Objective:To explore the value of combined application of T2WI, diffusion weighted imaging (DWI) and magnetic resonance dynamic enhanced scanning (DCE-MRI) in the diagnosis of prostate cancer (PCa).Methods:A total of 50 PCa patients received in the First People's Hospital of Pinghu from March 2015 to August 2018 were selected as the PCa group, 54 patients with benign prostatic hyperplasia (BPH) in the same period were selected as the BPH group for a cross-sectional study. Both groups of patients underwent conventional MR scans. The apparent diffusion coefficient (ADC), exponential apparent diffusion coefficient (EADC), peak time (Tmax), peak intensity (SIpeak), signal enhancement rate (SER), maximum enhancement slope (MSI) and time signal intensity (TIC) curve types were compared between the two groups, and the differences between the cancerous and non cancerous foci were analyzed comprehensively. The results of T2WI, DWI and DCE-MRI were statistically analyzed, and the diagnostic efficacy of the three methods alone and combined in the diagnosis of PCA were compared.Results:The ADC value of PCa group was lower than that of BPH group, and the EADC value was higher than that of BPH group ( P<0.05); the Tmax value of the PCa group was lower than that of the BPH group, and the SER and MSI values were higher than those of the BPH group ( P<0.05); the proportion of type Ⅲ TIC curves in the PCa group (64.00%) was higher than that in the BPH group (14.81%), and the proportion of type Ⅱ TIC curves (24.00%) was lower than in the BPH group (66.67%, P<0.05); Conventional T2WI sequence diagnosed 32 cases of cancer, and 10 cases of BPH patients detected low signal in peripheral zone; DWI detected 34 cases of cancer, and 10 cases of BPH patients detected slightly high signal nodules in central gland area, ADC signal was slightly low, and 6 cases of hyperplastic peripheral zone high signal patchy shadow; DCE-MRI showed early obvious enhancement in 42 cases of 50 cases of PCA patients, and decreased rapidly or slowly, and 2 cases showed low peak in 54 cases of BPH, 10 cases of T2WI detected low signal area; 16 cases of DWI detected slightly high signal nodules, of which 8 cases of ADC showed slightly low signal; 46 cases showed early obvious enhancement and gradually increased, or showed platform enhancement; 8 cases of central proliferative nodules showed early obvious enhancement and early enhancement. The sensitivity (96.00%) and accuracy (92.31%) of T2WI, DWI and DCE-MRI in the diagnosis of PCA were higher than those of the three methods alone ( P<0.05). Conclusions:T2WI, DWI, DCE-MRI combined examination can obtain more comprehensive lesion information, significantly improve the sensitivity and accuracy of PCa diagnosis, and help to reduce missed diagnosis.

OBJECTIVE To find out the situation of the usage of antimicrobials in type Ⅰand type Ⅱ operations during perioperative period and to evaluate the rationality of use. METHODS To check 472 examples of medical record data of discharged patients with type Ⅰand type Ⅱ operations in each department during from Apr to Jun and from Aug to Oct 2006,and make statistics and analysis according to application rate of antibacterial drugs, examining rate of the samples, drug usage by experiences and postoperative infection rate. RESULTS The application rate of antibacterial drugs during perioperative period in our hospital was high;the examining rate of correlated samples was low;the choice of antibacterial drugs mainly depended on experience;the sensitivity of the chosen antibacterial drugs was not strong;the duration of prophylactic drug usage was too long;and the postoperative infection rate was high. CONCLUSIONS It is very important to strengthen standardized administration of prophylactic antibiotic usage during perioperative period in our hospital.

Objective To explore the validity and reliability of Nursing Professional Values Scale in registered nurses.Methods Demographic questionnaires and the Revised Nursing Professional Values Scale were used among 280 registered nurses.Results The Cronbach's α was 0.94.The factor analysis extracted 4 factors which could account for 59.55％ variances.These four factors were defined as professional characteristic,providing care,behaviorist,and trust.The Cronbach's α for these four factors were 0.89,0.87,0.86 and 0.70 respectively.Conclusions The Revised Nursing Professional Values Scale possesses desirable validity and reliability in registered nurses,and is a psychometric good instrument of professional value.

