took cranium of a newborn rabbit out under an aseptic condition, removed the connective tissue of the bony suture, and cut the cranium freely into the fragments of not more than 1 mm2. The we isolated and cultured the osteoblasts using tissue explant method. We observed growth status of primary osteoblasts and subcultured osteoblasts using inverted microscope. Then we conducted enzyme staining and alizarin red staining for the third generation of osteoblasts to detect the alkaline phosphatase (ALP) expression and calcified nodules. The result showed that there were calcified nodules or calcification formed after the primary osteoblasts climbing out from the bone for 1 week, and each generation of osteoblasts had the similar calcification with the primary osteoblasts, and there was an increase in calcified nodules after the continuous culture. There was a strong expression of ALP in the plasma membrane of osteoblasts. The calcified nodules were red with alizarin red staining. It is well concluded that osteoblasts isolated with this method easily cause calcification, and can be used as a new experimental model.
Alkaline Phosphatase ; metabolism ; Animals ; Animals, Newborn ; Cell Separation ; methods ; Osteoblasts ; cytology ; Primary Cell Culture ; methods ; Rabbits ; Skull ; cytology

OBJECTIVEDiagnosis and prenatal diagnosis to a family of hemoglobin variant with α-thalassemia.

METHODSWhole blood cell analysis, hemoglobin analysis by capillary zone electrophoresis (CZE), Gap-PCR, polymerase chain reaction-reverse dot blot (PCR-RDB) assay and DNA sequencing.

RESULTSHb Zurich Albisrieden with α°-thalassemia lead to severe anemia. The genotype of fetus is also Hb Zurich Albisrieden with α°-thalassemia.

CONCLUSIONAbnormal hemoglobin with α-thalassemia may lead to severe anemia, Prenatal diagnosis of thalassemia has the vital significance for eugenic birth.

Adult ; Base Sequence ; Child, Preschool ; Female ; Fetal Diseases ; blood ; diagnosis ; genetics ; Hemoglobins, Abnormal ; genetics ; metabolism ; Humans ; Male ; Molecular Sequence Data ; Pregnancy ; Prenatal Diagnosis ; Young Adult ; alpha-Thalassemia ; blood ; diagnosis ; embryology ; genetics