Objective To study and analyze the application of Humanistic Psychological Intervention in the operation of laparoscopic bladder tumor resection and ileal neobladder. Methods 100 cases of laparoscopic bladder tumor resection and ileal neobladder were selected from February 2015 to December 2016 in our hospital as the study subjects, and randomly divided into the control group and the experimental group, with 50 patients in each group. The patients in control group received routine nursing care, while the experimental group received Humanistic Psychological Intervention Nursing in operation room. The nursing satisfaction and related clinical indicators were compared in the two groups. Results After the corresponding nursing, the total satisfaction in the experimental group was 47 cases (94%), the total satisfaction in the control group was 36(72%). The satisfaction degree of the patients in the experimental group was significantly higher than that in the control group, the difference was statistically significant (P<0.05). The hospitalization time and operation time of the experimental group were significantly shorter than those of the control group, and the amount of bleeding in the control group was significantly higher than that in the experimental group, the difference was statistically significant (P<0.05). Conclusion The application of operation room humanized psychological intervention after resection of ileal neobladder surgery in laparoscopic bladder tumor can improve nursing satisfaction to a large extent, improve the treatment effect of the patients, help patients recover, with further clinical promotion and application significance.

OBJECTIVE:To explore the effects of pharmaceutical care on blood glucose control and medication compliance in patients with diabetes mellitus. METHODS:Nighty-two discharged patients with diabetes mellitus in our hospital from Apr. to Sept. 2015 were divided into intervention group and control group by random number table method,with 46 cases in each group. Both groups were given Chlorpropamide tablets+Metformin glibenclamide tablets(Ⅰ)for 3 months;intervention group additionally re-ceived pharmaceutical care as medication education,telephone follow-up,etc. The levels of glycosylated hemoglobin and blood glu-cose,medication compliance were compared between 2 groups before and after treatment. RESULTS:Before treatment,there was no statistical significance in the levels of glycosylated hemoglobin and blood glucose,or medication compliance between 2 groups (P>0.05). After treatment,the levels of glycosylated hemoglobin and blood glucose in 2 groups were significantly decreased,and the intervention group was significantly lower than control group;the effects of blood glucose control in patients younger than 60 years old were better than in patients older than 60 years old,with statistical significance(P<0.05). After one month of treatment, the proportion of good medication compliance were increased significantly in 2 groups,and the intervention group was significantly higher than control group. After 3 months of treatment,the proportion of good medication compliance in 2 groups were significant-ly higher than before treatment,and 1 month after treatment,while the intervention group was significantly higher than control group. The improvement of medication compliance in patients younger than 60 years old was better than in patients older than 60 years old,with statistical significance (P<0.05). CONCLUSIONS:Pharmaceutical care contributes to blood glucose control and improves the medication compliance,especially for those younger than 60 years old.

OBJECTIVE:To prepare the gene recombined human interferon-alpha poly(lactic acid-co-glycolic)microspheres and study its physicochemical properties.METHODS:Interferon-? microspheres were prepared by W/O/W solvent extraction technique;the in vitro release of microspheres were examined;the rudimental organic solvent was determined with GC-MS.RESULTS:The IFN-?-MS were regular in their morphology,and the particle size distribution was narrow.The average diameter was 45.54?m.The encapsulation rate and the loading efficiency were 83.49% and 8.03%,and the in vitro accumulative release rate was 80.32% in 30 days.The in vitro interferon-? release from the microspheres was best described using Higuchi diffusion model.The rudimental of dichloromethane was less than 0.05%.CONCLUSIONS:The prepared IFN-?-MS is very good in all aspects of its physicochemical properties.

Directing at the cultivating aims of the students in major of basic medical science,the article expounds some instruction innovations in teaching pharmacology.We introduce some social practical problems and some research fields which evolve rapidly to the teaching class by compiling the teaching materials,conducting lectures and giving discussion lessons.In addition,we incorporate the cultivation of students' capability of doing scientific research to the teaching work through guiding them how to read references,directing them to finish independently the project of experiment designed by themselves and operating group activities instructed by their supervisors in the department of pharmacology.

Objective To study the effect of NLK on TGFβsignaling pathway and explore the molecular mechanism .Methods Protein stability assay was used to determine the influence of NLK on the degradation of Smad 4 .In vivo ubiquitination assay was applied to detect the effect of NLK on the ubiquitination of Smad4 .Luciferase reporter gene assay was used to detect the effect of NLK on CAGA‐luc and 3TP‐luc ,the responsive reporter of TGFβ signaling pathway .Real time PCR was applied to examine the effect of NLK on the expression of p21 and PAI‐1 ,the target genes of TGFβsignaling pathway .Results In HEK293T cell ,over ex‐pression of NLK promotes the degradation and ubiquitination of Smad4 .In HEK293T cells ,Ectopic expression of NLK inhibits the activity of CAGA luc and 3TP luc stimulated by TGFβ.In HepG2 cells ,over expression of NLK inhibits the expression of p21 and PAI 1 ,the target genes of TGFβsignaling pathway .Conclusion NLK promotes the ubiquitination and degradation of Smad4 ,conse‐quently inhibits TGFβsignaling pathway .

