Objective To study the regulatory effect of interleukin-17 on the production of inflammation and bone erosion related factors of fibroblast-like synoviocytes (FLSs) from rheumatoid arthritis (RA) patients and to investigate whether signal transducer and activator of transcription 3 (STAT3) is implicated in this regulatory effect.Methods FLSs were acquired through primary culture from RA patients.Receptor activator for nuclear factor-κB ligand (RANKL) and osteoprotegerin (OPG) expression in FLSs were determined using western blot analysis.The level of IL-6,granulocyte-mac rop hage colony stimulating fac tor (GM-CSF)and IL-8 were determined by enzyme linked immunosorbent assay (ELISA) assay.Statistical product and service solutions (SPSS) 17.0 statistical software was used for single factor analysis of variance and LSD-t comparison between each two groups.Results IL-17 stimulated FLSs and promoted the release of IL-6,GM-CSF and IL-8 (t=2.135,P＜0.01) and increased the production of RANKL (t=2.105,P＜0.01) and reduced the production of OPG (t=2.502,P＜0.01).However,targeted silencing of STAT3 could inhibit the effect of IL-17 on FLSs [control group:IL-6 (512±18) pg/ml;GM-CSF (591±25) pg/ml;IL-8 (473±10) pg/ml;RANKL (2.310±0.096);OPG (0.614±0.311);IL-17 group:IL-6 (1 630±39) pg/ml;GM-CSF (1 825±18) pg/ml;IL-8 (2 804±41) pg/ml;RANKL (4.010±0.256);OPG (0.131±0.101);STAT3 silent group:IL-6 (405±21) pg/ml;GM-CSF (451±12) pg/ml;IL-8 (370±9) pg/ml;RANKL (0.850±0.298);OPG (1.120±0.342)].Conclusion STAT3 pathway is critical to IL-17-induced regulatory effect on the release of inflammation and bone erosion related factors of FLSs.IL-17 can induce the inflammation and bone erosion of RA via STAT3.

Objective To study the effects of acute cadmium exposure on the immune function of white blood cells (WBC), plasma interleukin-2 (IL-2) and cortisol levels in rats. Methods Thirty-six male rats were randomly divided into three groups averagely. The control group (group C) and two experimental groups(groupⅠand group Ⅱ) were respectively exposed to 0, 0.5, 1.0 mg / kg body weight (BW) cadmium for 7 days by intraperitoneal injection. Blood samples of the rats were collected on the 4th and 7th day after administration of cadmium respectively and the related parameters were analyzed. Results The BW of rats in groupⅠand group Ⅱ were significantly lower than that of the group C. The WBC counts of two experimental groups were higher than that of the control group. Higher percentage of neutrophiles and lower percentage of lymphocytes were observed in rats of group Ⅰ and Ⅱ on the 7th day after cadmium exposure, while no obvious variations in monocytes(%), eosinophiles(%) and basophiles(%)were observed among the three groups. Blood T-lymphocyte(%) and IL-2 levels in rats of groupⅠandⅡdecreased on the 4th and 7th day after cadmium exposure respectively, while B-lymphocyte(%) increased on the contrary. Plasma cortisol levels in rats of groupⅠ and groupⅡ were higher than that of the group C on the 7th day after cadmium exposure. Conclusion The results showed that acute cadmium exposure could affect the WBC immune function and result in the defect of cellular immune function as well as significant change of adrenal cortex endocrine activities.

Task-driven teaching method is a kind of exploration.The prospective research on the application of task-driven teaching method in social medical insurance course is helpful to setting up a teaching situation,constructing a relaxed study environment,stiring up students’interest in the study and improving students’initiatives and creativity.

The famous veteran doctors of traditional Chinese medicine ( TCM ) are models to combine TCM the-ories , previous practitioners' clinical experiences with current medical practice for the difficult to solve clinical problems . The selection of appropriate inheritance study method is especially important in the learning of fa-mous veteran doctors of TCM . This article discussed the inheritance method of academic experiences of famous veteran doctor of TCM professor Huang Yongsheng from four aspects, which were the reading of classic books, clinical practice, following medical masters, and summarization of clinical cases. It was hoped that during the inheritance process , efforts can be made to academic characteristics of TCM itself in accordance with its own law in order to create more and better clinical experience inheritance method.

The overall concept is the core theory of traditional Chinese medicine ( TCM ) diagnosis and treat-ment system. During the process of building TCM effect evaluation on coronary heart disease (CHD), with the defined connotation and extension of heart diseases , except the consideration on its clinical symptoms , TCM four diagnosis and various current modern cardiovascular disease diagnostic methods should be organically com-bined according to the original macro basis . The micro quantitative evaluation is given on therapeutic effect of disease treatment in order to construct the complete TCM effect evaluation system.

