Cardiovascular diseases is a common disease and induces high mortality.Blood tests are very important for the diagnosis,prognosis,monitoring and treatment of CVD.Clinicians are frequently presented with laboratory test results that are not consistent with preconceived expectations for a given case.It has been confirmed that pre-analytical quality control may be influenced by factors such as the time of sample collection.In this article different times and styles of sample collection are discussed.

The paper presented the methods and experiences of managing nursing posts in Beijing Friendship Hospital.As introduced in the paper,such measures as conversion of organizational structure,innovative nursing post setup,new training model,dynamic allocation of nursing manpower,and effective performance incentive mechanism,promoted better nursing quality and stabilized nursing team,achieving sustainable development of quality nursing service.

Here we report the clinical,radiological and neuropathological findings of a patient with tumor-like inflammatory demyelinating diseases of the central nervous system.The patient was a 51-year-old man with a four-month history of inflammatory pseudotumor and no other significant medical history,who presented to our hospital recurrent relapse numbness and weakness of his right extremities,dysarthria and memory deterioration.Brain magnetic resonance imaging(MRI) showed mass focal lesion in white matter of left parietal lobes.The biopsy showed numerous infiltrating macrophages and lymphocytes within the perivascular.The patient responded clinically to corticosteroid and intravenous immunoglobulin(IVIG) therapy.According to the results of the biopsy and the MRI,a diagnosis of inflammatory pseu-dotumor of the central nervous system was made.The vascular dysfunction may act in the pathogenesis of inflammatory pseudotumor of the central nervous system.

Objective:To investigate the relationship between the distance from the upper and lower resection margin and the gastric cancer patients with R0 resection and no distant metastasis.Methods:Retrospective analysis of 281 patients with gastric cancer in our hospital,the relationship between the age,sex,tumor size,tumor size,vascular invasion,lymph node metastasis,TNM stage,type of gastric cancer,tumor location and operation mode was analyzed.To compare the survival time of patients with different upper and lower margins.Results:The increase of the distance from upper resection margin was significantly related to the tumor size＞5 cm,TNM stage,type of gastric cancer,tumor location,and the difference was statistically significant(P＜0.05);The distance of lower resection margin was significantly correlated with tumor size＞5cm,vascular invasion,lymph node metastasis and TNM stage,type of gastric cancer,and the difference was statistically significant (P＜0.05);Themedian survival timein patientsof upper resection marginr□3cm with 48 months of the 5-year follow-up period was significantly higher than that in patients of the resection margin＞3cmwith 46 months (P＜0.001).Themedian survival timein patientsof lower resection margin□3cm with 45 months of the 5-year follow-up period was significantly higher than that in patients of the resection margin＞3cm with 44 months (P＜0.001).Conclusion:Gastric cancer postoperative upper and lower resection margin was significantly related with tumor size,TNM staging factors,and the median survival time of upper and lower resection margin＞5 cm was significantly lower than that of the resection margin3 cm.

Objective To investigate the value of Standardized Swallowing Assessment (SSA) in the screening for aspiration after stroke.Methods 81 patients with acute stroke received examinations of both SSA and Fiberoptic Endoscopic Examination of Swallowing (FEES). The SSA was then measured against the FEES study for sensitivity, specificity, positive predictive value and negative predictive value for aspiration in acute stroke patients. The relation between the Results of SSA and pneumonia post-stroke was analyzed.Results Sensitivity, specificity, positive predictive value and negative predictive value of SSA for aspiration were 95.0%, 53.7%, 66.7% and 91.7%, respectively. The patients with positive result on SSA had a higher incidence of pneumonia ( P＜0.05).Conclusion SSA is a valid and valuable screening tool for aspiration in acute stroke patients.

