Objective:To explore the value of combined application of T2WI, diffusion weighted imaging (DWI) and magnetic resonance dynamic enhanced scanning (DCE-MRI) in the diagnosis of prostate cancer (PCa).Methods:A total of 50 PCa patients received in the First People's Hospital of Pinghu from March 2015 to August 2018 were selected as the PCa group, 54 patients with benign prostatic hyperplasia (BPH) in the same period were selected as the BPH group for a cross-sectional study. Both groups of patients underwent conventional MR scans. The apparent diffusion coefficient (ADC), exponential apparent diffusion coefficient (EADC), peak time (Tmax), peak intensity (SIpeak), signal enhancement rate (SER), maximum enhancement slope (MSI) and time signal intensity (TIC) curve types were compared between the two groups, and the differences between the cancerous and non cancerous foci were analyzed comprehensively. The results of T2WI, DWI and DCE-MRI were statistically analyzed, and the diagnostic efficacy of the three methods alone and combined in the diagnosis of PCA were compared.Results:The ADC value of PCa group was lower than that of BPH group, and the EADC value was higher than that of BPH group ( P<0.05); the Tmax value of the PCa group was lower than that of the BPH group, and the SER and MSI values were higher than those of the BPH group ( P<0.05); the proportion of type Ⅲ TIC curves in the PCa group (64.00%) was higher than that in the BPH group (14.81%), and the proportion of type Ⅱ TIC curves (24.00%) was lower than in the BPH group (66.67%, P<0.05); Conventional T2WI sequence diagnosed 32 cases of cancer, and 10 cases of BPH patients detected low signal in peripheral zone; DWI detected 34 cases of cancer, and 10 cases of BPH patients detected slightly high signal nodules in central gland area, ADC signal was slightly low, and 6 cases of hyperplastic peripheral zone high signal patchy shadow; DCE-MRI showed early obvious enhancement in 42 cases of 50 cases of PCA patients, and decreased rapidly or slowly, and 2 cases showed low peak in 54 cases of BPH, 10 cases of T2WI detected low signal area; 16 cases of DWI detected slightly high signal nodules, of which 8 cases of ADC showed slightly low signal; 46 cases showed early obvious enhancement and gradually increased, or showed platform enhancement; 8 cases of central proliferative nodules showed early obvious enhancement and early enhancement. The sensitivity (96.00%) and accuracy (92.31%) of T2WI, DWI and DCE-MRI in the diagnosis of PCA were higher than those of the three methods alone ( P<0.05). Conclusions:T2WI, DWI, DCE-MRI combined examination can obtain more comprehensive lesion information, significantly improve the sensitivity and accuracy of PCa diagnosis, and help to reduce missed diagnosis.

Cardio-pulmonary exercise was performed in 43 normal subjects and 175 patients with cardiac disease in order to evaluate the severity of heart failure. AT could be determined easily during bicycle ergometer exercise by measuring minute ventination (V_E), oxygen uptake (V_(O2)) and carbon dioxide output (V_(CO2)). Peak V_(O2) and % AT were also determined, Both AT(ml/min/kg) and peak V_(O2) (ml/min/kg) declined with age, how ever male showed higher values than femal in both indiceS, % AT (determined by the predicted AT values of each age and sex), decreased as class of NYHA progressed (i. e. 85.3?10.3% in class Ⅰ, 74.7?14.1% in class Ⅱ, and 58.8?12.8% in class Ⅲ). The reproducibility of AT was excellent in two testing With in a 3-hour interval. These results suggested that indices from cardio-pulmonary exercise testing, especially AT, were closely related to the pathophysiology of heart failure so that they might be used as an objective parameters and were reliable for evaluation of the severity of heart failure and are sensitive enough to detect the efficacy of therapuetic intervention for heart failure.

The aim of the current study was to determine whether tumor-specific T cells can be primed in and obtained from sponge implants loaded with tumor associated peptides. Naive C57BL/6 mice as well as C57BL/6 mice previously primed with FBL-3 tumor cells were implanted with small polyurethane sponges containing FBL-3 gag peptides CCLCLTVFL(gPr80 gag 85-93)or RSPTNLAKV(Pr65 gag p30 131-139). Both FBL-3 gag peptides were shown could bind to H-2 Db molecules. Ten days later, cells that had accumulated in the sponges were harvested, stimulated in vitro with the immunizing peptide, and tested for cytolytic activity against FBL-3 tumor and FBL-3 gag peptides. The results demonstrated that peptide-specific CD8+ CTL could be elicited and obtained from the sponge implants of both naive and immune mice. The FBL-3 gag p85-93 peptide induced CTL could specifically lyse syngeneic targets pulsed with the FBL-3 gag p85-93 peptide as well as FBL-3 tumor. However, the FBL-3 gag p131-139 peptide induced CTL lysed only the FBL-3 gag p131- 139 peptide pulsed syngeneic targets but not the FBL- 3 tumor. Tumor-specific T cells obtained from peptide-loaded sponge implants could be induced to grow to large numbers in vitro by periodic restimulation with the immunizing peptide plus syngeneic APC and low concentrations of IL- 2. Adoptive transfer of the resultant expanded FBL-3 gag p85-93 peptide-induced CTL into mice with disseminated FBL-3 could mediate effective anti-tumor therapy. Thus,in vivo immunization with peptide-loaded sponges provides a potentially useful technique for procuring primed peptide- specific T cells for tumor therapy.

