The purpose of this study was to develop a robotic system for image-guided percutaneous interventions using ultrasound (US) or computed tomography (CT) for needle biopsy, ablation, cryotherapy, and other needle procedures. The conventional operation of puncture was analyzed firstly, and a compact robot was then designed for manipulating a needle or other slender surgical instrument in the confined space. Its distinctive characteristic is the decoupled motion capability correlated to the positioning and orientation steps of the percutaneous intervention. This approach allowed each step of the intervention to be performed using a separate mechanism of the robot. One major advantage of this kinematic approach was patient safety.

Objective: To study the expression of PDCD5 mRNA and its significance in esophageal cancer in Xinjiang Kazakh and Han nationality. Methods: RT-PCR was used to detect the mRNA of PDCD5 in 40 cases of esophageal cancer (18 cases of Kazakh, 22 cases of Han). Results: The positive rate of PDCD5 mRNA expression in 40 samples of esopha-geal cancer tissue, adjacent tissue, and normal tissue was 80.0% (32/40), 80.0% (32/40) and 87.5% (35/40), respectively, with no significant difference (P>0.05). The Ods of cancer tissues, adjacent tissues, and normal tissues were 0.7644± 0.1444, 0.9341 ±0.1631 and 1.8703±0.4767, respectively. The expression of PDCD5 was significantly increased in cancer tissues compared with that in normal tissues (P<0.05). The expression level of PDCD5 mRNA was not significantly correlat-ed with the degree of differentiation and lymph node metastasis. Conclusion: No significant difference was found in PDCD5 mRNA expression in esophageal cancer between Xinjiang kazakh and Han nationality (P>0.05). The expression of PDCD5 is not correlated with the degree of differentiation, depth of invasion or lymph node metastasis. Detection of PDCD5 mRNA expression in esophageal cancer tissues may provide valuable information for patient prognosis.

Objective To detect the DMD gene mutation sites and the regions of breakpoints in Duchenne/Becker muscular dystrophy (DMD/BMD) patients in northern China. Methods Multiplex amplifiable probe hybridization (MLPA) was used to detect the mutation in 59 cases (51 cases with DMD and 8 with BMD) from northern China and dystrophin gene mutations in their parents. Results From northern China and dystrophin gene mutations 59 families found gene deletions in 33 cases of 59 DMD/BMD patients (55.9%), duplications in 6 cases (10. 2%) and point mutation in one case (1.7%). Intron 44 was most frequently affected (n = 13, 33.3%), followed by intron 50 (n = 11, 28.2%) and intron 45 (n=8, 20.5%). The novel mutations were identified, in two patients including two independent duplications carried by patient D1 149 and a point mutation [5208del(A)] carried by patient D1 65, which were not included in Leiden database. In addition, an exon 22 deletion was found in one patient, which was the first reported case in Chinese patients. Conclusions Deletions are mostly located in the hotspot between exon 45 and 50. Duplications mostly occurred in the 5' end of the gene. Intron 44 is the most frequently affected breakpoint in northern Chinese population.

Objective To explore the clinical features and risk factors of post-concussion syndrome (PCS) in patients after mild traumatic brain injury (mTBI).Methods Two hundred and seventy-six patients with mTBI,admitted to our hospital from December 2016 to June 2018,were chosen in our study;114 patients (41.30％) developed PCS.The epidemiological data,causes and sites of brain injury,clinical symptoms,and duration and time of PCS occurrence were investigated.Multivariate Logistic regression was used to analyze the risk factors of PCS in patients with mTBI.Results The most common syndromes of PCS were headache (89.13％),amnesia (63.77％),dizziness (63.04％) and nausea (57.61％).The incidence rate of PCS was 51.75％ in the first month of injury and decreased with time.Multifactor Logistic regression analysis showed that student (P=0.041,OR=0.36,95％ CI:0.14-0.95),electric bicycle accidents (P=0.043,OR=0.54,95％CI:0.30-0.98),and occipital injury (P=0.022,OR=0.28,95％CI:0.09-0.83) were independent risk factors of PCS of mTBI patients.Conclusion Patients with mTBI,especially those who are students,victims of electric bicycle accidents and victims of occipital injury,should be highly alert to the occurrence of PCS,and need reasonable rest,symptomatic treatments and memory training or other rehabilitation treatments within one month of injury,in order to prevent the development of PCS.