1.Acquisition of X-ray image and its format conversion into BMP ones based on CCD
Chinese Medical Equipment Journal 1989;0(03):-
This paper is to introduce the method in which the X-ray images on CCD are converted into BMP ones based on the correct format analysis and identification of X-ray images. The conversion can be realized through software design.
2.The model investigation of digital dental clinic information system
Chinese Medical Equipment Journal 1989;0(01):-
In this paper, the system model of digital dental clinic information system is introduced. The operating flow of dental clinic analyzed, the steps and key technologies for developing digital dental clinic are also discussed.
3.Determination of omeprazole with a new flow-injection chemiluminescence method
Journal of Xi'an Jiaotong University(Medical Sciences) 1981;0(03):-
Objective To develop a new flow-injection chemiluminescence(FI-CL) method for the determination of omeprazole.Methods Strong chemiluminescence(CL) signal was found generated when omeprazole was injected into the reaction mixture of KMnO4 and luminol.The experimental conditions that affected the CL reaction were optimized by using FI mode.Results Under the optimum experimental conditions(150cm of mixing tubing,0.15mol/L NaOH,7.0?10-5mol/L luminol,2.0?10-5mol/L KMnO4 and 2.8mL/min of flow rate),CL intensity was linearly related to the concentration within the range of 2.0?10-8-1.0?10-5g/mL.The detection limit was 3?10-9g/mL omeprazole,and the relative standard deviations(n=11) were 1.0% and 2.6% for 1.0?10-7g/mL and 1.0?10-6g/mL omeprazole solutions,respectively.Conclusion The proposed method is simple,rapid,sensitive,and suitable for determining omeprazole content in pharmaceutical preparations.
4.Research progress of extraction and purification of Hirudin
Sheng HUANG ; Wei XIONG ; Junjie YAO ; Lu LI ; Hongdiao CHEN ; Yinhuan YANG ; Hongjun YAO
Chinese Journal of Biochemical Pharmaceutics 2017;37(2):313-315
Hirudin as a novel anticoagulant and antithromotic agent, is a kind of protein that is obtained from the salivary gland secretion of one kind blood-sucking leech.It increases the difficulty extraction and purification of hirudin that composition of no anticoagulant activity of peseudo-hirudin in the body of leech and a large number of complex material from the salivary gland secretion of one kind blood-sucking leech.This article focuses on reviewing the structure of hirudin, genetic makeup, extraction and purification of hirudin according to the related research.Analysis of the pros and cons of various extraction and purification methods.Looking forward to its developing tendency, so as to provide reference for extraction and purification of Hirudin.
5.BQ123 Alleviates Lung Injury Following Limb Ischemia-Reperfusion in Rats
Yan LIU ; Haibing PENG ; Jianhui WANG ; Yinhuan WANG ; Na ZHANG ; Ying LI ; Liyan ZHU ; Xiuhong YANG ; Lianyuan ZHANG
Herald of Medicine 2014;(9):1129-1131
Objective To observe BQ123 on lung tissue injury following ischemia-reperfusion of hind limbs in rats.Methods A total of 30 male SD rats were randomly divided into control,I-R,and BQ123 groups,with 10 rats in each group. After 4- hour of ischemia and 4-hour of reperfusion to the hind limbs, ET-1 was measured by radioimmunoassay, levels of myeloperoxidase (MPO) and malondialehyde (MDA) were examined by biochemical method,and the content of P-selectin was examined by ELISA. Immunohistochemical method was used to detect the expressions of Fas,Bcl-2 and Caspase-3 in lung tissues. Pulmonary apoptosis was examined by means of TdT-mediated dUTP nick end labeling ( TUNEL) . Results Compared with the control group,the levels of ET-1,MPO,MDA and P-selectin in lung tissues were all increased significantly in I-R group ( P<0. 01). The expressions of Fas,Bcl-2 and Caspase-3 were 0. 294±0. 003,0. 108±0. 005,and 0. 174±0. 003,significantly up-regulated in the I-R group. The apoptosis rate [(18. 83±2. 86)%] was significantly increased in the I-R group (P<0. 01). Compared with I-R group,the tissue contents of ET-1,MPO,MDA and P-selectin were significantly lowered in BQ123 group (P<0. 01). The expression levels of Fas (0. 115±0. 007) and Caspase-3 (0. 159±0. 006) were decreased,but the expression of Bcl-2 was increased (0. 128±0. 005). The apoptosis rate in BQ123 group was significantly lower [(10. 67±2. 16)%,P<0. 01].Conclusion BQ123 may have protective effects on lung tissue after limb ischemia-reperfusion in rats by means of improving neutrophil aggregation and reducing the expression of proteins related to cell apoptosis.