Presently, with the development of tissue engineering technology, medical workers have seen a more effective and safer therapy to repair and reconstruct tissue defects. Many domestic and abroad animal experiments have confirmed that it is feasible, effective and superior to use tissue-engineered bone and cartilage for repairing tissue defects. There have been achieved considerable results using tissue-engineered bone and cartilage to repair tissue defects and reconstruct tissue functions through the animal experiments and initial clinical studies. Compare with traditional operations, tissue engineering technology has a broader clinical application, especially in orthopedics, department of stomatology, cranio-facial surgery and ear, nose and throat, department. However, through the research, we recognize some deficiencies of tissue engineering, such as single tissue components, lack of biological activity, limited for scaffolds, tissue regeneration and productions.

Objective To investigate the value of distinguishing small clear cell renal cell carcinoma and high-attenuation renal cysts by applying unenhanced CT value and homogeneity of the esions.Methods Retrospective analysis of 38 cases of small renal cell carcinoma confirmed by operation and pathology and 58 cases of high-attenuation renal cyst that were confirmed by enhanced CT,and measured CT value and homogeneity of each mass,and did statistical analysis for corresponding datas.Results The CT value of small clear cell renal cell carcinoma was (35.5±10.1)HU (range 20-60.3 HU).The CT value of high-attenuation renal cysts was (70±1 5.2)HU (range 29-95 HU).Difference of the two groups had statistical significance (P <0.001).For high-attenuation re-nal cysts,uniform density often can be seen(47 cases,81.03%).For small clear cell renal cell carcinoma,uneven density often can be seen (29 cases,76.32%).Difference of the two groups had statistical significance (P <0.001).When optimal cut-off of CT value determined by ROC was 5 1.5 HU,the sensitivity and specificity were 89.7% and 92.1%,respectively.Conclusion Unenhanced CT may be helpful in distinguishing small clear cell renal cell carcinoma and high-attenuation renal cysts,which can provide reasona-ble suggestions on the next identification examinations.

OBJECTIVE:To optimize the extraction and purification technology of resveratrol from Polygonum cuspidatum. METHODS:By alkali extraction and acid precipitation,an orthogonal experiment was conducted to optimize the extraction and purification technology taking the ratio of the CaO to the raw material,the ratio of solid material to the extraction solvent,the kinds of acid and the extraction time as factors and the weighted mean of the content and yield as comprehensive score index.RESULTS:The optimal extraction and purification technology was as follows:the ratio of CaO to the solid material was 9:100;the ratio of solid material to solvent was 1:8;the acid was hydrochloric acid and the extraction time was 45 min.CONCLUSION:The optimized extraction and purification technology of resveratrol from Polygonum cuspidatum is characterized by energy source - saving and simple in operation,and it is suitable for industrial production.

Newer retinal imaging technologies help us in understanding the pathogenesis of many retinal pathologies,such as diabetic retinopathy,age related macular degeneration,glaucoma and uveitis.Early detection of these retinal diseases can prevent the onset of progressive vision loss,and aid in the development of new treatment options.Retinal functional imager (RFI) is an unique and noninvasive functional imaging system.Unlike most of the available newer retinal imaging tools,the RFI not only shows retinal structural changes,but can directly monitor functional changes and measure hemodynamic parameters,such as retinal bloodflow velocity,oximetric state,etc.This article reviews the utility progress of RFI in various retinal diseases.

Novel coronavirus (2019-nCoV) is the pathogen of COVID-19. Some severe cases may suffer from respiratory failure or even death, which poses a great challenge to global public health. 2019-nCoV proteins not only participate in virus proliferation, but also play an important role in antagonizing host innate immune response, especially interferon response. In this paper, 2019-nCoV proteins involved in regulating host interferon response and the complex interaction between 2019-nCoV and interferons were summarized, aiming to provide a theoretical reference for the prevention and control of COVID-19.

