Objective: To provide standardized alternative answers with the feature of equal intervals in the quality of life assessment.Methods:We collected 131 questionnaires (123 for psychological test and 8 for quality of life assessment). The SPSS 10.0 was used to analyze the frequency distribution of the alternative answers used in these questionnaires.Results:The most commonly used design for alternative answers is five levels, which comprises 31.68% of the total designs, followed by four levels (21.99%) and three levels (13.35%). The words used in the alternative answer show great diversities.Conclusion:We recommend that the alternative answers be designed as five levels, it is better to apply commonly used words in the alternative answer and have a feature of equal interval.

Objective We categorized chronic constipation according to colonic transit time (CTT) and transit index (TI), and investigated the abnormalities of anorectal motility and sensory function in patients in different categories. Methods Each subject ingested 20 radiopaque markers, and abdominal X rays were obtained at 72 h. Chronic constipation was categorized according to CTT and TI. Anorectal manometry was carried out in all subjects to investigate the abnormalities of anorectal motility and sensory function in patients in different categories. Results Chronic constipation was divided into four types: normal transit constipation (NTC), slow transit constipation (STC), outlet obstructive constipation (OOC), and mixed type constipation (MC). Patients with constipation displayed significantly lower anorectal resting pressure, squeezing pressure and larger rectal maximum tolerate volume than controls ( P

Objective:To investigate the status of oral hygiene behavior of the people of 1 8 yeas old and above in Guizhou prov-ince.Methods:A total of 9 280 adults were included by multi-staged sampling from 1 2 counties in Guizhou province of China.The double entry data were processed by Epiinfo 3.5.1 and statistically analysed by SPSS 1 9.0.Results:The survey of 9 280 subjects were permanent residents from 201 0 -201 1 .1 5.9% of the total subjects experienced dental visit,those from city and contrside were 23.0%,1 2.5%;male and female were 1 4.4%,1 7.1 %,respectively.5.6% of the them experienced tooth scaling,those from city and countryside were 9.9% and 3.4%,male and female were 4.9%,6.2% respectively.The subjects with 1 time and 2 time tooth brushing a day were 58.1 % and 28.6%,respectively.6% of the subjects did not brush teeth,mainly were those over 60 years (21 .3%),those of city residents was significantly lower than countryside(7.0% vs 4.0%).Conclusion:The oral heal behavior of Guizhou residents is poor,the education of oral health should be strengthened.

Objective To investigate the relationship between LUTS and degree,type of prostate histological inflammation in patients with benign prostatic hyperplasia (BPH).Methods From October 2013 to June 2016, 150 patients with BPH(62 -82 years old)were treated with selective TURP.After operation,the pathology examina-tion was practised.According to the international prostatitis histological classification standard,the patients were divid-ed into group A(without inflammation,18 cases),group B(inflammation around gland,45 cases),group C(gland inflammation,33 cases)and group D(mixed inflammation group,54 cases).There were no statistically significant differences among the 4 groups in age,course of disease,number of urinary retention and prostate volume(all P >0.05).The IPSS score before and 2 months after operation and the correlation between the IPSS and degree,type of histological inflammation were observed.Results In group A and C,the preoperative IPSS score and IPSS score at 2 months after surgery were (17.20 ±11.14)points,(8.45 ±5.14)points and (18.05 ±13.11)points,(9.85 ± 5.25)points respectively,there were no statistically significant differences between the two groups(t =0.485,1.294, all P >0.05).There were no statistically significant differences between group B[(22.55 ±11.85)points,(13.24 ± 6.46)points]and group D[(21.45 ±10.35)points,(12.36 ±6.04)points](t =0.824,1.436,all P >0.05).The group B had statistically significant differences compared with group A and group C (t =2.689,5.016,3.642,4.382, all P <0.05).The group D also had statistically significant differences compared with group A and group C (t =2.786,4.672,6.415,5.106,all P <0.05 ).There was positive correlation between the IPSS score before and 2 months after operation and the degree of inflammation in group B,D(B:r =0.698,0.795,D:r =0.546,0.485,all P <0.05),but there was no correlation in group C between them(r =0.182,0.170,all P >0.05 ).Conclusion There is a close correlation between LUTS and inflammation around gland in patients with BPH,and the LUTS can aggravate with the increased inflammation around gland and do not relate with the gland inflammation.

