Objective To analyze dynamic features of TCM syndrome elements according to the neuropsychological characteristics of cognitive function impairment after ischemic stroke.Methods The hospitalized cerebral infarction patients without dementia were evaluated dynamically with the mini-mental state examination (MMSE) and Montreal Cognitive Assessment (MoCA) (Beijing Version) after IQCODE screens at different phase points.And they were divided into groups according to the severity of cognitive function impairment.The occurring frequency of TCM syndrome elements at all phase points for diagnosis was compared.Results The phase points and TCM syndrome elements closely related with the cognitive function impairment after ischemic stroke were as following:the 3rd day with fire and yin deficiency,the 7th day with yin deficiency,the 1st month with fire,phlegm,and blood stasis,and the 3rd month with fire,phlegm,blood stasis,qi deficiency,and yin deficiency.Conclusion The neuropsychological characteristics of cognitive function impairment of ischemic stroke are closely related with TCM syndrome elements and their evolvement as well as their different combinations.

Hepatocellular carcinoma has a low early diagnostic rate, and there is a lack of highly sensitive and specific tumor markers. In recent years, fluid biopsy technique, represented by circulating free DNA (cfDNA), has become an auxiliary method for the diagnosis of cancer and has attracted more and more attention due to its advantages of noninvasiveness, convenience, and repeatability. With reference to the recent studies in China and foreign countries, this article summarizes and analyzes the advances in cfDNA in the diagnosis and treatment of hepatocellular carcinoma from the aspects of biological characteristics, detection techniques, and clinical application, so as to provide a basis for clinical diagnosis and treatment.

Objective To explore the clinical characteristics of pattern of phlegm heat and bowel excess in acute stage of stroke involving meridian-collateral or involving zang-fu organs from the aspects of severity of neurological impairment, pattern distribution at different time points and duration of bowel excess.Methods The basic information,scores of National Institute of Health Stroke Scale(NIHSS), whether bowel qi being unobstructed or not,duration of bowel excess,and pattern distribution at different time points(onset for 1 d to 3 d,onset for 4 d to 7 d and onset for 8 d to 14 d)were inputted based on structured data by using retrospective analysis of medical records in 204 cases of pattern of phlegm heat and bowel excess of stroke involving meridian-collateral(meridian-collateral group)and 217 cases of pattern of phlegm heat and bowel excess of stroke involving zang-fu organs(zang-fu organ group).The clinical characteristics from the data were compared, analyzed and studied.Results The severity of neurological impairment except of conscious state was significantly higher in zang-fu organ group than that in meridian-collateral group(P<0.01).The average duration of unobstructed bowel qi was significantly longer in zang-fu organ group than that in meridian-collateral group(P<0.01), and the percentage of patients with throughout obstructed bowel qi was significantly higher in zang -fu organ group than that in meridian-collateral group in acute stage of stroke.The pattern of phlegm heat and bowel excess showed a decline trend after stroke onset for 1 d to 14 d in meridian-collateral group, and showed an ascending trend after stroke onset for 1 d to 7 d and a decling trend after stroke onset for 8 d to 14 d in zang-fu organ group.The cases of pattern of qi deficiency with blood stasis or pattern of collaterals blocked by wind -phlegm and static blood were significantly higher in meridian-collateral group at 4-14 d than those at 1-3 d,and cases of pattern of wind stirring due to yin deficiency,pattern of phlegm heat and blood stasis or pattern of wind phlegm and blood stasis were significantly higher in zang-fu organ group at 8 -14 d than those at time point 1.Conclusion The duration of pattern of phlegm heat and bowel excess is posi-tively correlated to the severity of stroke,that is,neurological impairment is more serious and duration is longer in patients with pattern of phlegm heat and bowel excess of stroke involving zang -fu organs.

Objective:To compare the effects of gelatin(Gel)and polydopamine(PDA)modification of polycaprolactone(PCL)on the biological behaviour and osteogenic function of osteoblasts.Methods:PCL electrospun membranes were prepared by electrostatic spinning technique,PCL surface was modified by Gel and PDA respectively as G/PCL and D/PCL with chemical self-assembly tech-nique,and the physicochemical properties of the electrospun membranes were characterized by scanning electron microscopy(SEM),Fourier infrared spectroscopy(FTIR),X-ray photoelectron spectroscope(XPS)and contact angle measurement.The MC3T3-E1 cell adhesion morphology was observed by SEM,immunofluorescence staining followed by confocal microscopy(CLSM),cell proliferation at 1,3 and 5 d was tested by CCK-8 assay,alkaline phosphatase(ALP)staining,alizarin red staining and qRT-PCR were used to detect osteogenic gene expression of the cells.Results:A coating of PDA particles was observed on the surface of D/PCL film.FTIR and XPS showed that the characteristic peaks of Gel and PDA,and there was no obvious droplets on the surface of G/PCL and D/PCL ob-served by contact angle test.Cell density of G/PCL group was higher,the adhesion morphology was good and pseudopods were obvi-ous.CCK-8 assay showed the highest proliferation of the cells on G/PCL(P＜0.05).ALP and alizarin red staining of the cells were stronger in D/PCL group than in the other 2 groups.qRT-PCR results showed that the mRNA expression of ALP,COL-1,RUNX2 and OCN was higher in the D/PCL group than in the other 2 groups.Conclusion:Both Gel and PDA modification can enhance the cell adhesion,proliferation and osteogenic properties of PCL scaffolds,Gel modification may induce a more pronounced proliferative effect and PDA modification more pronounced osteogenic effect.

China is currently in an accelerated stage of population aging, and brain diseases pose a significant threat to the health of the elderly. " Preventing brain aging and maintaining brain health" has become a high-level goal of healthy aging. During the process of aging, the physiological and psychological states of elderly people change, making them prone to nervousness and exhaustion, which can disturb the brain spirit, damage the brain collaterals, and severely endanger brain health. Starting from the holistic view of cultivating both body and spirit in traditional Chinese medicine, based on the physical and mental characteristics of the elderly, this paper applies the concept and method of " relaxation and tranquility" in the protection of elderly brain health, focusing on maintaining relaxation and tranquility in both physical and mental aspects. Specific measures include emphasizing subjective consciousness, relaxing the heart and calming down; utilizing the daoyin method, relaxing the body and calming down, combining relaxation and tranquility, cultivating both body and spirit to prevent diseases and protect the brain, which enables the elderly to have a healthy mind and body, a sense of happiness and fulfillment, and to age gracefully. Simultaneously, advocating for tranquility is also called " respect" for relaxation, following nature to understand constant changes, and improving one′s ability to think positively in old age, in order to expand ideas for the protection of elderly brain health.