Purpose　 The aim is to establish the HPLC method for the determination of Fluoro-deoxyuridine in plasma.Methods　The Chromatography conditions include: Chromatography column: Nova-pak C18(3.9mm×150mm,4μm), mobile phase: 0.05mol/L sodium phosphate monobasic -methanol-water(0.5∶7∶92.5)， UV detection at 260nm， FUDR was extracted with ethyl acetate. Results　The average recoveries were 96.4%，96.5%，97.8% for concentration 0.23、1.67、20.0μg/ml (n=5).The corresponding reproducibility were RSD 1.61%, 1.98%, 3.17% respectwely for iner-day and RSD 3.56%, 1.90%, 2.63% for the intra-day(n=5). The FUDR concentration was linear with a correlation coefficient of 0.999　4 over the range of 0.099～20.0μg/ml. Conclusion　 The method was sensitive and accurate and suitable for pharmacokinetics and bioavailability study of FUDR.

OBJECTIVE: To observe the effect of electro-acupuncture of Neiguan (PC 6) on mean systemic arterial blood pressure and plasmic concentrations of NO and TNFalpha in endotoxin shock rats. METHODS: The model of endotoxin shock was induced by lipopolysaccharide (1.5 mg/kg i.v.) and D-galactosamine (100 mg/kg i.p.). Aminoguanidine (100 mg/kg i.p.) and electro-acupuncture of bilateral Neiguan (PC 6) were administered. A catheter was inserted into the right subclavian artery to record the change of blood pressure and the blood was abstracted out and centrifuged to determine the NO and TNFalpha concentrations. RESULTS: Electro-acupuncture of Neiguan (PC 6) retrieved the blood pressure and reduced the plasmic NO and TNFalpha concentrations. CONCLUSION: Electro-acupuncture of Neiguan (PC 6) expresses an anti-endotoxin shock effect by repressing the plasmic NO and TNFalpha concentrations smoothly and retrieving the blood pressure stably.

Objective To evaluate the diagnostic value of the combination of serum DKK1 (Dickkopf-1 )and P53 autoantibodies in patients with esophageal squamous cell carcinoma (ESCC).Methods Serum levels of DKK1 and P53 autoantibodies were measured by enzyme-linked immunosorbent assay (ELISA) for the 1 26 patients with ESCC and 60 normal controls.Receiver operating characteristics (ROC)was used to calculate the diagnostic efficiency.Results The serum levels of DKK1 and P53 autoantibodies were signifi-cantly higher in ESCC than those in normal controls [(673.09 ±343.82)pg/ml vs (362.05 ±1 48.07)pg/ml, Z =6.1 58,P <0.000 1 ;(0.398 ±0.546)vs (0.069 ±0.050),Z =3.832,P <0.000 1 ].ROC curves showed the optimum diagnostic cutoff for serum DKK1 was 588.77 pg/ml,with an area under curve (AUC)of 0.780 (95%CI:0.71 5 ~0.844,61 .9% sensitivity,95.0% specificity).Measurement of P53 autoantibodies demonstrated an AUC of 0.674 (95%CI:0.598 ~0.750,45.3% sensitivity,95.0% specificity).The com-bination of DKK1 and P53 autoantibodies yielded an AUC of 0.843 (95%CI:0.788 ~0.897,73.8% sensitiv-ity,95.0% specificity).In early-stage ESCC,combined detection of DKK1 and P53 autoantibodies improved the diagnostic power,with an AUC of 0.903 (95%CI:0.845 ~0.961 ,81 .0% sensitivity,95.0% specifici-ty).Conclusion Serum DKK1 and P53 autoantibodies can be used as potential diagnostic biomarkers for the ESCC.Combined detection of them might aid the early diagnosis of ESCC.