Objective To evaluate the relationship between hyperuricemia and nonalcoholic fatty liver disease(NAFLD) by observing the prevalence of NAFLD among healthy individuals with different levels of serum uric acid.Methods The data of 5 230 persons from medical centers for health examination were analyzed,such as height,weight,blood pressure,blood lipids,blood sugar, hepatitis-related markers, and abdominal color Doppler ultrasound examination were conducted in the fasting state.The diagnosis of NAFLD was made according to the diagnostic criteria adopted by China Institute of Liver Disease and Alcoholic Liver Disease Group.Results The incidences of overweight or obesity, hypertension,hyperlipidemia, and metabolic syndrome were raised with serum uric acid greater than 333 μmol/L in male and＞233 μmol/L in female subjects(P＜0.05 or P＜0.01). Excluding the metabolic syndrome in male subjects, the incidence of NAFLD was increased with serum uric acid,＞333 μmol/L in males or ＞233 μmol/L in females(P＜0.05 or P＜0.01). In further studies with subjects without any metabolic syndrome, the detection rate of NAFLD was higher in males than in females at the same serum uric acid level(P＜0.01). Logistic regression analysis showed that sex, body mass index, blood glucose, and triglyceride, low-density lipoprotein cholesterol, uric acid grading were risk factors of NAFLD(OR 1.344, 2.500, 1.292, 1.279, 1.244, 1.256 respectively).Conclusion A high serum uric acid level is associated with an increased risk of NAFLD.

Due to the complicated pathogenesis of cardiac arrhythmia, the safe and effective therapeutic strategies for cardiac arrhythmia remain an urgent medical problems in the recent years. In this paper, we introduced the research practice of anti-arrhythmic agents targeting on potassium ion channel. The research progress of anti-arrhythmic agents in up-to-date literatures were also reviewed and prospected.

Objective To investigate the influence of physiological motions on three-dimensional (3D) CT images and the rules of motion artifacts.Methods A mathematical model of motion artifact was set up based on the principle of spiral scanning, hereby the geometrical distortion of 3D image was analyzed.Experiments were carried out with home-made phantoms on a CT scanner.Results The details of 3D images with trivial distortion were legible under static condition.On the contrary, distinct distortion occured under moving condition, and its degree increased with the pitch and motion period. The 3D images were varied with difference of the motion initial phases.Conclusion The distortion of 3D-CT image depends on the motion characters of scanned objects and scanning parameters such as pitch and slice thickness.

OBJECTIVE To enhance the positive rate of the blood culture in order to make pathogenic diagnosis actually and quickly and conducting usage of antimicrobial agents in clinic.To value the clinical applied circumstance of BacT/Alert 3D automated blood culture system.METHODS The 3728 blood cultures were detected by BacT/Alert 3D automated blood culture system,and bacterial susceptibility test was conducted on all isolates using Kirby-Bauer methods with CLSI standards.To statistically analyze the examined time,the positive rate and variety and drug resistance for all kinds of pathogens.RESULTS The blood culture positive rate was 6.0% in the 3728 blood cultures with 222 strains of bacteria.The false positive rate was 0.2% and the false negative rate was 0.4%.The positive rate of blood culture was 0.89% in 12 hours,2.01% in 18 hours,and 3.51% in 24 hours.Among all the 222 isolates,29.7% were Enterobacteriaceae,the drug resistant rates to amikacin,ceftazidime,ciprofloxacin,and imipenem were 13.6%,36.3%,42.4%,and 3.0%,respectively;20.3% were Staphylococcus,the drug resistant rates to erythromycin,levofloxacin,cefoxitin,and vancomycin were 75.6%,33.3%,68.9%,and 0,respectively;11.7% were Enterococcus,the drug resistant rates to errythromycin,levofloxacin,fosfomycin,and vancomycin were 96.2%,80.8%,23.1%,and 0,respectively;11.3% were non-fermented bacilli,the drug resistant rates to amikacin,ceftazidime,ciprofloxacin,and imipenem were 32.0%,52.0%,32.0%,and 32.0%,respectively;12.6% were fungi.CONCLUSIONS The pathogens in the blood specimens are more wider in distribution and more higher in drug resistance rates than before.BacT/Alert 3D automated blood culture system can be an important tool for the blood culture,it can provide the diagnostic help quickly for the clinic and increase the positive rates in blood culture.It can not only shorten the check-up time,but also be more quick and more exact.

OBJECTIVE To understand flora distribution and four antifungal drugs′in vitro antifungal activity of deep fungi in nosocomial infection in order to provide help to clinics.METHODS Fungi were cultured and isolated by routine procedure which identified by VITEK microbe automatic system.Drug susceptibility test used Rosco paper disk diffusion and broth dilution method with NCCLS M27-A.RESULTS Totally 156 strains with 9 species of deep fungi that main fungi were Candida albicans,and C.tropicalis with 57.69%,and 31.41%,respectively,were isolated from nosocomial infection.The major isolating rates of clinical infection specimens were from respiratory,cardiovascular surgery,and neurological departments with 26.28%,12.18%,and 9.62%,respectively.The main infection specimens were from respiratory tract and urinary tract with 71.15% and 16.67%,respectively.Drug resistance rates to fluconazole,amphotericin B,itraconazole,and ketoconazole with Rosco paper disk diffusion were 23.08%,2.56%,12.18%,and 17.36%,MIC90 were 64.0,2.0,8.0,and 16.0mg/L,respectively.CONCLUSIONS The main deep fungi are C.albicans and C.tropicalis.Antifungal activity of amphotericin B is the highest than others.The drug resistance rate to fluconazole is more and more higher.Clinics should use antifungal drug rationally in accordance with drug susceptibility test results.