Cyprinid herpesvirus 3 (CyHV-3) is the causative agent of an extremely contagious and aggressive disease afflicting common corp Cyprinus carpio L. termed koi herpesvirus disease (KHVD). Since it was first reported in 1997, the virus has spread worldwide rapidly, leading to enormous financial losses in industries based on common carp and koi carp. This review summarizes recent advances in CyHV-3 research on the etiology, epidemiology, pathogenesis, diagnosis, prevention, and control of KHVD.
Animals ; Fish Diseases ; diagnosis ; virology ; Fishes ; classification ; virology ; Herpesviridae ; genetics ; isolation & purification ; physiology ; Herpesviridae Infections ; diagnosis ; veterinary ; virology

Objective To observe the clinical efficacy of thread embedding at the holographic point Kidney of the second metacarpal bone in treating tinnitus.Method Eighty-two patients with tinnitus were randomized into a treatment group and a control group, 41 cases in each group. The treatment group was intervened by thread embedding at the holographic point Kidney of the second metacarpal bone, while the control group was by intragluteal injection with Mecobalamin 0.5 mg. The Tinnitus Handicap Inventory (THI) was compared before intervention and after 1 treatment course.Result The THI scores were significantly changed in both groups after intervention (P<0.01). The change of THI score after intervention in the treatment group was significantly different from that in the control group (P<0.05).Conclusion Thread embedding at the holographic point of the second metacarpal bone in an effective approach in treating tinnitus.

Objective: Observing the effect of injecting 5- Fu into the mass of breast hyper-plasia. Methods: We randomly divide the 80 mastopathy patients into two groups ,40 patients ac-cepted the 5- Fu local injection (experiment group),and 40 patients taken orally rubixiaopian and ta-moxifen(control group). The clinical effects of the breast pain and breast lump were observed. Re-sults: The total effect of 5- Fu local injection into the lump of the mastopathy patients was 92.5 % , while control group was 75.0% , its difference was significant(P0.05)in the treatment of mastopathy lump. Conclusion: 5- Fu local injection had obvious effects to breast pain and breast lump of mastopathy.

Aim To explore the effects of Yupingfeng polysaccharide(YPF-P)on T cells of adjuvant arthritis(AA)in rats.Methods Freund's complete adjuvant was used to induce AA in rat.Secondary paw swelling of AA rats was measured with volumeter.Peripneral blood lymphocyte proliferation response induced by concanavalin A(ConA)was examined with MTT assay.The CD4+ and CD8+ T cell in peripheral blood were detected by flow cytometer.ELISA method was applied to determine the content of factor IFN-? and radio-immunity assay of factor IL-4.RT-PCR procedures were utilized to determine the expression of IFN-? mRNA.Results 80,160 mg?kg-1 YPF-P could singnificantly inhibit the secondary paw swelling of AA rats.The suppressesion of lymphocyte proliferation of peripheral blood T cell in AA rats was reversed.After treatment by YPF-P,the number of CD4+ cell and the ratio of CD4+/CD8+ markedly decreased.The level of IFN-? in YPF-P treating group was obviously decreased in comparison with that of model control group,while the level of IL-4 was increased.The mRNA expression of IFN-? was inhibited as well.Conclusion Yupingfeng polysaccharide can regulate the turbulence of periphery abnormal T cell subgroups of adjuvant arthritis rats and can recover Th1/Th2 balance to move towards Th1,which may be one of its mechanisms of inhibiting the secondary paw swelling.

Objective To evaluate the molluscicidal effect of Rongbao in schistosomiasis endemic areas in Mianyang City. Methods Three Oncomelania hupensis snail habitats with the similar snail status were selected and sprayed with Rongbao,ni-closamide,and fresh water,respectively. Then the snail status in the three fields was surveyed before the spraying and 7,15,30 and 60 days after the spraying,and the molluscicidal effects of different molluscicides were compared. Results The reducing rates of densities of living snails in the field sprayed with Rongbao were 94.4%,95.9%,98.2%and 98.8%,7,15,30 and 60 days after the spraying,respectively. The reducing rates of the densities of living snails in the other field sprayed with niclosamide were 94.0%,94.0%,89.9%and 92.2%in above-mentioned days,respectively. In the 30 days and 60 days after the spraying,the reducing rates of densities of living snails in the field sprayed with Rongbao were significantly higher than those sprayed with ni-closamide(χ230 d=8.18,χ260 d=3.97,Both P<0.05). Conclusion The short-term molluscicidal effect of Rongbao is similar to that of niclosamide,but the long-term effect of Rongbao is better than that of niclosamide.