OBJECTIVE To investigate the mechanism by which Qiaobai cold compress solution （QBCS） improves acne vulgaris （AV） based on transcriptomics and animal experiments. METHODS Rats were randomly divided into a blank control group （ n ＝6） and a modeling group （ n ＝30）. AV models were established in the modeling group by topical application of oleic acid to the inner surface of both ears， combined with subcutaneous injection of Cutibacterium acnes suspension into the auricle. Successfully modeled rats were further divided into the model group， positive control group （Tretinoin cream， 0.045 g/kg）， and QBCS low-， medium-， high-dose groups [3.55， 7.11， 14.22 g/kg （calculated by the amount of crude drug） ] ， with 6 rats in each group. Rats in each d rug group were treated with the corresponding drugs once daily for 14 consecutive days. After the final administration， changes in the appearance of the ears and histopathological changes in the ear tissues were observed， and serum levels of inflammatory factors， including tumor necrosis factor-α （TNF-α）， interleukin-6 （IL-6） and IL-1β， were measured. Auricular tissues from the blank control group， model group and QBCS medium-dose group were collected for transcriptome sequencing. Differential expressed genes （DEGs） were screened and subjected to Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis， followed by validation using real-time quantitative polymerase chain reaction and Western blot assay. RESULTS Compared with the model group， rats in all QBCS groups showed alleviated auricular acne symptoms， with reduced epidermal thickening， sebaceous gland hyperplasia， and inflammatory cell infiltration. Serum levels of TNF-α （except for the QBCS low-dose group）， IL-6 （except for the QBCS low-dose group） and IL-1β were significantly decreased （ P ＜0.05）. A total of 590 DEGs were identified （blank control group vs. model group）， and 596 DEGs were identified （model group vs. QBCS medium-dose group）. Above DEGs （blank control group vs. model group） were mainly enriched in Toll-like receptor （TLR） and nuclear factor-kappa B （NF-κB） signaling pathways， etc. Validation experiments showed that， compared with model group， low-， medium- and high-dose of QBCS reduced， to varying degrees， the mRNA expression of TNF-α， TLR2， interferon-γ and CXC chemokine ligand 8 in the auricular tissues of AV rats， increased the mRNA expression of peroxisome-proliferator-activated receptor gamma and tumor protein 53， and inhibited the phosphorylation of NF-κB p65 protein as well as the expressions of TLR2 and myeloid differentiation primary response protein 88（MyD88） （ P ＜0.05）. CONCLUSIONS QBCS can alleviate auricular inflammation and skin lesions in AV rats. This effect may be related to inhibition of the TLR/MyD88/NF-κB signaling pathway， thereby suppressing the expression of downstream inflammatory factors such as TNF-α.

Perioperative neurocognitive disorders(PND)is a common central nervous system com-plication during the perioperative period.The diagnosis and treatment methods for PND are scarce,and the incidence rate remains high.The pathogenesis and treatment of PND have become one of the future research directions in the perioperative period.Currently,the research shows that disturbance of gut microbiota may be involved in the occurrence and development of PND through the microbiota-gut-brain axis.This article re-views the multiple factors affecting gut microbiota composition during perioperative period,the potential mechanisms of dysbiosis participating in PND,the prediction of the occurrence and development of PND by specific microbiota,and corresponding preventive interventions,so as to provide new concepts for periopera-tive brain health.

Objective To explore the association between ACTN3 gene R577X polymorphism and serum lipid levels in an elderly Chinese Han population.Methods This study was based on the ageing arm of The Rugao Longevity and Ageing Study (RtLAS).Genotyping was performed by Taqman MGB method.Lipids includedtotal cholesterol (TC),triglyceride (TG),high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C).Cut points of dyslipidemia were based on that reported in the NCEP ATPIII.We explored the associations between R577X polymorphism loci and dyslipidemia by using analysis of covariance and logistic regression analysis.Results Overall 1 618 samples was included (741 males and 877 females) in this study.Covariance analysis found that TC level of RR,XX and RX genotype in the general population were 1.34,1.37,1.43 mmol/L with an increasing trend (P =0.024);TC and LDL-C levels of RR,XX and RX genotype (TC:5.13,5.29,5.43 mmol/L,P =0.004;LDL-C:2.76,2.88,3.00 mmol/L,P =0.004) were significantly different in females.Logistic regression analysis showed that the increased copies of X allele were significantly associated with increased TC and LDL-C levels in the general population and females.For the general population,TC:OR=1.184,95％CI:1.030-1.361,P =0.018;LDL-C:OR =1.334,95％CI:1.101-1.588,P=0.003;For females,TC:OR=1.332,95％ CI:1.102-1.616,P =0.003;LDL-C:OR =1.549,95 ％CI:1.208-1.986,P =0.001.After adjusting for other covariates,the above associations remained significant.Conclusions ACTN3 gcnc R577X polymorphism is associated with plasma TC and LDL-C levels in elderly woman of Han populations in Rugao,China.

[Objective]To observe the influence of Yigu oral liquid on inhibiting the pain in ovariectomized rats by detecing the pain threshold, bone densi-ty, serum inflammatory factor, vascular endothelial function(ET) and platelet activating capacity(CD62p and CD63).[Method]80 SD rats were randomly di-vided into four groups of sham group, Yigu oral liquid group, Miacalcic group,control group. There were 20 in each group.Yigu oral liquid group,Mia-calcic group,control group were ovariectomized,sham group underwent sham operation. After confirming the successful model of osteoporosis, sham group was administrated with saline, Miacalcic saline group was administrated with Miacalcic by intramuscular injection ,Yigu oral liquid group was administrated with Yigu oral liquid ,saline and control group was administrated with saline.The indicators of pain threshold,bone density,vascular endothelial function (ET) and platelet activating capacity(CD62p and CD63), serum inflammatory factors as COX-2, PGE2, cAMP were respectively detected. [Results] The levels of peripheral inflammatory factors as COX-2, PGE2, cAMP , vascular endothelial function(ET) and platelet activating capacity(CD62p and CD63)of Yigu oral liquid group decreased significantly compared with the control group( P<0.05). The level of pain threshold and bone density of Yigu oral liquid group improved significantly compared with the control group(P<0.05).[Conclusion]Yigu oral liquid can inhibit osteoporosis pain by improving the level of pain threshold ,bone density,and decreasing the levels of COX-2, PGE2, cAMP, vascular endothelial function(ET) and platelet activating capacity (CD62p and CD63).

Neuron chip is a multiprocessor with three pipeline CPU; its communication protocol and control processor are integrated in effect to carry out the function of communication, control, attemper, I/O, etc. A novel biologic electronic signal measurement network system is composed of intelligent measurement nodes with neuron chip at the core. In this study, the electronic signals such as ECG, EEG, EMG and BOS can be synthetically measured by those intelligent nodes, and some valuable diagnostic messages are found. Wavelet transform is employed in this system to analyze various biologic electronic signals due to its strong time-frequency ability of decomposing signal local character. Better effect is gained. This paper introduces the hardware structure of network and intelligent measurement node, the measurement theory and the signal figure of data acquisition and processing.
Automatic Data Processing ; instrumentation ; Electrocardiography ; instrumentation ; Electroencephalography ; instrumentation ; Electromyography ; instrumentation ; Equipment Design ; Humans ; Monitoring, Physiologic ; instrumentation ; Neurons ; Signal Processing, Computer-Assisted ; instrumentation