Objective To investigate the effect of different diluents on the stability of the mixed enzyme-labeled antibody,and screen a suitable diluent for the mixed enzyme-labeled antibody,which can protect the stability of the cocktail mixture of horseradish peroxidase(HRP) conjugated secondary antibody and alkaline phosphatase(AP) conjugated secondary antibody.Methods Using Tris-HCl buffer as the base solution,four different diluent formulations(A,B,C,D) were prepared with different kinds of stabilizers contained in each formula,such as protein,metalion,surfactant and bacteriostatic agent.Mixed enzyme-labeled antibodies were prepared with different diluents and stored at 2～8 ℃ and 37 ℃,which were detected for the stability by ELISA,enzyme activity assay and immunohistochemistry(IHC) staining.Results D solution [Tris(50 mmol/L)+BSA(1.5%,g/100 mL)+Tween-20(0.3%)+Proclin-950(0.1%,g/100 mL)+sodium chloride(150 mmol/L)+L-Ascorbic Acid(1 mmol/L)+L-methionine(5 mmol/L)+L-histidine(5 mmol/L)+sodium caseinate(1%,g/100 mL)+zinc chloride(0.1 mmol/L)+trehalose(8%,g/100 mL)] was of the optimal protective effect.When stored at 37 ℃ for 10 weeks,the working solution of HRP conjugated secondary antibodies and AP conjugated secondary antibodies showed the highest titer,the activity residual ratios of HRP and AP were 93.46% and96.07%,respectively,and there was no significant difference in IHC results.Conclusion This formula diluent can be used for the preparation of mixed enzyme-labeled antibody working solution.

Objective:To prepare for mAb of progesterone receptor. It would provide support for the immunohistochemistry behind. Methods:Target gene connected together with a carrier by seamless cloning method. The target protein that expression by inducing was collected. And with cell fusion method , the monoclonal antibodies were preparation. Then the mAb were detected by IHC. Results: The mAb ( clone 7C7 ) was detected and it found positive for the breast, uterine fibroid tissue, showed negative in colorectal cancer tissue, smooth muscle tissue, the goal of the claim were achieve. Conclusion: Finally, we found the method that prepare for mAb was far beyond our imagination. The result of IHC on different samples about mAb(7C7)obtained compliance with an-ticipation. Study on the difference between the PR-A and PR-B had significance.

Objective: To investigate occupational health status about radiation workers from township hospitals in Gansu, China, and to explore the dose-effect relationship of exposure dose of radiation workers. Methods: A total of 390 X-ray workers from township hospitals in Gansu Province and 450 X-ray workers from the county/district-level hospitals in the county/district where the township hospitals were located were divided into research group and control group. Their workload, radiation protection condition, and occupational health monitoring data were used for impact assessment and risk estimation. Results: The annual workload of radiation workers from township hospitals was 845.33±361.622 people, and that of radiation workers from county/district-level hospitals was 1967.74±762.055 people; there was a significant difference in annual workload between the two groups of subjects (P<0.05) . Indices such as detection rate of lens opacity, detection rate of abnormal platelet count, red blood cell count, and hemoglobin count, micronucleated lymphocyte rate, and chromosome aberration rate were significantly different between the two groups (P<0.05) . Conclusion Radiation workers from township hospitals suffer from certain radiation damage.

Objective:To explore the influencing factors of whole blood cells and genetics of medical radiation workers, and provide technical support for improving occupational health management and strengthening radiation protection.Methods:In January 2022, a total of 4180 medical radiation workers who underwent occupational health examination in Gansu Provincial Center for Disease Control and Prevention from January 2020 to December 2021 were collected as the research objects, and the results of demographic characteristics, whole blood cells, chromosome aberrations, lymphocyte micronucleus and other results were collected. The whole blood cells and genetic abnormalities of different demographic characteristics of medical radiation workers were compared. And the influencing factors of whole blood cells and genetic abnormalities were analyzed by multivariate logistic regression.Results:The rates of hemoglobin (HGB), chromosome aberration and lymphocyte micronucleus abnormality were the highest in the nuclear medicine group, and the rate of white blood cell (WBC) abnormality in the radiotherapy group was higher than those in other occupational groups, the differences were statistically significant ( P<0.05). The abnormal rates of WBC, HGB and lymphocyte micronucleus in female radiation workers were significantly higher than those in male radiation workers ( P<0.001). The abnormal rates of HGB and lymphocyte micronucleus were statistically different among different working years and different age radiation workers ( P<0.001). And the abnormal rate of platelet (PLT) was statistically different among different working years radiation workers ( P<0.05). The abnormal rate of HGB in radiation workers of different hospital levels was statistically different ( P<0.001). Logistic regression analysis showed that the risk of abnormal WBC and HGB in females radiation workers were 3.048 times and 13.122 times of those in males, respectively ( P<0.001). The abnormal risks of WBC in the 6-20 working years group and >20 working years group were 1.517 times and 1.874 times of that in the ≤5 working years group, respectively ( P<0.05). The abnormal risk of PLT in the >20 working years group was 2.643 times of that in ≤5 working years group ( P<0.05). The abnormal risk of WBC in radiotherapy group and intervention group were 2.407 times and 1.341 times of that in general radiotherapy group, respectively ( P<0.05) . Conclusion:Ionizing radiation has different effects on the whole blood cells and genetic indexes of workers in the nuclear medicine, interventional group and radiotherapy group. The occupational health protection of female radiation workers should be paid attention to.