Objective To detect the expression of Raf kinase inhibitor protein (RKIP) and NF-κB in renal tissue of diabetic kidney disease (DKD) rats model,and to investigate the effect of rituximab (RTX) on the expression of RKIP in the renal tissue of DKD rats. Methods SD rats were randomly divided into normal group (N),DKD model group (M) and RTX treatment group (D).Blood glucose and 24-hour urine protein of rats were determined in three groups.RKIP protein and NF-κB protein were determined by immunohistochemistry staining. RKIP protein expression was detected by Western blotting. Results Compared with N group,blood glucose,24-hour urine protein and NF-κB expression in M group increased significantly (all P＜ 0.01),the expression of RKIP in M group decreased significantly (P＜0.05).Compared with M group,the expression of RKIP increased significantly in D group (P＜0.05),and 24-hour urine protein and NF-κB expression decreased in D group (all P＜0.05).NF-κB protein expression was negatively correlated with RKIP expression in M group. Conclusions The NF-κB pathway regulated by RKIP plays an important role in the development and pathogenesis of diabetic nephropathy.Rituximab may have a role in treatment of DKD.

Objective To investigate the role of psychotherapy plays in the treatment of vitiligo.Methods Psychological health questionnaire(SCL-90)was used in the study to evaluate the psychological well being of 120 vitiligo patients.66 subjects suffering from psychological problems were divided randomly into groups Ⅰand Ⅱ while 54 subjects free of psychological problems randomly into groups Ⅲ and Ⅳ.Psychotherapy was applied together with drug and phototherapy to those subjects from groups Ⅰ and Ⅲ while groups Ⅱ and Ⅳ having no psychotherapy accompanied.After 3 months of treatment,evaluation was done on the treatment effect with psychological testing once again.The correlation between the improvement of psychological conditions of the subjects and the lesion development was then evaluated by"P"value of the four groups.Results Significant difference was achieved between group Ⅰ and group I1I(P＜0.05).There was no significant difference between groupsⅢand IV(P＞0.05).There was significant difference between groups Ⅰ and Ⅱ.There was no significant difference between groups Ⅱ and Ⅳ.Conclusions The psychotherapy is helpful in improving the early treatment effect and quality of life in vitiligo patients.

Objective:To analyze the levels of arachidonic acid (AA)and docosahexaenoic acid (DHA)in the breast milk of lactating mothers in Changchun City of Jilin Province, and to explore their influence factors. Methods:The lactating mother’s basic information was collected with questionnaire, and the breast milk of lactating mothers on postpartum 22-25 d was obtained and the 3-day 24-hour dietary recall method was used to investigate the dietary intake information of 514 healthy lactating mothers.The Food Composition Table of China 2009 was used to calculate the intakes of five kinds of polyunsaturated fatty acids (PUFAs) in diet of lactating mothers and the gold key nutrition expert system software for corresponding nutrient analysis was used to calculate the amount of various kinds of foods in the lactating mothers’daily diet.The levels of AA and DHA in breast milk were determined with gas chromatography and the linear regression was used to analyze the related factors of AA and DHA levels in breast milk.Results:①The average concentration of AA in breast milk of 514 lactating mothers was (0.08±0.04)g·100 g-1,and the average concentration of DHA was (0.05±0.04)g·100 g-1. ②The single factor correlation analysis results showed that the oil intake was both positively correlated with AA and DHA levels in milk of lactating mothers (r= 0.360,r=0.354,P<0.001),while the intakes of linoleic acid (LA), alpha linolenic acid (ALA),eicosapentaenoic acid (EPA),DHA,dairy and meats and seafood in diet were both negatively correlated with AA (r= -0.321,r=-0.280,r=-0.255,r=-0.299,r=-0.196 ,r=-0.306,P<0.05)and DHA (r=-0.315,r=-0.279,r=-0.175,r=-0.189,r=-0.248,r=-0.142,P<0.05).③The linear regression analysis results showed regression equation that dairy intake (β=-0.265)and EPA intake (β=-0.144)were both negatively correlated with the level of AA (P=0.009),and dairy intake was also negatively correlated with the level of DHA (β=-0.233,P<0.001).Conclusion:The AA and DHA levels in breast milk of lactating mothers didn’t increase with the increasing of intake of milk or dairy products in the study.Moreover there is a competitive relationship between n-6 and n-3 series polyunsaturated fatty acids in the process of metabolism.