Sulfotransferase (SULT) enzymes play important roles in the biosynthesis of estrogen.These enzymes catalyze the sulfation of estrogens to form biologically inactive molecules. Several studies have shown that Arg213His polymorphism in SULT1A1 gene may be a risk factor for breast cancer. In addition, a low mRNA expression of SULT has been significantly associated with reduced recurrence and improved overall survival in patients with estrogen receptor (ER)-positive tumors. Furthermore, SULT may be a crucial factor in the response to tamoxifen treatment. As an important part of estrogen synthetic pathways, SULTs are closely re lated to breast cancer.

It is important and imminent that bilingual teaching in anesthesiology is carried out and promoted in Chinese medical universities.This teaching has been implemented in Chongqing Medical University for ten years.The desired results have been achieved.This paper analyzes the importance of proper attitudes towards bilingual teaching,using the efficient methods,paying more attention to developing the students' ability of autonomous and improving the teachers' education quality.

At present,the commonly used tumor markers,with low sensitivity and low specificity,can 't achieve the purpose of early diagnosis.However,autoantibodies against tumox-associated antigens have been detected in the asymptomatic stage of cancer and thus can serve as biomarkers for early cancer diagnosis.Moreover,due to autoantibodies are found in sera,they can he screened easily using a noninvasive approach.The discovery of panels of autoantibodies with high sensitivity and specificity will help to improve the diagnostic and prognostic for the patients with cancer.

Multidisciplinary team(MDT)diagnosis and treatment of colorectal cancer has been adopted by plenty of hospitals as a kind of effective comprehensive therapy.Compared with the traditional diagnosis and treatment methods,MDT diagnosis and treatment achieve standardization of diagnosis and treatment and technical promotion through specialization of the team to ensure better treatment and prognosis of patients.However,MDT diagnosis and treatment of colorectal cancer is not perfect,which can be limited by two aspects of hospitals and patients and can realize maximum utility with knowledge of its characteristics.This article will try to explore the distribution and usage of the resource in MDT diagnosis and treatment of colorectal cancer,the advantages over the traditional cure methods and the limits.

Gastric cancer is a kind of disease with high incidence and mortality rate,most gastric cancer cases are diagnosed in an advanced,and chemotherapy is the main treatment.Drug resistance is one of the most important causes of therapy failure in gastric cancer patients.It has revealed that the mechanism of tumor resistance mainly involves alterations in cell cycle and proliferation,enhanced DNA repair capacity,defective apoptosis,damaged host immune,altered metabolism of drugs and so on.In recent years,an important molecule-microRNA(miRNA)that regulates the biological behavior of cell has attracted more and more attention.Studies have confirmed that numerous miRNAs can regulate the drug resistance of gastric cancer cells via BCL-2 signaling pathway,PTEN/PI3K/AKT pathway,ABCB1(MDR1/P-gp),autophagy,cell cycle and so on.

Objective To investigate the clinical application value of ultrasound-guided catheter drainage and vacuum-assisted Mammotome system in the treatment of lactational breast abscess. Methods The clinical data of 46 patients with lactational breast abscesses were analyzed retrospectively. All patients were divided into control group (28 patients) and treatment group (18 patients). Homemade irrigation and drainage tube was used in control group,and vacuum-assisted Mammotome system was used in treatment group. The curative effect of 2 groups was compared. Results All patients were cured. The mean healing time in treatment group was (9.5 ± 1.3) d,in control group was (12.5 ± 2.2) d, and there was significant difference (P < 0.01). The rate of breast-feeding in control group was 57.1% (16/28), in treatment group was 13/18,and there was no significant difference (χ2=1.069, P>0.05). All patients recovered well without recurrence. Conclusions Ultrasound-guided catheter drainage should be employed as first line treatment of breast abscess with diameter greater than the 3 cm. If patient′s economic conditions are good and have high requirement on the beauty outcome, vacuum-assisted Mammotome system can be applied as treatment option.

To capture a state of the enzyme in complex with an intact substrate, we developed and adopted a novel freezing method in crystal preparation procedure. Neither the elimination of the catalytically indispensable ligands, nor mutation or modification of the active site is required. At -20 degrees C, we soaked the crystal of 6-phosphate-beta-glucosidase (Bg1A) in the liquor containing p-nitrophenyl-beta-D-glucopyranoside-6-phosphate (pNPbetaG6P). The diffraction data at 2 A resolution was collected and an intact and unambiguous electron density map of pNPbetaG6P was obtained. These results provide an effective method for the research of cryoenzymology and the intermediate state of enzyme-substrate complex in the future.
Cold Temperature ; Crystallization ; Glucosidases ; chemistry ; metabolism ; Substrate Specificity ; X-Ray Diffraction