Objective To explore the expression profiles of circulating microRNA(miRNA)and potential markers for the diagnosis of adult fulminant myocarditis(FM). Methods The expression profiles of circulating miRNA were determined by microarray analysis and verified by real-time quantitative PCR.The key role of circulating miRNA in FM was determined via KEGG pathway enrichment.The correlations between miRNA and cardiac function parameters in patients with FM were analyzed.The receiver operating characteristic(ROC)curve was established to evaluate the sensitivity and specificity of circulating miRNA in the diagnosis of FM. Results Compared with healthy controls,the FM patients had up-regulated expression levels of miR-29b(t=18.925,P<0.001)and miR-125b(t=5.981,P=0.029)in the plasma.After treatment,the expression levels of miR-29b(t=12.943,P<0.001)and miR-125b(t=14.016,P<0.001)were significantly down-regulated.KEGG pathway enrichment showed that the targets of miR-29b were involved in inflammatory response and apoptosis pathways.The results of cell proliferation and apoptosis assay demonstrated the transfection of miR-29b mimic had a more significant inducing effect on cardiomyocyte apoptosis than that of miR-125b mimic(χ ²=6.168,P=0.047),whereas there was no significant difference in the inhibition of cell proliferation between the two groups(χ²=1.452,P=0.417).The expression levels of miR-29b and miR-125b were negatively correlated with left ventricular ejection fraction(r=-0.67,P=0.071;r=-0.49,P=0.003).They were positively correlated with cardiac troponin I level(r=0.61,P=0.019;r=0.52,P=0.016),interferon β level(r=0.42,P=0.014;r=0.36,P=0.021),and myocardial edema area(r=0.86,P=0.005;r=0.73,P=0.013).The ROC curve analysis demonstrated that miR-29b had higher sensitivity for the diagnosis of FM(93.6% vs.89.2%;t=0.896,P=0.795)and specificity(72.4% vs.59.6%;t=9.478,P=0.002)than miR-125b. Conclusion The circulating miR-29b may be a potential biomarker for the diagnosis of FM.
Adult ; Biomarkers/metabolism* ; Circulating MicroRNA/metabolism* ; Humans ; MicroRNAs/metabolism* ; Myocarditis/diagnosis* ; Stroke Volume ; Ventricular Function, Left

Objective To investigate the relationship between a novel polymorphism of C5L2 gene and type 2 diabetes mellitus (T2DM) in Uygur population from Xinjiang region.Methods A novel single nucleotide polymorphism(SNP),698C＞T(P233L) was found using a polymerase chain reaction direct-sequencing method.C5L2 gene 698C ＞ T variant from 252 patients with T2DM and 747 healthy control subjects was detected by polymerase chain reaction and restriction fragment length polymorphism.Result Heterozygote carriers of the 698CT genotype were more frequent among T2DM patients than that among controls (0.107 vs 0.036,x2 =18.576,P＜0.01) in the Uygur population. After adjustment of confounding factors such as sex,age,smoking,alcohol consumption,and hypertension,as well as serum levels of triglyceride,total cholesterol,low-density lipoproteincholesterol,and high-density lipoprotein-cholesterol,the difference remained significant ( P＜0.01,OR =3.373,95％ CI 1.736-6.553 ).Conclusion The CT genotype of the C5L2 gene might be a risk factor of T2DM in Uygur nationality population in Xinjiang.

Objecflve To determine the prevalences of nutritional risk,undemutrition,overweight,and obesity as well as nutritional support and the changes of nutritional risks from admission to discharge or over a two-week period.Methods A consecutive sampling was performed.Dam were collected from general surgical depart-ments in three Beijing teaching hospitals from March to July in 2007.Patients were screened using Nutritional Risk Screening 2002(NRS2002)on admission and two weeks after admission(or discharge).The nutritional supper apphcation during hospital stay was recorded.NRS2002 score≥3 was classified as nutritional risk.BMI<1 8.5kg/m2 with impaired genend condition was defined as undemutrition.Results Among 300 enrolled patients,the NRS2002 WaS completed by 99.0%(297/300)of all patients.The nutritional risk and the prevalence of under_nutrition,overweight,and obesity was 30.0%,8.1%,38.3%,and 9.4%,respectively at admission.Fifty of 90(62.2%)patients who were at nutritional risk received nutritional support while 40 of 210(19.O%)non-risk patients received nutritional support.Especially among major abdominal surgery patients,56 of 90(71.6%)pa-tients who were at nutritional risk received nutritional supper while 35 of 81(43.2%)non-risk pafients received nutritional support.The prevalence of nutritional risk changed from 30.0%to 35.8%(X2=2.271,P=0.132).Conclusions NRS2002 is a feasible nutritional risk screening tool among general surgical pafienm in selected Bei-jing teaching hospitals.Nutritional support is somehow inappropriately apphed in general surgical hospitalized pa-tients.The prevalence of nutritional risk remains unchanged in general surgical patients during hospitalization.

