Objective:To evaluate the surface free energy and Streptococcus mutans(S.mutans)adhesion on the surfaces of Co-Cr al-loy specimens fabricated by selective laser sintering(SLS)and casting method(SG and W280)respectively.Methods:The specimens of three groups were polished by SiC sandpaper.Surface morphology test,surface contact angle test and surface adhesion test of S.mu-tans were performed.Results:With the same surface roughness value,the surface contact angle of group SLS,W280 and SG was 66.200 ±3.112,74.212 ±3.206 and 67.238 ±2.047(P >0.05),the surface adhesion of S.mutans colone was 8.88 ±2.90,9.25 ± 2.05 and 8.50 ±1.85(P >0.05),respectively.Conclusion:The cobalt chromium alloy fabricated by SLS and casting method have similar surface performance.

OBJECTIVE:To further enhance the implementation level of new Good Manufacturing Practices (GMP) in phar-maceutical enterprises. METHODS:Based on the new GMP criteria and Guidelines for Risk Assessment of Pharmaceutical Produc-tion Site Inspection,a tracking inspection was conducted for the 31 pharmaceutical enterprises in 11 prefecture-level cities of Guangxi area. Defects of implementing new GMP and their causes were analyzed,and the suggestions were put forward. RESULTS& CONCLUSIONS:In the 31 pharmaceutical enterprises,there were 30 enterprises(96.8%)passed the new GMP tracking inspec-tion. Totally 331 defect items were found,focusing on equipment(76 items,23.0%),factory and facilities(47 items,14.2%),quality control and quality assurance(43 items,13.0%). Causes for defects were mainly institutions and staff training,factory and facilities,equipments management,maintenance and periodic check were far from satisfactory;material and product did not speci-fy a validity period or a retest period;relevant confirmation was incomplete with verification;the quality control,assurance,man-agement,document and production management were not standard and completed;and self-test program was too simple,etc. It is suggested that drug regulatory authorities should establish and improve the legal system,explore new patterns of drug GMP track-ing inspection (such as sudden unannounced inspection,detailed inspection),strengthen inspector team training and unify inspec-tion scale. And the pharmaceutical enterprises should produce drugs in line with the requirements of new GMP,focus on the update of key knowledge,strengthen relevance of training to ensure the drugs quality effectively by multilateral force.

Objective To investigate the variation of vascular cell adhesion molecule-1 (VCAM-1) expression and lipid peroxidation in aorta and plasma of Ins2~ Akita diabetic mice, and to inquire into the relationship between the variation and the cardiovascular diseases in diabetic patients. Methods With the aortic and plasma samples from Ins2Akita diabetic mice, the protein expression levels of VCAM-1 were detected by Western Blot or ELISA, and the levels of lipid peroxides were determined by lipid peroxidation kit. Results Compared with that of normal control, VCAM-1 protein and lipid peroxides levels were significantly elevated in the aorta and plasma of Ins2~ Akita diabetic mice (P

Follicular lymphoma (FL) is a kind of indolent non-Hodgkin lymphoma (iNHL), which origins from follicle germinal center. Multiple researches reported the latest development about treatment of FL in the 58th American Society of Hematology (ASH) Annual Meeting. The outcomes of clinical trials in which GAl01 combined with chemotherapy and bendamustine in combination with 90Y-ibritumomab tiuxetan for the advanced stage FL were exciting. Relapsed/refractory FL patients who receive tandem autologous followed by nonmyeloablative allogeneic transplantation could acquire preferable remissions. With the emerging of novel drugs including inhibitor of bcl-2 or PI3K and antibody drug conjugate, more and more improvements of efficacy and remission were made in the treatment of relapsed/refractory FL therapy and remission.

Ductular reaction (DR) is a phenomenon that refers to impaired hepatocellular proliferation and activated hepatic stem cells at times of demand such as hepatic regeneration.The components of ductular reactions are thought to be derived from activated hepatic progenitor cells,intermediate hepatobiliary cells,and reactive ductular cells.DRs are thought to play two roles in hepatocarcinogenesis:their hepatic progenitor cells may undergo malignant transformation and migration,and they may contribute to the development of the tumor's microenvironment.Exploring DR phenotype,cellular diversity,and relationship to hepatocarcinogenesis will further enhance our understanding of the pathogenesis of human hepatocellular carcinoma with an ultimate goal of developing new therapies.

AIM: To investigate the influence of Sijunzi Decoction(Radix et Rhizoma ginseng,Rhizoma atractylodis macrocephalae,poria,Radix et Rhizoma glycyrrhizae) on the pharmacokinetics of Levofloxacin(LVFX) in rats with deficiency of spleen. METHODS: Twenty-four rats were randomly divided into four groups: NS(normal rats given 0.9% saline solution),NS+SJZT(normal rats given 0.9% saline solution and Sijunzi Decoction),R(rats pretreated with Reserpine) and R+SJZT(rats pretreated with Reserpine and then cured with Sijunzi Decoction).After a single oral administration of LVFX 20 mg/kg,blood samples were collected at different intervals.The concentrations of LVFX plasma were determined by HPLC.Pharmacokinetic parameters were determined from the plasma concentration-time data. RESULTS: Reserpine led to the syndromes similar to the deficiency of spleen,a traditional Chinese medicine syndrome.The pharmacokinetic parameters of LVFX in NS,NS+SJZT,R and R+SJZT groups were as follows: AUC_((0-∞))=(8.55 ?0.99),(7.41?1.39),(4.68?0.95) and(7.89?1.41)mg/(L?h),respectively and C_(max)=(3.31?0.63),(2.38?1.15),(1.29?0.45) and(3.35?1.15) mg/L,respectively.Compared with the parameters of LVFX in NS group,Reserpine markedly decreased AUC_((0-∞)) and C_(max) of LVFX(P

