AIM: To study on the differences of chemical compos i tion between extracts of Bushen recipe prepared by decocting together and single , with fingerprint technology. METHODS: The extracts of Bushen recipe were prepared by decocting together and single under the same process. The comparison was done under the different wavelength s and different brand columns, HPLC method determined the extract's fingerprint within the range of optimized conditions. RESULTS: The ratio of The compositions and its main chemical substa nces are the different between the two extracts, and the extract rate of the main chemical substances are higher in extrat decocted single than in extract decocted togethe r. CONCLUSION: There are some chemical substances and composition's ra tio differences in the extracts of Bushen recipe made by decocting together and single.

Objective To establish a proper extraction method for Yangxue Ruanjian Prescription (YRP) by comparing the chemical component contents and analgesic and anti - inflammatory effect of extracts extracted from YRP by different methods. Methods Water - decocting, alcohol - refluxing and alcohol - percolating methods were adopted and component indexes of paeoniflorin and gentiopicrin were determined by HPLC. The anti - inflammatory effect of the extracts were observed on mice with xylol - induced auricular swelling and analgesic effect by hot - plate method. Results The content of gentiopicrin is similar in the extracts extracted by three methods and the content of paeoniflorin is higher in alcohol - percolating extract than in alcohol - refluxing extract, lowest in water - extraction. The analgesic effect of water - decocting extract is better than that of alcohol - refluxing extract, and that of alcohol - percolating extract is weakest; the anti - inflammatory effect of the three extracts is almost the same. Conclusion Water - decocting extraction is suitable to YRP, and this is determined by the characteristics of Chinese compound prescription.

A new mutant human papiUomavirus type 16 E7 gene, termed HPV16 HBE7, was isolated from cervical carcinoma biopsy samples from patients in an area with high incidence of cervical cancer (Hubei province, China). A previous study showed that the HPVI6 HBE7 protein was primarily cytoplasmic while wild-type HPV16 E7 protein, termed HPV16 WET, was concentrated in the nucleus. With the aim of studying the biological functions of HPV16 HBE7, the transforming potential of HPV16 HBE7 in NIH/3T3 cells was detected through observation of cell morphology, cell proliferation assay and anchorage-independent growth assay. The effect of HPVI6 HBE7 on cell cycle was examined by flow cytometry. Dual-luciferase reporter assay and RT-PCR were used to investigate the influence of HPVI6 HBE7 protein on the expression of regulation factors associated with GI/S checkpoint. The results showed that HPV16 HBE7 protein, as well as HPV16 WE7 protein, held transformation activity. NIH/3T3 cells expressing HPV16 HBE7 could easily transition from G1 phase into S phase and expressed high level of cyclin A and cdc25A. These results indicated HPV16 mutant E7 protein, located in the cytoplasm, induces oncogenic transformation of NIH/3T3 cells via up-regulation of cyclin A and cdc25A.

A reversed phase high-performance liquid chromatography method for the simultaneous analysis of 23 kinds of phenolic components in water extract of porpolis (WEP) was developed. The separation was performed on ZORBAX Eclipse XDB C_(18) column (150 mm×4.6 mm, 5 μm) by gradient elution. The mobile phase consisted of ethanol with 0.1% formic acid at the flow rate of 1.0 mL/min. The detection wavelengths were 256 and 280 nm. The injection volume was 20 μL, and the column temperature was maintained at 35 ℃. The method showed good linear relationship, precision and repeatability. The recoveries were between 93.3% and 106.6%. Eighteen Reference compounds were detected in WEP of Hehei by this method. The content of Catechin was the highest(30.50 mg/g), the next was 3,4-dimethoxycinnamic acid (15.41 mg/g). Nine Reference compounds were detected in WEP of Yunnan. The content of catechin was also the highest (11.23 mg/g), the next was chrysin (15.41 mg/g). Similarity of WEPs between the products of Hebei and Yunnan were 0.099 (256 nm) and 0.194 (280 nm). The chemical compositions of two WEPs were remarkably different.

The etiology underlying ischemic stroke is still elusive. Previous studies have indicated that inflammation might play a key role in the pathogenesis, which provided a novel insight into the therapeutic strategy of ischemic stroke. In this review, we summarize some drugs which regulate the activation and polarization of microglia and further alleviate neurological symptoms.

Objective Study on the reduction of false positive interference in the detection of human immunodeficiency virus(HIV)by chemiluminescence method with polyethylene glycol(PEG)precipitation.Methods A total of 109 outpatients and inpatients in Ningbo Medical Center,Lihuili Hospital from January to June 2022 were selected.The positive samples initially screened by chemiluminescence method were confirmed by Western blotting,and 20 true positive samples and 89 false positive samples were confirmed.According to whether treatment agent was added,the false-positive samples were divided into three groups:no PEG precipitation group(no special treatment,89 cases),PEG precipitation group(PEG precipitation treatment,89 cases)and control group(normal saline treatment,89 cases).The level of HIV antigen/antibody(Ag/Ab)in supernatant was measured after centrifugation,and the difference of HIV Ag/Ab level and recovery rate before and after different treatment methods were compared.Results After intervention,the HIV Ag/Ab concentration in PEG precipitation group and control group was significantly lower than that in non-PEG precipitation group(P<0.001).After intervention,there were 3 positive cases in PEG precipitation group and 56 positive cases in control group,and the positive rates were significantly lower than those in non-PEG precipitation group(P<0.001).The recovery rate of PEG precipitation group was 4.92(2.12,12.69)%,and that of control group was 65.28(18.04,91.28)%.The recovery rate of true positive samples was>50%regardless of PEG treatment or normal saline treatment.Conclusion PEG precipitation method can effectively reduce the false positive interference of chemiluminescent HIV fourth-generation reagent detection,and has no effect on true positive samples.