ObjectiveTo explore the application effects of 5A nursing model in patients with chronic heart failure(CHF). MethodsBy purposive sampling, 98 CHF patients hospitalized in the Department of Cardiology in Taikang Yanyuan Rehabilitation Hospital of Beijing from January of 2016 to April of 2017 were selected as participants in this study. In accordance with the random number table, the participants were divided into the control group(n=49) and observation group (n=49). Routine nursing was adopted in the control group while the 5A nursing model was used in the observation group. The two groups were compared in terms of their compliance of treatment and quality of life. ResultsThree months after discharge, the patients in the observation group were better than the control group in treatment compliance with statistical difference (P＜0.05). Upon discharge and three months after discharge, the patients in the observation group achieved lower score than the control group in quality of life with statistical difference (P＜0.05). Conclusions5A nursing model can improve the treatment compliance and quality of life of patients with CHF. It is worthy to be widely applied in clinical practice.

Objective:To explore the influencing factors of working alienation among in-service nurses in Beijing, and provide a management basis for clinical nursing managers.Methods:According to the data from the National Bureau of Statistics in 2018, the convenience sampling method was adopted to select two Class Ⅲ hospitals, three ClassⅡ hospitals, and three ClassⅠ hospitals in Beijing from November 2019 to February 2020. Then through stratified sampling, each hospital nursing unit was randomly selected according to a sampling ratio of 20%. Finally, according to the cluster sampling, all the nurses included in the selected nursing unit were finally selected, including 531 ClassⅢ hospital nurses, 394 ClassⅡ hospital nurses, and 60 ClassⅠ hospital nurses, for a total of 985. The survey was conducted with the General Information Questionnaire, Work Alienation Scale, Person-Organization Fit Perceptions Scale and Organizational Support Perceptions Scale. Single factor analysis was used to carry out the influence analysis of general data, including t-test of independent samples, one-way analysis of variance and the non-parametric test of independent samples. Pearson correlation analysis was used to test the correlation among variables, and hierarchical linear regression analysis was used to explore the influencing factors of nurses' sense of work alienation. Finally, 953 valid questionnaires were obtained, with an effective recovery rate of 96.75%. Results:Among 953 nurses, the highest score of work alienation was 88, and the lowest score was 20, the total average score was (45.52±14.96) , which is in the middle level. The highest score of nurse personal-organization fit was 55, the lowest score was 18, and the total average score was (37.37±8.00) ; and the highest score of nurses' organization support perception was 80, the lowest score was 23, and the total average score was (52.79±12.12) , and the nurses' personal-organization fit and organizational support perception were both at the middle to high level. The univariate analysis showed that there were statistical differences in the scores of work alienation among 953 nurses with different ages, nursing ages, departments, technical titles, personnel relationships, and hospital levels ( P<0.05) . The results of Pearson correlation analysis showed that the total score and the scores of four dimensions of work alienation were negatively correlated with the scores of personal-organization fit and organizational support perception with a statistical difference ( P<0.01) . Personal-organization fit and organizational support were positively correlated ( P<0.01) , the difference was also statistical. In hierarchical linear regression analysis, personal-organization fit and organizational support were the main factors affecting nurses' sense of work alienation with a statistical difference ( P<0.05) . Conclusions:The sense of work alienation among nurses in Beijing is at a moderate level. Increasing organizational care can satisfy nurses' personal needs and make them willing to link their career planning with organizational vision to achieve common goals, thereby enhancing their emotional commitment to the organization and willingness to stay, and reducing their sense of work alienation.