Objective:To explore the influencing factors of whole blood cells and genetics of medical radiation workers, and provide technical support for improving occupational health management and strengthening radiation protection.Methods:In January 2022, a total of 4180 medical radiation workers who underwent occupational health examination in Gansu Provincial Center for Disease Control and Prevention from January 2020 to December 2021 were collected as the research objects, and the results of demographic characteristics, whole blood cells, chromosome aberrations, lymphocyte micronucleus and other results were collected. The whole blood cells and genetic abnormalities of different demographic characteristics of medical radiation workers were compared. And the influencing factors of whole blood cells and genetic abnormalities were analyzed by multivariate logistic regression.Results:The rates of hemoglobin (HGB), chromosome aberration and lymphocyte micronucleus abnormality were the highest in the nuclear medicine group, and the rate of white blood cell (WBC) abnormality in the radiotherapy group was higher than those in other occupational groups, the differences were statistically significant ( P<0.05). The abnormal rates of WBC, HGB and lymphocyte micronucleus in female radiation workers were significantly higher than those in male radiation workers ( P<0.001). The abnormal rates of HGB and lymphocyte micronucleus were statistically different among different working years and different age radiation workers ( P<0.001). And the abnormal rate of platelet (PLT) was statistically different among different working years radiation workers ( P<0.05). The abnormal rate of HGB in radiation workers of different hospital levels was statistically different ( P<0.001). Logistic regression analysis showed that the risk of abnormal WBC and HGB in females radiation workers were 3.048 times and 13.122 times of those in males, respectively ( P<0.001). The abnormal risks of WBC in the 6-20 working years group and >20 working years group were 1.517 times and 1.874 times of that in the ≤5 working years group, respectively ( P<0.05). The abnormal risk of PLT in the >20 working years group was 2.643 times of that in ≤5 working years group ( P<0.05). The abnormal risk of WBC in radiotherapy group and intervention group were 2.407 times and 1.341 times of that in general radiotherapy group, respectively ( P<0.05) . Conclusion:Ionizing radiation has different effects on the whole blood cells and genetic indexes of workers in the nuclear medicine, interventional group and radiotherapy group. The occupational health protection of female radiation workers should be paid attention to.

Objective:To investigate the effects of continuous renal replacement therapy (CRRT) on plasma concentration, clinical efficacy and safety of colistin sulfate.Methods:Clinical data of patients received with colistin sulfate were retrospectively analyzed from our group's previous clinical registration study, which was a prospective, multicenter observation study on the efficacy and pharmacokinetic characteristics of colistin sulfate in patients with severe infection in intensive care unit (ICU). According to whether patients received blood purification treatment, they were divided into CRRT group and non-CRRT group. Baseline data (gender, age, whether complicated with diabetes, chronic nervous system disease, etc), general data (infection of pathogens and sites, steady-state trough concentration, steady-state peak concentration, clinical efficacy, 28-day all-cause mortality, etc) and adverse event (renal injury, nervous system, skin pigmentation, etc) were collected from the two groups.Results:A total of 90 patients were enrolled, including 22 patients in the CRRT group and 68 patients in the non-CRRT group. ① There was no significant difference in gender, age, basic diseases, liver function, infection of pathogens and sites, colistin sulfate dose between the two groups. Compared with the non-CRRT group, the acute physiology and chronic health evaluation Ⅱ (APACHE Ⅱ) and sequential organ failure assessment (SOFA) were higher in the CRRT group [APACHE Ⅱ: 21.77±8.26 vs. 18.01±6.34, P < 0.05; SOFA: 8.5 (7.8, 11.0) vs. 6.0 (4.0, 9.0), P < 0.01], serum creatinine level was higher [μmol/L: 162.0 (119.5, 210.5) vs. 72.0 (52.0, 117.0), P < 0.01]. ② Plasma concentration: there was no significant difference in steady-state trough concentration between CRRT group and non-CRRT group (mg/L: 0.58±0.30 vs. 0.64±0.25, P = 0.328), nor was there significant difference in steady-state peak concentration (mg/L: 1.02±0.37 vs. 1.18±0.45, P = 0.133). ③ Clinical efficacy: there was no significant difference in clinical response rate between CRRT group and non-CRRT group [68.2% (15/22) vs. 80.9% (55/68), P = 0.213]. ④ Safety: acute kidney injury occurred in 2 patients (2.9%) in the non-CRRT group. No obvious neurological symptoms and skin pigmentation were found in the two groups. Conclusions:CRRT had little effect on the elimination of colistin sulfate. Routine blood concentration monitoring (TDM) is warranted in patients received with CRRT.