Objective To investigate the association between rs2281951 and rs3798753 in ELOVL fatty acid elongase2 (ELOVL2 gene)and the docosahexenoic acid (DHA)level in breast milk,and to clarify the influence of the polymorphisms of ELOVL2 gene in the DHA level of breast milk.Methods 209 healthy maternals were selected and signed the consent form and completed the 3-day 24-hour dietary recall questionaire on one day during the 22nd-the 25th day after partum,and 20 mL breast milk was collected.The DHA level in breast milk was detected with gas chromatography.The milk DNA was extracted and two single nucleotide polymorphisms (SNPs) of ELOVL2 gene were detected by Sequenom Mass Array System. UNPHASED 3.012 genetics software was adopted to analyze the quantitative trait of haplotype and the DHA level in breast milk.Results The distribution of genotypic frequencies of rs2281591 and rs3798713 sites in ELOVL2 gene was consistent with Hardy-Weinberg equilibrium (P > 0.05).The dietary fatty acid intakes and the milk DHA levels of maternals carrying different genotypes had no statistically significant differences (P > 0.05 ). The DHA levels in breast milk of maternals carring different rs3798713 (CG)-rs2281591 (AG)haplotypes had no statistically significant difference (χ2 =3.422,df =5,P =0.635).Conclusion Rs3798713 and rs2281951 and constructed haplotypes in ELOVL2 gene are not related to the DHA levels in breast milk.

OBJECTIVE To evaluate the effect of antiviral agents in treating of severe acute respiratory syndrome(SARS). METHODS Based on the particular data (including medical history,examiuations and treatments) of 1702 patients of with SARS which were offered by the Health Bureau of Guangdong Province,the inpact factors of death and the course of diseases of SARS were retrospectively analyzed with unifactorial and multifactors statistic analysis methods. RESULTS It did not show any signs that the death rate could be decreased by the six antiviral agents in common use,but vidarabine could help to shorten the course of disease(OR=1.399,P=0.025),while oseltamivir might be prolonged the course of disease(OR=0.708,P=0.000). CONCLUSIONS The data showed that the effect of the six antiviral agents for curing SARS is limited,and a new antiviral treatment for SARS should be explored further.

Objective To study the sonograms of tuberculous peritonitis and improve the diagnostic accuracy of this disease.Methods We retrispectively analyzed 138 cases of tuberculous peritonitis diagnosed by ultrasonically guided biopsy,postoperative pathology,and clinical manifestation.The characteristics of the sonograms were analyzed,including the amount and character of ascites,the changes in greater omentum,peritoneum,and mesenterium.the enlarged celiac lymph nodes,and the organ involvement.Results The sonograms of tuberculous peritonitis were characterized by thickening of greater omentum with different degrees of ascites,which usually presented cerebral fissure sign under high-frequency ultrasound,together with aggregation of intestines,thickening of peritoneum,enlarged celiac lymph nodes,hepatomegaly,splenomegaly,and pleural effusion.Conclusion Ultrasonography is of great clinical significance in the diagnosis of tuberculous peritonitis and the guidance of greater omentum biopsy.