Objective To evaluate the efficacy of automated medication management system in increasing clinical anesthesia management specification.Methods Sixty registered anesthesiologists of both sexes in our hospital,residents or attending physicians,with 1-10 yr of work experience,were divided into 2 groups (n =30 each) using a random number table:traditional medication management mode group (group T) and automated medication management system group (group A).In the clinical anesthesia work,traditional artificial management mode was used in group T,the mode of automated medication management system was used in group A,and 2 licensed pharmacists worked in the center pharmacy of our hospital were introduced for each group.Time of taking medicine was recorded by licensed pharmacists,and the error rates of taking medicine and recording were determined by licensed pharmacists.Results Compared with group T,the error rates of taking medicine and recording were significantly decreased (P＜0.01),and no significant change was found in the time of taking medicine in group A (P＞0.05).Medication errors including taking wrong anesthesia drug,wrong prescription recording,wrong names of the common drugs and drug omission were not detected in group A.Conclusion Application of automated medication management system can reduce the occurrence of errors made in taking medicine and recording,specify medication management and increase the quality of clinical anesthesia management.

Objective To study the clinical characteristics and relative risk factors of hypertensive inpatients aged over 80 years.Methods From February 2003 to May 2012,336 hypertensive inpatients aged over 80 years were enrolled in the study.Medical history and family history including height,weight,blood pressure,fasting glucose,total cholesterol,triglyceride,high-density lipoprotein cholesterol,low-density lipoprotein cholesterol,creatinine,urea nitrogen,uric acid and urine protein levels were recorded and analyzed statistically.Results The elderly hypertensive inpatients over 80 years old had systolic hypertension with widening pulse pressure and the decline in glomerular filtration rate.The incidence of dyslipidemia,atrial fibrillation,microalbuminuria,coronary heart disease,cerebrovascular disease were 56.5 ％ (190/336),13.4 ％(45/336),15.8％ (53/336),41.4％ (139/336) and 24.1％ (81/336),respectively.Whereas there was no significant sex difference (P＞0.05).A significant difference was found in the incidence of left vetricular hypertrophy between males (10.4％,25/240) and females (22.9％,22/96) (x2 =8.91,P＜0.01).The prevalence of diabetes in males (46.3％,111/240) was significantly higher than in females (28.1％,27/96) (x2=9.38,P＜0.01).From the 336 patients in the study,311 (92.6％)were treated by antihypertensive agents.The antihypertensive drugs included diuretics (37 patients,11.9％),calcium channel blocker (182 patients,58.5％),angiotensin receptor antagonists (89 patients,28.6％),angiotensin converting enzyme inhibitors (68 patients,21.9％),β-blockers (92 patients,29.6％),α-blockers (31 patients,10.0％),either alone (109 patients,35.0％) or in combination (202 patients,65.0％).96 (30.9％) patients were medically treated for dyslipidemia (drugs including statins).151 (48.6％) patients were treated with antiplatelet drugs.Conclusions The elderly hypertensive inpatients,over 80 years old,were accompanied by multiple risk factors and target organ damage,and most of them was treated with antihypertensive agents in combination.

OBJECTIVE Based on the epidemiologic data, to provide the effective measures to control nosocomial infection in field hospital. METHODS The views in literatures on medical treatments in war conditions and the practical measures for control and prevention of nosocomial infection were summarized. RESULTS Nine steps must be taken in order to effectively control and prevent nosocomial infection, including making plans and measures, preparing important materials, training medical staff, prevention educating, sterilizing, cutting off pathogenic source, monitoring, reasonable antibiotics applying, and protecting susceptible people. CONCLUSIONS In field hospital, effective prevention and control of nosocomial infection is the premises of improving healing rate and rejoining rate of the wounded.

AIM: The objectives of the present study were to examine the effect of iron on relaxation of isolated rat aortic rings,and to elucidate the underlying mechanism. METHODS: The thoracic aortic rings of male Sprague-Dawley rats were mounted on bath system. Vasodilatation of aortic rings preconstricted with 10 -6 mol/L of phenylephrine (PE) was measured. RESULTS: (1) Exposure of endothelium-intact aortic rings to ferric ammonium citrate (FAC) for 30 min caused a significant reduction in the relaxation response to acetylcholine (ACh). Pretreatment with L-arginine (L-Arg) before incubation with FAC did not reverse the inhibition of relaxation response to ACh completely. (2) In endothelium-intact aortic rings,L-Arg relaxed the PE preconstricted vessels. Exposure to FAC for 30 min caused the decrease in the relaxation response to L-Arg. There was no difference in the relaxation response to nitric oxide donor,sodium nitroprusside, between endothelium-denuded arteries treated with or without FAC. (3) Dimethyl sulfoxide had no effect on the inhibition of relaxation to ACh by FAC in endothelium-intact rings. Pretreatment of arteries with glutathione and catalase prevented the decrease in relaxation responses to ACh induced by FAC. (4) The nitric oxide synthase activity was (56.49?2.49)?10 3U/g protein in normal aorta with endothelium,while after incubation with FAC for 30 min,it reduced to (25.15?5.75)?10 3U/g protein ( P