Objective To explore the effect of rehabilitation training on learning and memorial ability and long-term potentiation (LTP) in hippocampal region CA3 in rats with cerebral infarction. Methods 30 male rats were randomly divide into model group, rehabilitation training group and normal group with 10 cases in each group. Photochemical method was used to induce hippocampal infarction. The performance of Y-maze test and moving-back with current stimulation experiment were recorded. The increase rate of population spike (PS) in hippocampal region CA3 and PS latent period were compared. Results The frequency of training of Y-maze test was less after rehabilitation training, the latent period of moving-back with current stimulation experiment prolonged, and the PS latent period was shortened. Conclusion Rehabilitation training may strengthen the LTP effect in hippocampal region CA3, and improve the learning efficiency

Objective:Optimising the technological parameters for the extraction of Maxingshigan Decoction (Herba Ephedrae, Semen Pruni Armeniacae, Gypsum Fibrosum, Radix Glycyrrhizae). Methods:Using the even design and TCL, ephedrine, glycyrrhetinic acid, hydrocyanic acid, calciumion and extract weight were taken as the indexes. Results:Optimal parameters: the pH values of the water for the 1st, 2nd and 3rd decoctions are 2.0 ,6.5 and 9.0,respectively; and the time of 3 decoctions are 2.0,1.0and 0.5h, respectively. Conclusion:Optimal parameters for Maximpshipan Decoction are feasible in extraction.

PurposeTo investigate the correlation between iodine concentration and clinical severity of acute pancreatitis (AP) through the quantitative evaluation with dual-energy spectral computed tomography (DESCT), so as to find out an effective imaging technology in the evaluation of clinical severity of AP.Materials and Methods Sixty patients with AP confirmed clinically (AP group) and 30 patients with normal pancreas (control group) were retrospectively analyzed. All the patients underwent enhanced CT scan in the spectral imaging mode. Iodine concentration and normalized iodine concentration (NIC) were respectively measured during arterial phase and portal phase in the material-decomposition images by using a spectral imaging viewer (GSI Viewer). Results Iodine concentration and NIC were significantly higher in the control group than in the AP group (P<0.05,P<0.001). In the AP group, according to Ranson grading, 24 patients were in the mild grade, 20 patients were moderate, and 16 patients were severe. Iodine concentration and NIC decreased along with the increase of their Ranson grade. There were significant difference in iodine concentration and NIC among the three subgroups (iodine concentration on arterial phase:F=8.776,P<0.01; iodine concentration on portal phase:F=4.019,P<0.05; NIC on arterial phase:F=12.700,P<0.001; NIC on portal phase:F=8.732,P<0.01). Iodine concentration and NIC on arterial and portal phases in the mild grade group were both significantly higher than those in the moderate grade group (P<0.05); however, iodine concentration on arterial and portal phases, and NIC on arterial phase in the severe grade group were significantly lower than those in the moderate grade group (P<0.05).Conclusion DESCT can analyze hemodynamic changes in AP quantitatively, which is of great value in evaluating changes in AP of each grade.

Objective: To explore the characteristics of external iliac artery vascular complications atfer renal transplantation and the diagnosis and treatment. Methods: We reviewed the clinical data of 6 patients with of external iliac artery vascular complications atfer renal transplantation from more than 2000 renal transplantation patients in the Transplantation Center of the Third Xiangya Hospital of Central South University from 2001 to 2013, and analyzed the clinical characteristics, diagnosis and treatment. Results: hTe renal allogratf was removed in 5 of the 6 patients due to repeated external iliac arteryhemorrhage: 2 patients were replaced the external iliac artery with reversed autogenous great saphenous vein, 2 patients underwent the bilateral femoral artery bypass surgery, and 1 was repaired the external iliac artery directly. The other 1 was resected the renal allograft and the involved external iliac arteries due to fungal mass in the external iliac artery. Among the 6 patients, except 1 patient died atfer the surgery of the repair of the external iliac artery, the other 5 are all alive. Conclusion: Vascular replacement and artery bypass are effective methods for patients with external iliac artery vascular complications atfer kidney transplantation.

Objective To analyze clinical characteristics of migraine complicated with cerebral edema.Methods We first time reported a case of familiar hemiplegic migraine complicated with diffuse cerebral edema in China,another 19 cases of migraine complicated with cerebral edema were reported in literature.The clinical characteristics of these 20 cases were analyzed.Results In 20 cases,the male:female ratio was 1.2∶ 1,the mean age of onset was (16.0 ± 10.2) years.All 20 cases were complicated with cerebral edema,among whom 8 (40％) were familiar hemiplegic migraine,8 (40％) were sporadic hemiplegic migraine,4 (20％) were migraine with aura.Common neurological symptoms and signs included paralysis (75％),aphasia (65％),disturbance of consciousness (60％),disordered vision (40％),Paresthesia (35％).Eleven cases (55％) had permanent neurological deficit.Conclusions The clinical manifestations of migraine with cerebral edema are complicated,the neurological disturbance is severe and usually has a long duration.The early diagnosis and treatment is beneficial,and preventive treatment in intermittent period is recommended.