Objective To explore the effects of electro-acupuncture combined with surface gastric pacing on gastrointestinal dysfunction for patients with spinal cord injury (SCI).Methods Seventy-five SCI patients with gastrointestinal dysfunction were divided randomly into an electro-acupuncture group (group A),a gastric pacing treatment group (group B) and a group for which electro-acupuncture was combined with gastric pacing (group C).There were 25 cases in each group.Members of group A were given 30 minutes of electro-acupuncture per day ; those of group B had 30 minutes of gastric pacing treatment twice per day ; group C was provided with both 30 minutes of electro-acupuncture and one 30-minute gastric pacing treatment per day.All of the interventions lasted ten days.Before and after the treatment,all patients were assessed using a clinical symptoms score and with electrogastrograms,and any adverse effects were observed.Results There was a significant improvement in upper abdominal pain and epigastric discomfort,bloating and early satiety,nausea,vomiting,as well as belching and loss of appetite in each group after treatment.Before treatment there was no significant difference in the average clinical symptom scores of the three groups.After treatment the values had decreased,and those of group C were significantly lower than group A or B.The average scores of groups B and A were not significantly different.The electrogastrogram results showed than after treatment there was significant improvement in N％ and T％ in groups A and B,and also significant improvement in N％,B％ and T％ in group C compared to before treatment.Group C's improvements were all significantly greater than those observed in groups A and B.Conclusion Electro-acupuncture or gastric pacing alone can give some improvement in gastrointestinal function and its electrophysiological indicators,but their combined effects are better than when either is used alone.

Objective To examine the proto_oncoge ne c_f os expression and its distribution in rat medulla oblongata induced by electro_a cupuncture of Neiguan point. Methods Anti_c_fos immunohistochemical method w as applied. Results Fos_like immunoreactive positive neurons mainly located in nucleus tractus solitarius, dorsal motor nucleus of vagus nerve, inferior ol ive nucleus, cuneate nucleus, external cuneate nucleus, and ventrolateral m edulla(VLM). VLM included lateral reticular nucleus, ambiguous nucleus and the s urrou nding reticular structure. Conclusion Electro_acupuncture of Neiguan point c an activate the proprioceptive pathways and the nucleus in the medulla oblongata that is related to visceral informatio n.

Objective To investigate the effect of Plastrum Testudinis on neural stem cell after focal cerebral ischemia.Methods Rat models of focal cerebral ischemia were established by inserting nylon thread into the anterior cerebral artery.Immunohistochemical method was used to detect the expression of the neuroepithelial stem cell protein(Nestin),neurologic deficit and histological features were also observed.Results Plastrum Testudinis can decrease the neurologic dificit score and promote the expression of Nestin.Conclusion Plastrum Testudinis can alleviate the neurological symptoms and promote the proliferation of neural stem cell after focal cerebral ischemia.

[Objective] To investigate the influence of injection of substance P (SP) and its antagonist into locus ceruleus on electroacupuncture of Neiguan for acute myocardial ischemia (AMI). [Methods] Rabbit model of AMI was set up by ligating left ventricular branch of coronary artery. Forty-nine rabbits were allocated to five groups: Group A (the control group), Group B (electroacupuncture of Neiguan), Group C (injection of SP), Group D (electroacupuncture of Neiguan and injection of SP) and Group E (electroacupuncture of Neiguan and injection of SP antagonist) . Locus ceruleus was located by cerebral stereotaxic apparatus. Dynamic changes of ST segment of electrocardiogram and morphological feature of myocardium were observed. [ Results ] Injection of SP promoted the recovery of AMI and enhance the effect of electroacupuncture of Neiguan for AMI. However, the above effects were counteracted by the injection of SP antagonist. [Conclusion] SP plays an important role in reducing the damage of AMI on electroacupuncture of Neiguan and may serve as an important mediator for the connection of Neiguan and the heart.